Query: CNT0023848
Subject: sp|F4I4W2|HCS2_ARATH Biotin--protein ligase 2 OS=Arabidopsis

OS=Arabidopsis thaliana GN=HCS1 PE=1 SV=2

 Score =  435 bits (1119), Expect = e-150,   Method: Compositional matrix adjust.
Identities = 205/334 (61%), Positives = 262/334 (78%), Gaps = 5/334 (1%)

Query: 38 SSVGME-DSAFLIVLRGKSRSEEEYASSLKHKKSLRLPDGXXXXXXXXXXXXN---DATK 93
S+ ME D++ +VL GKS E E A LK+K SL+LPD N D
Sbjct: 35 SASAMESDASCSLVLCGKSSVETEVAKGLKNKNSLKLPDNTKVSLILESEAKNLVKDDDN 94

Query: 94 TFQIDSYMDILSTHQFGRLLIWSPRISSTHDLVSLNFCELPLGTACIADVQVKGRGRAKN 153
+F + +M+ + TH+FGR LIWSPR+SSTHD+VS NF ELP+G+ C+ D+Q KGRGR KN
Sbjct: 95 SFNLSLFMNSIITHRFGRFLIWSPRLSSTHDVVSHNFSELPVGSVCVTDIQFKGRGRTKN 154

Query: 154 VWQSPVGCLLFSFTLQMDNGRIVPHVQYVVSLAVTEAIKVICKEKGLPDLDVKIKWPNDL 213
VW+SP GCL++SFTL+M++GR+VP +QYVVSLAVTEA+K +C +KGLP +DVKIKWPNDL
Sbjct: 155 VWESPKGCLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLPYIDVKIKWPNDL 214

Query: 214 YLNGLKVGGILCTSTYRSKKFNVSAGIGLNVDNHEPTTCLNAVLQKLTSGAQCLRREHIL 273
Y+NGLKVGGILCTSTYRSKKFNVS G+GLNVDN +PTTCLNAVL+ + + L+RE IL
Sbjct: 215 YVNGLKVGGILCTSTYRSKKFNVSVGVGLNVDNGQPTTCLNAVLKGMAPESNLLKREEIL 274

Query: 274 AAFFERFEVLFDVFINQGFQALEEVYYATWLHSGQKVVIEEKNECGLPQKSVVTVQGLTP 333
AFF +FE FD+F++QGF++LEE+YY TWLHS Q+V++E+K E + Q +VVT+QGLT
Sbjct: 275 GAFFHKFEKFFDLFMDQGFKSLEELYYRTWLHSEQRVIVEDKVEDQVVQ-NVVTIQGLTS 333

Query: 334 SGYLLAVDDEDKRYELHPDGNSFDFFQGLIRRKV 367
SGYLLAV D+++ YELHPDGNSFDFF+GL+RRK+
Sbjct: 334 SGYLLAVGDDNQMYELHPDGNSFDFFKGLVRRKI 367

Query: CNT0023848
Subject: sp|Q920N2|BPL1_MOUSE Biotin--protein ligase OS=Mus musculus GN=Hlcs

thaliana GN=HCS2 PE=2 SV=1

 Score =  422 bits (1085), Expect = e-145,   Method: Compositional matrix adjust.
Identities = 197/327 (60%), Positives = 256/327 (78%), Gaps = 4/327 (1%)

Query: 44 DSAFLIVLRGKSRSEEEYASSLKHKKSLRLPDGXXXXXXXXXXXXN---DATKTFQIDSY 100
D++ +VL GKS E + A+ LK+ L+LPD N D +F + +
Sbjct: 4 DASCSLVLYGKSSVETDTATRLKNNNVLKLPDNSKVSIFLQSEIKNLVRDDDSSFNLSLF 63

Query: 101 MDILSTHQFGRLLIWSPRISSTHDLVSLNFCELPLGTACIADVQVKGRGRAKNVWQSPVG 160
M+ +STH+FGR LIWSP +SSTHD+VS NF E+P+G+ C++D+Q+KGRGR KNVW+SP G
Sbjct: 64 MNSISTHRFGRFLIWSPYLSSTHDVVSHNFSEIPVGSVCVSDIQLKGRGRTKNVWESPKG 123

Query: 161 CLLFSFTLQMDNGRIVPHVQYVVSLAVTEAIKVICKEKGLPDLDVKIKWPNDLYLNGLKV 220
CL++SFTL+M++GR+VP +QYVVSLAVTEA+K +C +KGL DVKIKWPNDLYLNGLK+
Sbjct: 124 CLMYSFTLEMEDGRVVPLIQYVVSLAVTEAVKDVCDKKGLSYNDVKIKWPNDLYLNGLKI 183

Query: 221 GGILCTSTYRSKKFNVSAGIGLNVDNHEPTTCLNAVLQKLTSGAQCLRREHILAAFFERF 280
GGILCTSTYRS+KF VS G+GLNVDN +PTTCLNAVL+ + + L+RE IL AFF++F
Sbjct: 184 GGILCTSTYRSRKFLVSVGVGLNVDNEQPTTCLNAVLKDVCPPSNLLKREEILGAFFKKF 243

Query: 281 EVLFDVFINQGFQALEEVYYATWLHSGQKVVIEEKNECGLPQKSVVTVQGLTPSGYLLAV 340
E FD+F+ QGF++LEE+YY TWLHSGQ+V+ EEKNE + Q +VVT+QGLT SGYLLA+
Sbjct: 244 ENFFDLFMEQGFKSLEELYYRTWLHSGQRVIAEEKNEDQVVQ-NVVTIQGLTSSGYLLAI 302

Query: 341 DDEDKRYELHPDGNSFDFFQGLIRRKV 367
D++ YELHPDGNSFDFF+GL+RRK+
Sbjct: 303 GDDNVMYELHPDGNSFDFFKGLVRRKL 329

Query: CNT0023848
Subject: sp|P50747|BPL1_HUMAN Biotin--protein ligase OS=Homo sapiens GN=HLCS

PE=2 SV=1

 Score =  170 bits (430), Expect = 2e-45,   Method: Compositional matrix adjust.
Identities = 100/287 (34%), Positives = 160/287 (55%), Gaps = 20/287 (6%)

Query: 91 ATKTFQIDSYMDILSTHQFGRLLIWSPRISSTHDLVSLNFCELP--LGTACIADVQVKGR 148
+++ F +++Y L T + G++++++ S+T L+ E+P +G IA Q +G+
Sbjct: 443 SSEHFSLETYRQNLQTTRLGKVILFAEVTSTTMSLLDGLMFEMPQEMGLIAIAVRQTQGK 502

Query: 149 GRAKNVWQSPVGC----LLFSFTLQMDNGRIVPHVQYVVSLAVTEAIKVICKEKGLPDLD 204
GR N W SPVGC LL L+ G+ +P VQ+++SLAV EA++ I G D++
Sbjct: 503 GRGPNAWLSPVGCALSTLLVFIPLRSQLGQRIPFVQHLMSLAVVEAVRSI---PGYEDIN 559

Query: 205 VKIKWPNDLYLNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNHEPTTCLNAVLQKLT-- 261
+++KWPND+Y + L K+GG+L ST + F + G G NV N PT C+N ++++
Sbjct: 560 LRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLIEEHNKQ 619

Query: 262 --SGAQCLRREHILAAFFERFEVLFDVFINQGFQALEEVYYATWLHSGQKVVIEEKNECG 319
+G + LR + ++A E L D F +QG + +YY W+H GQ+V +
Sbjct: 620 HGAGLKPLRADCLIARAVTVLEKLIDRFQDQGPDGVLPLYYKYWVHGGQQVRLGSTEG-- 677

Query: 320 LPQKSVVTVQGLTPSGYLLAVDDEDKRYELHPDGNSFDFFQGLIRRK 366
PQ S+V GL SG+L ++ +HPDGNSFD + LI K
Sbjct: 678 -PQASIV---GLDDSGFLQVHQEDGGVVTVHPDGNSFDMLRNLIVPK 720

Query: CNT0023848
Subject: sp|O14353|BPL1_SCHPO Biotin--protein ligase OS=Schizosaccharomyces

PE=1 SV=1

 Score =  163 bits (413), Expect = 3e-43,   Method: Compositional matrix adjust.
Identities = 95/287 (33%), Positives = 158/287 (55%), Gaps = 20/287 (6%)

Query: 91 ATKTFQIDSYMDILSTHQFGRLLIWSPRISSTHDLVSLNFCELP--LGTACIADVQVKGR 148
+++ F ++ Y L T Q G++++++ +T L+ + P +G IA Q +G+
Sbjct: 447 SSEHFNLEIYRQNLQTKQLGKVILFAEVTPTTMRLLDGLMFQTPQEMGLIVIAARQTEGK 506

Query: 149 GRAKNVWQSPVGC----LLFSFTLQMDNGRIVPHVQYVVSLAVTEAIKVICKEKGLPDLD 204
GR NVW SPVGC LL S L+ G+ +P VQ+++S+AV EA++ I + + D++
Sbjct: 507 GRGGNVWLSPVGCALSTLLISIPLRSQLGQRIPFVQHLMSVAVVEAVRSIPEYQ---DIN 563

Query: 205 VKIKWPNDLYLNGL-KVGGILCTSTYRSKKFNVSAGIGLNVDNHEPTTCLNAVLQKLTSG 263
+++KWPND+Y + L K+GG+L ST + F + G G NV N PT C+N ++ +
Sbjct: 564 LRVKWPNDIYYSDLMKIGGVLVNSTLMGETFYILIGCGFNVTNSNPTICINDLITEYNKQ 623

Query: 264 AQC----LRREHILAAFFERFEVLFDVFINQGFQALEEVYYATWLHSGQKVVIEEKNECG 319
+ LR ++++A E L F ++G ++ +YY W+HSGQ+V G
Sbjct: 624 HKAELKPLRADYLIARVVTVLEKLIKEFQDKGPNSVLPLYYRYWVHSGQQV------HLG 677

Query: 320 LPQKSVVTVQGLTPSGYLLAVDDEDKRYELHPDGNSFDFFQGLIRRK 366
+ V++ GL SG+L + + +HPDGNSFD + LI K
Sbjct: 678 SAEGPKVSIVGLDDSGFLQVHQEGGEVVTVHPDGNSFDMLRNLILPK 724

Query: CNT0023848
Subject: sp|P48445|BPL1_YEAST Biotin--protein ligase OS=Saccharomyces

pombe (strain 972 / ATCC 24843) GN=bpl1 PE=3 SV=1

 Score =  139 bits (350), Expect = 4e-35,   Method: Compositional matrix adjust.
Identities = 97/308 (31%), Positives = 145/308 (47%), Gaps = 41/308 (13%)

Query: 91 ATKTFQIDSYMDILSTHQFGRLLIWSPRISSTHDLVSLNFCELP---LGTACIADVQVKG 147
A +F ++ Y +++ +FG +I +P I ST L+ N+ L G + + Q G
Sbjct: 334 AKPSFDLELYAKLINGCRFGLPIIVAPVIRSTQTLLDKNYRFLDSTNTGFTVLGNYQTAG 393

Query: 148 RGRAKNVWQSPVGCLLFSFTLQMD--NGRIVPHV--QYVVSLAVTEAIKVICKEKGLPDL 203
RGR +N+W SP G L FSF + +D N P QY+++LAV I+ G ++
Sbjct: 394 RGRGQNMWVSPYGTLAFSFIINVDAKNFSTTPIALFQYLMALAVVRGIREYA--PGYENI 451

Query: 204 DVKIKWPNDLYL------------NGLKVGGILCTSTYRSKKFNVSAGIGLNVDNHEPTT 251
IKWPND+Y+ +K+ GI+ TS YR ++ G G+NV N PT
Sbjct: 452 PAFIKWPNDVYVRVDKGGINFQGKQYMKLSGIIVTSNYRKNVLHLVVGCGINVSNLGPTV 511

Query: 252 CLNAVLQKLTSGAQCLRR-----EHILAAFFERFEVLFDVFINQGFQALEEVYYATWLHS 306
LN ++ + + R E +LA+ +F+ + + +GF + YY WLHS
Sbjct: 512 SLNTLVDEWNKNSDNPRLEKFSFEKLLASVLNQFDRYHRLLLEEGFSLILPEYYQYWLHS 571

Query: 307 GQKVVIEEKNECGLPQKSVVTVQGLTPS-GYLLAV------DDEDKRYELHPDGNSFDFF 359
Q V L +QG+T G+LLA + K L PDGNSFD
Sbjct: 572 NQTV--------NLASGGKAIIQGITSDFGFLLAQLLNENNEPTTKVVHLQPDGNSFDLM 623

Query: 360 QGLIRRKV 367
+ LI RK
Sbjct: 624 RNLITRKT 631