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mature miRNA: CAGCCCTTCTGCTATCCACAT
species: Picea sitchensis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|138286556|gb|ES248077.1|ES248077 WS0367.C21_K23 SS-B-N-21 Picea sitchensis cDNA clone WS0367_K23 3', mRNA sequence

CAGCCCTTCTGCTATCCACAT
||||||||||||||||||||
GTCGGGAAGACGATAGGTGTT

EST sequence (targeted fragment marked with yellow):
TTTTTTTTTTTTTTTTGCAGTAAAAATGGACGGGTTTTCTATTAAAGGAAAACAGCAATAAATTGACCACTGGGCCAAATACATTTTGCCTGGCTCTATCTGAGAATCCTAAAACGATATGTCGCAGGACCTCACTCCGGCCTAAAAACCCATGGGAGGGTTTCGGTTAAAGAAAAAAGACAATAAATTGAAATGCTTGTGACCCAAAATAAAAGCGAGATCATACCTGGAGGGTTTCTCGAATTGGGTGCAGAGAACAGAGGTCAGAAAACTCCCATCGGTTAAGCAAGAAGGATTCTAAAACATTTTTCAAAATTTCAAATCAGATCCAAAGGATTTGAGCATTTGAAATACTTTGAGAACATAAATAATATACCGAGTCTTTTACGGGACAGGAAACTTTTGAGCCTTGGTCCATAATTTTTTATTGCCGTCGAAGAAATTGATGTGAGCAAACTAGTACTGTTGTGGATAGCAGAAGGGCTGGTAAAGAAAAAACAGGCAACCCATTTAATGAGTATTGCCATTAAAATGCACTGCTCGAACACAGTCGCTTGATCTCGAACAGCCAACATCTTGCAATAAAATGCCTCTCATGGAATCTCTATTAGATGGGCTTCGTACTGTTTACTAACTAACCCTTCTATATCCACATCTGTACACTCTGAACGCATCAATTTCTTCATACTGCAAGAAAACAGCTCCCCATCCCTGCAAAATTTGCCATGGTGCCATCGATTGGTGATCAAAACGATAAGGCTCGGTTCATGAGGATAAGGGCAGAATGAGGGATATGATATCCTGAATAAACACCCGTTACCAACAATGCATCCATAAATCTACCATTTATGCATTCACACGGGATGGACA



gi|149964034|gb|ES864062.1|ES864062 WS027101.CR_N15 SS-IL-A-FL-14 Picea sitchensis cDNA clone WS027101_N15 3', mRNA sequence

CAGCCCTTCTGCTATCCACAT
|||||||||||||||||||||
GTCGGGAAGACGATAGGTGTA

EST sequence (targeted fragment marked with yellow):
AAGAGCTCTTTTTTTTTTTTTTTTCTAAAACATATTTCAAATTTCAAATCAGACTAAGCATTTTAACCATCTGAAATATTTTGGCAATACAAATAGTATAGCCGAGTCATTTATGGGACAGGAAACTTTGATGCCCTCTTCCATATTTTTTTGCTGAGACTACAGATTGCCTTCAAAGAAATTGATGTGGACAAATTAGTACGAATGTGGATAGCAGAAGGGCTGGTAAAGAGCAAAAGGTAACCCAATGAGCATGGCCATCTAAATACAGCTGCTGGTACACAGTTGCTTGATCTCAAACAACCAACAATCTACAATAAGATGGCCCTTATGCAATCTGCATCAAATGGGTTTTGTATTTTCTGTTACCTAGCCCTTCCATATCCACATCTGTACAATATGAACACATCAATTTCTTCATATTGCAAGAAACAGCGCCTGAACCCTGCAAAACTGGTGACTGGCGCAAGTTGAATGGCCGCAGAAACCATGTTAACCGGG



gi|70299299|gb|DR522364.1|DR522364 WS02711.C21_I14 SS-IL-A-FL-14 Picea sitchensis cDNA clone WS02711_I14 5, mRNA sequence

CAGCCCTTCTGCTATCCACAT
||| ||||||| ||||||||
GTCTGGAAGACTCTAGGTGTA

EST sequence (targeted fragment marked with yellow):
TTTGCCATTGGCGATAACTGTAATAGCCTCAACAATGAAGAGCAAGAGAGCTATAAATGACTGGAAGTTTGCCTTAAGGCAAATGCAAAAAGTGGATTTAAGTTTTCCAATTACTCATCCTCGCATTGATCGAGATTTGTATCAGCGATTGAGATGGAGTTACGATGCTTTGCCCACCACTGCTGATTTGAAAAACTGCTTTCTTTACTGTGCCCTGTTCCCCGAAGATGCACTAATTCGCGAGGAAGATTTGGTCCAAATGTGGATCTCAGAAGGTCTGATTAAGACCAGTGATGGGGACTACGACTATTTATTGGACACTGGGCGCAGCTATGTAAAGTTGCTTCTAGATCGTTGCTTCTAGATCGTTGGACACTGGGCGCAGCTATCTAAAGTTGGACACTGGGCGCAGCTATCTAAAGTTGGACTACGACTATTTATTGGACACTGGGCGCAGCTATCTAAAGTTGCTTCTAGATCGTTGCTTGCTGAATGGGCCTTTTAGCTTTAATGCTGATCGGGTTATTGATAACCATAGCTTTATTTCTGTTCACGATGTAATCCGCGATATGGGAATATATATTGGAGAGAAAGAGGAAAATTATGCATGTAGGGCAGGTCAAAGGCTTCAACATTTCCCCGATATGGAGGCCCTTGCTGATTGCAAACGATTATCAGTTCGGAACAACAATATTACGTCTTTGCCGACAAAGGAATTTAGATGTCCAAAATTAGTTTCTTTATTATTAGGAGGAAATACAGAATTGAAAACAATTCCAGAAGGCTTTTTCCTCAACTTCCCCTCTTTGAAGGTATTGGACCTAGGCTGAACAGCGTTAACATCATTGCCAACATCATTATGGCAATTGATACACCTCGA