miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: BLAST


>48397_bra-miR160a:pmrd
          Length = 127

 Score = 99.6 bits (50), Expect = 4e-22
 Identities = 80/90 (88%)
 Strand = Plus / Plus

                                                                       
Query: 12  atgtatatgcctggctccctgtatgccatacactaagttcatcgaccatcggcgacctcg 71
           |||| |||||||||||||||||||||||| | ||||| ||||||||||| |  |||||| 
Sbjct: 19  atgtgtatgcctggctccctgtatgccatcctctaagctcatcgaccattgatgacctcc 78

                                         
Query: 72  gtggatggcgtatgaggagccaagcatatt 101
           ||| |||||||||||||||||| |||||||
Sbjct: 79  gtgaatggcgtatgaggagccatgcatatt 108




>48396_bra-miR160a:pmrd
          Length = 127

 Score = 99.6 bits (50), Expect = 4e-22
 Identities = 80/90 (88%)
 Strand = Plus / Plus

                                                                       
Query: 12  atgtatatgcctggctccctgtatgccatacactaagttcatcgaccatcggcgacctcg 71
           |||| |||||||||||||||||||||||| | ||||| ||||||||||| |  |||||| 
Sbjct: 19  atgtgtatgcctggctccctgtatgccatcctctaagctcatcgaccattgatgacctcc 78

                                         
Query: 72  gtggatggcgtatgaggagccaagcatatt 101
           ||| |||||||||||||||||| |||||||
Sbjct: 79  gtgaatggcgtatgaggagccatgcatatt 108




>47213_ath-miRf10113-akr:pmrd
          Length = 85

 Score = 79.8 bits (40), Expect = 4e-16
 Identities = 73/84 (86%)
 Strand = Plus / Plus

                                                                       
Query: 17  tatgcctggctccctgtatgccatacactaagttcatcgaccatcggcgacctcggtgga 76
           |||||||||||||||||||||||||  || ||  ||||||  ||||  |||||| |||||
Sbjct: 2   tatgcctggctccctgtatgccatatgctgagcccatcgagtatcgatgacctccgtgga 61

                                   
Query: 77  tggcgtatgaggagccaagcatat 100
           ||||||||||||||||| ||||||
Sbjct: 62  tggcgtatgaggagccatgcatat 85




>46934_ath-miR160a:pmrd
          Length = 85

 Score = 79.8 bits (40), Expect = 4e-16
 Identities = 73/84 (86%)
 Strand = Plus / Plus

                                                                       
Query: 17  tatgcctggctccctgtatgccatacactaagttcatcgaccatcggcgacctcggtgga 76
           |||||||||||||||||||||||||  || ||  ||||||  ||||  |||||| |||||
Sbjct: 2   tatgcctggctccctgtatgccatatgctgagcccatcgagtatcgatgacctccgtgga 61

                                   
Query: 77  tggcgtatgaggagccaagcatat 100
           ||||||||||||||||| ||||||
Sbjct: 62  tggcgtatgaggagccatgcatat 85




>48704_mtr-miR160e:pmrd
          Length = 91

 Score = 61.9 bits (31), Expect = 9e-11
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 72  gtggatggcgtatgaggagccaagcatattc 102
           |||||||||||||||||||||||||||||||
Sbjct: 60  gtggatggcgtatgaggagccaagcatattc 90




>48702_mtr-miR160b:pmrd
          Length = 91

 Score = 61.9 bits (31), Expect = 9e-11
 Identities = 31/31 (100%)
 Strand = Plus / Plus

                                          
Query: 72  gtggatggcgtatgaggagccaagcatattc 102
           |||||||||||||||||||||||||||||||
Sbjct: 60  gtggatggcgtatgaggagccaagcatattc 90




>48542_gma-miR160a:pmrd
          Length = 119

 Score = 60.0 bits (30), Expect = 4e-10
 Identities = 30/30 (100%)
 Strand = Plus / Plus

                                         
Query: 72  gtggatggcgtatgaggagccaagcatatt 101
           ||||||||||||||||||||||||||||||
Sbjct: 75  gtggatggcgtatgaggagccaagcatatt 104




>51610_ptc-miR160c:pmrd
          Length = 98

 Score = 56.0 bits (28), Expect = 6e-09
 Identities = 34/36 (94%)
 Strand = Plus / Plus

                                               
Query: 67  cctcggtggatggcgtatgaggagccaagcatattc 102
           |||| |||||||||||||||||||||| ||||||||
Sbjct: 60  cctccgtggatggcgtatgaggagccatgcatattc 95



 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 19 tgcctggctccctgtatgccat 40
          ||||||||||||||||||||||
Sbjct: 11 tgcctggctccctgtatgccat 32




>51609_ptc-miR160b:pmrd
          Length = 98

 Score = 56.0 bits (28), Expect = 6e-09
 Identities = 34/36 (94%)
 Strand = Plus / Plus

                                               
Query: 67  cctcggtggatggcgtatgaggagccaagcatattc 102
           |||| |||||||||||||||||||||| ||||||||
Sbjct: 60  cctccgtggatggcgtatgaggagccatgcatattc 95



 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 19 tgcctggctccctgtatgccat 40
          ||||||||||||||||||||||
Sbjct: 11 tgcctggctccctgtatgccat 32




>54541_sly-miR160a:pmrd
          Length = 129

 Score = 54.0 bits (27), Expect = 2e-08
 Identities = 27/27 (100%)
 Strand = Plus / Plus

                                     
Query: 14 gtatatgcctggctccctgtatgccat 40
          |||||||||||||||||||||||||||
Sbjct: 15 gtatatgcctggctccctgtatgccat 41



 Score = 54.0 bits (27), Expect = 2e-08
 Identities = 27/27 (100%)
 Strand = Plus / Plus

                                      
Query: 76  atggcgtatgaggagccaagcatattc 102
           |||||||||||||||||||||||||||
Sbjct: 81  atggcgtatgaggagccaagcatattc 107




>54783_vvi-miR160f:pmrd
          Length = 102

 Score = 52.0 bits (26), Expect = 9e-08
 Identities = 26/26 (100%)
 Strand = Plus / Plus

                                    
Query: 15 tatatgcctggctccctgtatgccat 40
          ||||||||||||||||||||||||||
Sbjct: 7  tatatgcctggctccctgtatgccat 32



 Score = 50.1 bits (25), Expect = 4e-07
 Identities = 28/29 (96%)
 Strand = Plus / Plus

                                        
Query: 72  gtggatggcgtatgaggagccaagcatat 100
           |||||||||||||||||||||| ||||||
Sbjct: 65  gtggatggcgtatgaggagccatgcatat 93




>54942_zma-miR160e:pmrd
          Length = 94

 Score = 48.1 bits (24), Expect = 1e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 16 atatgcctggctccctgtatgcca 39
          ||||||||||||||||||||||||
Sbjct: 6  atatgcctggctccctgtatgcca 29




>54941_zma-miR160d:pmrd
          Length = 142

 Score = 48.1 bits (24), Expect = 1e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 16 atatgcctggctccctgtatgcca 39
          ||||||||||||||||||||||||
Sbjct: 18 atatgcctggctccctgtatgcca 41




>54675_tae-miR160:pmrd
          Length = 148

 Score = 48.1 bits (24), Expect = 1e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 16 atatgcctggctccctgtatgcca 39
          ||||||||||||||||||||||||
Sbjct: 19 atatgcctggctccctgtatgcca 42




>48829_osa-miR160d:pmrd
          Length = 142

 Score = 48.1 bits (24), Expect = 1e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                  
Query: 16 atatgcctggctccctgtatgcca 39
          ||||||||||||||||||||||||
Sbjct: 5  atatgcctggctccctgtatgcca 28




>48658_hvv-miR160a:pmrd
          Length = 344

 Score = 48.1 bits (24), Expect = 1e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                   
Query: 16  atatgcctggctccctgtatgcca 39
           ||||||||||||||||||||||||
Sbjct: 321 atatgcctggctccctgtatgcca 344




>54943_zma-miR160f:pmrd
          Length = 116

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 16 atatgcctggctccctgtatgcc 38
          |||||||||||||||||||||||
Sbjct: 18 atatgcctggctccctgtatgcc 40




>54781_vvi-miR160d:pmrd
          Length = 102

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 18 atgcctggctccctgtatgccat 40
          |||||||||||||||||||||||
Sbjct: 10 atgcctggctccctgtatgccat 32




>53874_ptc-miRf12052-akr:pmrd
          Length = 110

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 17 tatgcctggctccctgtatgcca 39
          |||||||||||||||||||||||
Sbjct: 10 tatgcctggctccctgtatgcca 32




>51611_ptc-miR160d:pmrd
          Length = 103

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 17 tatgcctggctccctgtatgcca 39
          |||||||||||||||||||||||
Sbjct: 9  tatgcctggctccctgtatgcca 31




>51608_ptc-miR160a:pmrd
          Length = 102

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 17 tatgcctggctccctgtatgcca 39
          |||||||||||||||||||||||
Sbjct: 9  tatgcctggctccctgtatgcca 31




>48830_osa-miR160e:pmrd
          Length = 114

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 16 atatgcctggctccctgtatgcc 38
          |||||||||||||||||||||||
Sbjct: 8  atatgcctggctccctgtatgcc 30




>46936_ath-miR160c:pmrd
          Length = 89

 Score = 46.1 bits (23), Expect = 6e-06
 Identities = 23/23 (100%)
 Strand = Plus / Plus

                                 
Query: 17 tatgcctggctccctgtatgcca 39
          |||||||||||||||||||||||
Sbjct: 3  tatgcctggctccctgtatgcca 25




>54946_zma-miR160i:pmrd
          Length = 81

 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 18 atgcctggctccctgtatgcca 39
          ||||||||||||||||||||||
Sbjct: 1  atgcctggctccctgtatgcca 22




>54574_smo-miR160a:pmrd
          Length = 106

 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 19 tgcctggctccctgtatgccat 40
          ||||||||||||||||||||||
Sbjct: 11 tgcctggctccctgtatgccat 32




>48703_mtr-miR160d:pmrd
          Length = 91

 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 19 tgcctggctccctgtatgccat 40
          ||||||||||||||||||||||
Sbjct: 6  tgcctggctccctgtatgccat 27




>46865_aqc-miR160b:pmrd
          Length = 82

 Score = 44.1 bits (22), Expect = 2e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                
Query: 19 tgcctggctccctgtatgccat 40
          ||||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgccat 22




>54947_zma-miR160m:pmrd
          Length = 81

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54945_zma-miR160h:pmrd
          Length = 86

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54944_zma-miR160g:pmrd
          Length = 86

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54940_zma-miR160c:pmrd
          Length = 100

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 7  tgcctggctccctgtatgcca 27




>54939_zma-miR160b:pmrd
          Length = 121

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 21 tgcctggctccctgtatgcca 41




>54938_zma-miR160a:pmrd
          Length = 104

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 10 tgcctggctccctgtatgcca 30




>54780_vvi-miR160c:pmrd
          Length = 106

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 11 tgcctggctccctgtatgcca 31




>54759_ttu-miR160a:pmrd
          Length = 87

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54575_smo-miR160b:pmrd
          Length = 108

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 13 tgcctggctccctgtatgcca 33




>54408_sbi-miR160e:pmrd
          Length = 95

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54407_sbi-miR160d:pmrd
          Length = 100

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54406_sbi-miR160c:pmrd
          Length = 83

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54405_sbi-miR160b:pmrd
          Length = 82

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>54404_sbi-miR160a:pmrd
          Length = 84

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>51364_ppt-miR160f:pmrd
          Length = 138

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 30 tgcctggctccctgtatgcca 50




>51363_ppt-miR160e:pmrd
          Length = 138

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 30 tgcctggctccctgtatgcca 50




>51359_ppt-miR160a:pmrd
          Length = 99

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 11 tgcctggctccctgtatgcca 31




>51334_pga-miR160:pmrd
          Length = 80

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>48833_osa-miR160n:pmrd
          Length = 131

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>48832_osa-miR160m:pmrd
          Length = 82

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcca 21




>48828_osa-miR160c:pmrd
          Length = 99

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 7  tgcctggctccctgtatgcca 27




>48827_osa-miR160b:pmrd
          Length = 139

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 30 tgcctggctccctgtatgcca 50




>48826_osa-miR160a:pmrd
          Length = 88

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 4  tgcctggctccctgtatgcca 24




>48791_oba-miR160:pmrd
          Length = 90

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 4  tgcctggctccctgtatgcca 24




>48635_gra-miR160b:pmrd
          Length = 293

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 18  atgcctggctccctgtatgcc 38
           |||||||||||||||||||||
Sbjct: 272 atgcctggctccctgtatgcc 292




>48634_gra-miR160a:pmrd
          Length = 106

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                                
Query: 18  atgcctggctccctgtatgcc 38
           |||||||||||||||||||||
Sbjct: 85  atgcctggctccctgtatgcc 105




>48306_bdi-miR160:pmrd
          Length = 120

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 18 atgcctggctccctgtatgcc 38
          |||||||||||||||||||||
Sbjct: 17 atgcctggctccctgtatgcc 37




>47338_ath-miRf10238-akr:pmrd
          Length = 89

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 5  tgcctggctccctgtatgcca 25




>46935_ath-miR160b:pmrd
          Length = 89

 Score = 42.1 bits (21), Expect = 9e-05
 Identities = 21/21 (100%)
 Strand = Plus / Plus

                               
Query: 19 tgcctggctccctgtatgcca 39
          |||||||||||||||||||||
Sbjct: 5  tgcctggctccctgtatgcca 25




>54760_ttu-miR160b:pmrd
          Length = 218

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 19 tgcctggctccctgtatgcc 38
          ||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcc 20




>54528_sce-miR160:pmrd
          Length = 180

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 19 tgcctggctccctgtatgcc 38
          ||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcc 20




>51367_ppt-miR160i:pmrd
          Length = 139

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 20 gcctggctccctgtatgcca 39
          ||||||||||||||||||||
Sbjct: 31 gcctggctccctgtatgcca 50




>51360_ppt-miR160b:pmrd
          Length = 103

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 20 gcctggctccctgtatgcca 39
          ||||||||||||||||||||
Sbjct: 12 gcctggctccctgtatgcca 31




>48659_hvv-miR160b:pmrd
          Length = 47

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 19 tgcctggctccctgtatgcc 38
          ||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcc 20




>48544_gma-miR160o:pmrd
          Length = 503

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 19 tgcctggctccctgtatgcc 38
          ||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcc 20




>48543_gma-miR160n:pmrd
          Length = 107

 Score = 40.1 bits (20), Expect = 3e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                              
Query: 19 tgcctggctccctgtatgcc 38
          ||||||||||||||||||||
Sbjct: 1  tgcctggctccctgtatgcc 20