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mature miRNA: GTGATGAGGAGTTGGAACTTG
species: Centaurea solstitialis
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|124689802|gb|EH784538.1|EH784538 CNSS23774.b1_L16.ab1 CNS(LMS) yellow starthistle Centaurea solstitialis cDNA clone CNSS23774

GTGATGAGGAGTTGGAACTTG
|||||||||||| .|| |||
CACTACTCCTCATTCTGTAAC

EST sequence (targeted fragment marked with yellow):
GGCTATTCACTGCCTGCTTTGGATATATTATGCGATGCTCAAGAGCAATGTCTTACTCCTCATCACTATCAACTCAGTTGGTTGCTTCATTGAAACCTTTTACATAATTTTCTTTCTCATCTATGCACCAAAAAAGGCTAGGATGGAGAGTTTGAAGCTAATTGTGTTGCTAATAGTTGTTGGGTTCGGATTGATTGTTGCCTTGACTCAATTCTTTGCAAGTGGAGTTACTCGTGGGGTCATAGTCGGATGGATTTGCCTTGTATTTTCTTTATGTGTTTTTGTAGCACCATTGGGAGTCCTGCGACAAGTGATTAAAACAAAAAGTGTGGAGTACATGCCAATTCTATTATCGGTAGCACTCACCCTTAGCGCTGTCATGTGGTTCTTTTATGGCCTACTTCTTGGTGACTTTAATATTGCGATTCCAAATGTACTTGGATTCATTTTTGGTATCATCCAAATGATACTATACTTTGTGTACAAAAATAAGAAGCCCATCATCGATGAAAAGATTTCCAAATTTGATGAGAAAATTAGTGAAATGGAGGAGCAAAAATTTTCACAATTAAAGGATACAAAAAGCATTGATGTGGTAAAGCTGAAAGGGATGATGCCTGCTGAGATTCTTTCGGGGGTTGGAAAATATGTTGAGCCTCAAGCTGTCCGAAACATGCCAAACCATACCATTGAAGTTGGCGCTTGAAACCCTAANCATTTGATTTTGATTTTCTGTAAAACAAACGATGGGATGAAATTTCCATTCATATATAGCATTTCCATATCTTGACCAAAAAAAAA