miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: BLAST


>54628_sof-miR159a:pmrd
          Length = 265

 Score =  303 bits (153), Expect = 4e-83
 Identities = 237/262 (90%), Gaps = 6/262 (2%)
 Strand = Plus / Plus

                                                                       
Query: 1   tgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggtg 60
           ||||||||||||| ||||||||||||||||||||||||||||||||| || | | |||| 
Sbjct: 3   tgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggtt 62

                                                                       
Query: 61  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtact 120
           ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| |
Sbjct: 63  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtatt 122

                                                                       
Query: 121 ctagag-ggccggagaagagat----tcatgtggtcgtcagtctttgagataggcttgtg 175
            | ||| ||  ||||| ||| |    ||   |||| ||| ||||||||||||||||||||
Sbjct: 123 ttcgagagggaggagaggagctagactctcatggtggtc-gtctttgagataggcttgtg 181

                                                                       
Query: 176 atttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaagggag 235
            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 182 gtttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaagggag 241

                                 
Query: 236 ctctgcatcctgatccatccat 257
           ||||||||||||||||||||||
Sbjct: 242 ctctgcatcctgatccatccat 263




>54629_sof-miR159b:pmrd
          Length = 266

 Score =  289 bits (146), Expect = 5e-79
 Identities = 237/263 (90%), Gaps = 7/263 (2%)
 Strand = Plus / Plus

                                                                       
Query: 1   tgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggtg 60
           ||||||||||||| ||||||||||||||||||||||||||||||||| || | | |||||
Sbjct: 3   tgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggtg 62

                                                                       
Query: 61  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtact 120
           ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| |
Sbjct: 63  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtatt 122

                                                                       
Query: 121 ct---agagggccg-gagaag--agattcatgtggtcgtcagtctttgagataggcttgt 174
            |   ||||||  | ||| ||  ||| ||    ||| ||| |||||||||||||||||||
Sbjct: 123 ttcggagagggaggagaggagctagactctcacggtggtc-gtctttgagataggcttgt 181

                                                                       
Query: 175 gatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggga 234
           | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 182 ggtttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggga 241

                                  
Query: 235 gctctgcatcctgatccatccat 257
           |||||||||||||| ||||||||
Sbjct: 242 gctctgcatcctgaaccatccat 264




>54631_sof-miR159d:pmrd
          Length = 265

 Score =  274 bits (138), Expect = 3e-74
 Identities = 235/264 (89%), Gaps = 10/264 (3%)
 Strand = Plus / Plus

                                                                       
Query: 1   tgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggtg 60
           ||||||||||||| ||||||||||||||||||||||||||||||||| || | | |||| 
Sbjct: 3   tgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggtt 62

                                                                       
Query: 61  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtact 120
           ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| |
Sbjct: 63  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtatt 122

                                                                       
Query: 121 ctagag-ggccggagaagagat------tcatgtggtcgtcagtctttgagataggcttg 173
            | ||| ||  ||||| ||| |      ||||| ||  ||| |||| ||||||| |||||
Sbjct: 123 ttcgagagggaggagaggagctagactctcatgggg--gtc-gtctatgagatacgcttg 179

                                                                       
Query: 174 tgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggg 233
           || ||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
Sbjct: 180 tggtttgcatgaccgatgagctgcaccgtccccttgctggccgctctttggattgaaggg 239

                                   
Query: 234 agctctgcatcctgatccatccat 257
           ||||||||||||||||||||||||
Sbjct: 240 agctctgcatcctgatccatccat 263




>54632_sof-miR159e:pmrd
          Length = 264

 Score =  258 bits (130), Expect = 2e-69
 Identities = 234/263 (88%), Gaps = 8/263 (3%)
 Strand = Plus / Plus

                                                                       
Query: 1   tgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggtg 60
           ||||||||||||| ||||||||||||||||||||||||||||||||| || | | |||||
Sbjct: 2   tgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggtg 61

                                                                       
Query: 61  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtact 120
           ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| |
Sbjct: 62  gttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtatt 121

                                                                       
Query: 121 ctagag-ggccggagaag----agattcatgtggtcgtcagtctttgagataggcttgtg 175
            | ||| ||  ||||| |    ||| ||    ||| ||| ||||||||||||||||||||
Sbjct: 122 ttcgagagggaggagaggagctagactctcacggtggtc-gtctttgagataggcttgtg 180

                                                                       
Query: 176 atttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaagggag 235
            ||||||||||||| |||||||||||||||||||||||||||||||||||||||| ||||
Sbjct: 181 gtttgcatgaccgaagagctgcaccgtccccttgctggccgctctttggattgaaaggag 240

                                  
Query: 236 ctc-tgcatcctgatccatccat 257
           ||| ||||| |||||||||||||
Sbjct: 241 ctcttgcat-ctgatccatccat 262




>54930_zma-miR159a:pmrd
          Length = 246

 Score =  176 bits (89), Expect = 5e-45
 Identities = 95/97 (97%)
 Strand = Plus / Plus

                                                                       
Query: 156 gtctttgagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggcc 215
           |||||||||||||||||||| ||||||||||||||||||||||||| |||||||||||||
Sbjct: 143 gtctttgagataggcttgtggtttgcatgaccgaggagctgcaccgcccccttgctggcc 202

                                                
Query: 216 gctctttggattgaagggagctctgcatcctgatcca 252
           |||||||||||||||||||||||||||||||||||||
Sbjct: 203 gctctttggattgaagggagctctgcatcctgatcca 239



 Score =  119 bits (60), Expect = 1e-27
 Identities = 85/92 (92%), Gaps = 1/92 (1%)
 Strand = Plus / Plus

                                                                       
Query: 15  gaagcggagctcctatcattccaatgaagggcccttttcatggg-tggttccgctgctcg 73
           ||||||||||||||||||||||||||||||| | ||   | ||| |||||||||||||||
Sbjct: 17  gaagcggagctcctatcattccaatgaagggtcgttccgaagggctggttccgctgctcg 76

                                           
Query: 74  ttcatggttcccactatcctatctcatcatgt 105
           ||||||||||||||||||||||||||||||||
Sbjct: 77  ttcatggttcccactatcctatctcatcatgt 108




>51338_pgl-miR159:pmrd
          Length = 226

 Score =  145 bits (73), Expect = 2e-35
 Identities = 79/81 (97%)
 Strand = Plus / Plus

                                                                       
Query: 160 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctc 219
           ||||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 146 ttgagataggcttgtggcttgcatgaccgaggagctgcaccgtccccttgctggccgctc 205

                                
Query: 220 tttggattgaagggagctctg 240
           |||||||||||||||||||||
Sbjct: 206 tttggattgaagggagctctg 226



 Score = 85.7 bits (43), Expect = 2e-17
 Identities = 74/83 (89%), Gaps = 1/83 (1%)
 Strand = Plus / Plus

                                                                       
Query: 19  cggagctcctatcattccaatgaagggcccttttcatgggt-ggttccgctgctcgttca 77
           |||||||||||||||||||||||| ||   ||||  ||||| ||| | ||||||||||||
Sbjct: 1   cggagctcctatcattccaatgaaaggttgtttttgtgggttggtacagctgctcgttca 60

                                  
Query: 78  tggttcccactatcctatctcat 100
           |||||||||||||||||||||||
Sbjct: 61  tggttcccactatcctatctcat 83




>54392_sar-miR159:pmrd
          Length = 175

 Score =  109 bits (55), Expect = 1e-24
 Identities = 73/79 (92%)
 Strand = Plus / Plus

                                                                       
Query: 162 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 221
           ||||||||| ||||  ||||||||||||||||| ||| |||||||| |||||||||||||
Sbjct: 97  gagataggcctgtggcttgcatgaccgaggagccgcatcgtcccctcgctggccgctctt 156

                              
Query: 222 tggattgaagggagctctg 240
           |||||||||||||||||||
Sbjct: 157 tggattgaagggagctctg 175



 Score = 61.9 bits (31), Expect = 2e-10
 Identities = 37/39 (94%)
 Strand = Plus / Plus

                                                 
Query: 56 gggtggttccgctgctcgttcatggttcccactatccta 94
          ||||||| | |||||||||||||||||||||||||||||
Sbjct: 40 gggtggtacagctgctcgttcatggttcccactatccta 78




>54931_zma-miR159b:pmrd
          Length = 221

 Score = 99.6 bits (50), Expect = 1e-21
 Identities = 79/86 (91%), Gaps = 2/86 (2%)
 Strand = Plus / Plus

                                                                       
Query: 160 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtcccctt-gctggccgct 218
           ||||||| |||||||| |||||||||||||||||||||| ||||||||| | || |||||
Sbjct: 122 ttgagatgggcttgtggtttgcatgaccgaggagctgca-cgtccccttcgttgaccgct 180

                                     
Query: 219 ctttggattgaagggagctctgcatc 244
            |||||||||||||||||||||||||
Sbjct: 181 gtttggattgaagggagctctgcatc 206



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 45/49 (91%)
 Strand = Plus / Plus

                                                            
Query: 54  atgggtggttccgctgctcgttcatggttcccactatcctatctcatca 102
           ||||||||| | ||||||||||||||||||||||| ||| |||||||||
Sbjct: 56  atgggtggtacagctgctcgttcatggttcccactgtcccatctcatca 104




>48820_osa-miR159b:pmrd
          Length = 188

 Score = 89.7 bits (45), Expect = 1e-18
 Identities = 78/89 (87%)
 Strand = Plus / Plus

                                                                       
Query: 162 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 221
           |||||||||||||| ||||||||||| |||||| | | |  | |||||||| || |||||
Sbjct: 100 gagataggcttgtggtttgcatgaccaaggagccgaatcaactccttgctgaccactctt 159

                                        
Query: 222 tggattgaagggagctctgcatcctgatc 250
           ||||||||||||||||||||||| |||||
Sbjct: 160 tggattgaagggagctctgcatcttgatc 188



 Score = 54.0 bits (27), Expect = 5e-08
 Identities = 33/35 (94%)
 Strand = Plus / Plus

                                              
Query: 66  gctgctcgttcatggttcccactatcctatctcat 100
           |||||| |||||||||||||||||| |||||||||
Sbjct: 58  gctgcttgttcatggttcccactattctatctcat 92




>54934_zma-miR159e:pmrd
          Length = 203

 Score = 85.7 bits (43), Expect = 2e-17
 Identities = 74/83 (89%), Gaps = 1/83 (1%)
 Strand = Plus / Plus

                                                                       
Query: 160 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtcccctt-gctggccgct 218
           ||||||| |||||||| |||||||||||||||||||||  ||||||||| | || |||||
Sbjct: 121 ttgagatgggcttgtggtttgcatgaccgaggagctgcctcgtccccttcgttgaccgct 180

                                  
Query: 219 ctttggattgaagggagctctgc 241
            |||||| |||||||||||||||
Sbjct: 181 gtttggagtgaagggagctctgc 203



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 45/49 (91%)
 Strand = Plus / Plus

                                                            
Query: 54  atgggtggttccgctgctcgttcatggttcccactatcctatctcatca 102
           ||||||||| | ||||||||||||||||||||||| ||| |||||||||
Sbjct: 38  atgggtggtacagctgctcgttcatggttcccactgtcccatctcatca 86




>48657_hvv-miR159b:pmrd
          Length = 174

 Score = 77.8 bits (39), Expect = 4e-15
 Identities = 69/79 (87%)
 Strand = Plus / Plus

                                                                       
Query: 162 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 221
           |||||||||||||| ||||||||| |||||||| ||  ||  |||| |||| ||||| ||
Sbjct: 96  gagataggcttgtggtttgcatgatcgaggagccgcttcgatccctcgctgaccgctgtt 155

                              
Query: 222 tggattgaagggagctctg 240
           |||||||||||||||||||
Sbjct: 156 tggattgaagggagctctg 174



 Score = 58.0 bits (29), Expect = 3e-09
 Identities = 32/33 (96%)
 Strand = Plus / Plus

                                           
Query: 66 gctgctcgttcatggttcccactatcctatctc 98
          |||||| ||||||||||||||||||||||||||
Sbjct: 49 gctgcttgttcatggttcccactatcctatctc 81



 Score = 52.0 bits (26), Expect = 2e-07
 Identities = 26/26 (100%)
 Strand = Plus / Plus

                                    
Query: 20 ggagctcctatcattccaatgaaggg 45
          ||||||||||||||||||||||||||
Sbjct: 2  ggagctcctatcattccaatgaaggg 27




>54673_tae-miR159a:pmrd
          Length = 177

 Score = 75.8 bits (38), Expect = 1e-14
 Identities = 71/82 (86%)
 Strand = Plus / Plus

                                                                       
Query: 162 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 221
           |||| |||| |||| |||||||||||||||||| ||  ||  |||| |||| ||||| ||
Sbjct: 96  gagagaggcctgtggtttgcatgaccgaggagccgcttcgatccctcgctgaccgctgtt 155

                                 
Query: 222 tggattgaagggagctctgcat 243
           ||||||||||||||||||||||
Sbjct: 156 tggattgaagggagctctgcat 177



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 33/33 (100%)
 Strand = Plus / Plus

                                           
Query: 66 gctgctcgttcatggttcccactatcctatctc 98
          |||||||||||||||||||||||||||||||||
Sbjct: 50 gctgctcgttcatggttcccactatcctatctc 82




>54653_spr-miR159:pmrd
          Length = 392

 Score = 75.8 bits (38), Expect = 1e-14
 Identities = 38/38 (100%)
 Strand = Plus / Plus

                                                 
Query: 220 tttggattgaagggagctctgcatcctgatccatccat 257
           ||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctctgcatcctgatccatccat 38




>54937_zma-miR159m:pmrd
          Length = 457

 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 33/33 (100%)
 Strand = Plus / Plus

                                            
Query: 220 tttggattgaagggagctctgcatcctgatcca 252
           |||||||||||||||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctctgcatcctgatcca 33




>54674_tae-miR159b:pmrd
          Length = 255

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 217 ctctttggattgaagggagctctgcatc 244
           ||||||||||||||||||||||||||||
Sbjct: 227 ctctttggattgaagggagctctgcatc 254




>48819_osa-miR159a:pmrd
          Length = 272

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 217 ctctttggattgaagggagctctgcatc 244
           ||||||||||||||||||||||||||||
Sbjct: 239 ctctttggattgaagggagctctgcatc 266




>48796_oru-miR159:pmrd
          Length = 274

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 217 ctctttggattgaagggagctctgcatc 244
           ||||||||||||||||||||||||||||
Sbjct: 239 ctctttggattgaagggagctctgcatc 266




>48656_hvv-miR159a:pmrd
          Length = 303

 Score = 54.0 bits (27), Expect = 5e-08
 Identities = 30/31 (96%)
 Strand = Plus / Plus

                                          
Query: 220 tttggattgaagggagctctgcatcctgatc 250
           ||||||||||||||||||||||||| |||||
Sbjct: 1   tttggattgaagggagctctgcatcttgatc 31




>48825_osa-miR159m:pmrd
          Length = 249

 Score = 48.1 bits (24), Expect = 3e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                   
Query: 217 ctctttggattgaagggagctctg 240
           ||||||||||||||||||||||||
Sbjct: 226 ctctttggattgaagggagctctg 249




>46932_ath-miR159b:pmrd
          Length = 196

 Score = 46.1 bits (23), Expect = 1e-05
 Identities = 26/27 (96%)
 Strand = Plus / Plus

                                      
Query: 218 tctttggattgaagggagctctgcatc 244
           |||||||||||||||||||||| ||||
Sbjct: 166 tctttggattgaagggagctcttcatc 192




>54378_pvu-miR159:pmrd
          Length = 174

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 151 tctttggattgaagggagctct 172




>54377_pvu-miR159:pmrd
          Length = 174

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 151 tctttggattgaagggagctct 172




>48699_mtr-miR159a:pmrd
          Length = 168

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 146 tctttggattgaagggagctct 167




>48539_gma-miR159a:pmrd
          Length = 214

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 173 tctttggattgaagggagctct 194




>48395_bra-miR159a:pmrd
          Length = 185

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 161 tctttggattgaagggagctct 182




>48339_bna-miR159:pmrd
          Length = 183

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 159 tctttggattgaagggagctct 180




>46931_ath-miR159a:pmrd
          Length = 184

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 218 tctttggattgaagggagctct 239
           ||||||||||||||||||||||
Sbjct: 161 tctttggattgaagggagctct 182




>48701_mtr-miR159c:pmrd
          Length = 44

 Score = 42.1 bits (21), Expect = 2e-04
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                    
Query: 220 tttggattgaagggagctctgcatc 244
           |||||||||||||||||||| ||||
Sbjct: 1   tttggattgaagggagctctacatc 25




>54777_vvi-miR159c:pmrd
          Length = 190

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 160 tttggattgaagggagctct 179




>54540_sly-miR159:pmrd
          Length = 177

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 157 tttggattgaagggagctct 176




>52093_ptc-miRf10271-akr:pmrd
          Length = 174

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 149 tttggattgaagggagctct 168




>51604_ptc-miR159c:pmrd
          Length = 183

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 154 tttggattgaagggagctct 173




>51603_ptc-miR159b:pmrd
          Length = 187

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 158 tttggattgaagggagctct 177




>51602_ptc-miR159a:pmrd
          Length = 183

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 154 tttggattgaagggagctct 173




>48728_mtr-miR319b:pmrd
          Length = 42

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                   
Query: 221 ttggattgaagggagctctgcatc 244
           ||||||||||||||||||| ||||
Sbjct: 1   ttggattgaagggagctctacatc 24




>48686_ltu-miR159:pmrd
          Length = 345

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 220 tttggattgaagggagctct 239
           ||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctct 20