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mature miRNA: TACCAAAGAAGATTTGCCCCA
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|119009714|gb|DV447156.1|DV447156 CV01021A1H08.f1 CV01-normalized library Manihot esculenta cDNA clone CV01021A1H08.f1, mRNA s

TACCAAAGAAGATTTGCCCCA
. ||||||||||.||.|| ||
GCGGTTTCTTCTGAATGGAGT

EST sequence (targeted fragment marked with yellow):
CAAATCTTATCGGGGCGACAGAGATTTCAGTTGNNNNNTTGGCAGAGTCATTAATGTTGGGAGTTGCAATAATTGCAAATGACATTTCACATTTTGGATTTTTAGCAATGCTGTCACTTTTATCAGTAGTTGGGGCAGCATGGATGTTTTGTCGATGGTTGTCGAATCCAACAGATGAACAAAGAAGTCTTTTCTCTATCAGCCTAGGTTATAATTTTTAGCTTTCCAATTCGAAGGAACACAGATCAAGGCTTTTAACTGTTACAGGAGCCAAGCTAAAGCCATTTCAGAATACATGTTCCAGAATCAGCATGTGTATAGCAGCCACAGAAGGCCTTGAGGTAAGTCTTCTTTGGCGACTTAGTGGGTCAGAGTTTCTGTAATTGCACGGCTCAGTTGAAATAGCATTGTCACTTGACTTTGTATCATATTGGACAGTGTCCATGTATAGTGGAGAGGAGAATGAAAGGAATTTCTTTCCCCCTAATGCTGTAAAAACAGTTGAAACAGGAAAAAAAAAATTGTAAAGGTTTTGATTTATTTAAAAAAAAAAAAAAAAAATCG



gi|164395169|gb|DB946138.1|DB946138 DB946138 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_023_M2

TACCAAAGAAGATTTGCCCCA
|||||||||||||||||||||
ATGGTTTCTTCTAAACGGGGT

EST sequence (targeted fragment marked with yellow):
TCAAAATCATCTCATTAATTATTATTTCCTTTTTGAGCCTCGAAATTCAGAACTTTTATATTGTGTACGGAACTTCAACGGCCTTTGTAGCATGCAATAATTTGCCCTTCGTTGAAACCCATTAACATTTTTACTCAAATATTTCAGGATTATGCAATGGGATTGGGTTTAGCTTATCAATGTAATAAGATGCACACGTGTGATCAATATATATACACATGCATGAAAAAGAAATCATTCAACTAGAACTTACTAACACTATCATAGAATTCCAAGAGAAGCTGAAAATAAAGGCATTGCTTGAGAGAGAATTGTATGGACTGAATTGTGGGGCAAATCTTCTTTGGTAGTAGGCCTACTCTTTGTAGTTGATTACAACATGAGTTGCCAACTGCCAAAGCAAGAATTGCCCAGGAATGCATACCCATAAACATAAGGGTACGTACCCTTAAACTATGTACTGATGAAACAGTGCCCATAAACATAAGGGTGCGTACCCTTAAACTTTGTACTGATGAAACAGTTTCAAGGTTCAATAGTAATTTATAGTTCAGT