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mature miRNA: TATGTTAACGGATTTCATGAA
species: Helianthus tuberosus
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|125442959|gb|EL454655.1|EL454655 CHTM7318.b1_K05.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM7318, m 

TATGTTAACGGATTTCATGAA
||.||.||||||||| | |||
ATGCAGTTGCCTAAATTTCTT

EST sequence (targeted fragment marked with yellow):
GCTTCGGCGGGGACTTCTGAAATTATTCTAACTCAGCGGTTTGAGTTCGAAGCAGATAAAAGATTATGATGACCTTATATTTCTGTCCTGCATTACAGCTTGCAAGACAAGCTTTGTAGTGACTTTGACGATCGTTGACCAGTTATTCTTTAAATCCGTTGACGTATTTGCCCATGGGCAGTTGTGAAGAAAAGACTTGTAAACCCGAAAAATCATCTTCACCTCCACCTCCACCTGCACCTGAGCATCAGCAGGCCGGTGTACATGCATATCCCGATTGGACTACCATGCAGGCTTATTATGGGCCTAGGATGGCGGTGCCACCATATTTCAACTCAGCTGTTGCATCTGGTCATGCCCCTCCACCTTACATGTGGGGACCACCACAGCATATGATGCCACCGTATGCTGCGATGTTTCCACATGGAGGTGTTTATCCACATCCTGGAGTTCCTATTGCGACCCCTATGAGCATTGATTCTCCTGCTAAGTCATCAGGGAACTCTGATCGTGGCTTGATGAAGAAACTGAAAGGATTCGATGGGCTGGCAATGTCAATAGGAAACGGCAATGACGATGGAAATTCACGCAGTGGGGAGACCGAAGGTTCTAGTGAAGGAAGTGATGGCAATACAACTAAGGGGGGTCAAATTAATGGAAAAAGGAGCCGCGAAGATTCACCTAATGCTCCTTCTGTAGTTGGCAAGACTGAGCCATTCAGCGGTCAATTATATCCTACCGAACAAAACGAAGCTTCCAAGAACGTGACCGCACTCACTATAAACCCTTATAAAGCCTCCGATTCTGAGCTGAAGAAATCTCCCACTGCGATGACATCAGCTGCTCTTGCAGTAGTGCCAAGTGAAGCCCTGAGCGAGCGAGAACTAAAAAGGAGAGGAGAAAGCAGTCTAATCGAGAATCTGCCAGGAGGTCTAGATTGA


gi|125426490|gb|EL438462.1|EL438462 CHTM13347.b1_F02.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM13347, 

TATGTTAACGGATTTCATGAA
||||||||||| ..|| |.||
ATACAATTGCCGGGAGAATTT

EST sequence (targeted fragment marked with yellow):
CCAGGGCTCCATTGAAGCTCTATCTTTCCTTTGCTTACGTCATCATCTCCCCTCTCTGCTGGTGTTCTCAGCAGGGTCTTCCTCAATCTTTTCCAACTGTTTCGATGCGTTTGAGGCCAAAACGGACTTGGTCTGGAGTAGAGTGTTTTGGTGGGTTTCACATAAACAGATTATTTCATCTTTTTTTGTTTTTAAGAGGGCCGTTAACATATAAACCCTAATAAGTTTTAGGTTATTTTATTTAAAAGTTTTGTGTTTTTGGTTGTTTTTATAAAATAAAATTATTATTTGAGGCTGTAACTGTACAGCCGCCGGAGTTTTTGAAAACAAAAGAAGTCTTCATGGGACAAAACAAACCCATGAGGAAGATCCGGGTCATCTGTTACGACCCGGATCTAACCGACTCCTCCGACGACGAACCTGATAACAAACCATACGGGTCAAAACGGATTGTTCGGGAAATCAAGATTCCGGTGGCGGCTGACGGTGGTTTTGAGACGGCGGCGGTGGTTACAAGTTCGGTTCAAGATAGTAGTAATAATAACGGTGAGAAAACATGTGTGAAGCGAAAGAGGGTTTTGACTAAATCTCCGGGTCAAAGTCAAAGGGTTTCCGGGTCGAAGTTTAGGGGGGTCCGGCAACGGAAATGGGGCAAATGGGCCGCGGAGATCCGTGACCCGTTTAAGCAGAGACGGGTCTGGCTTGGGACTTATAATACTGCTGAAGAAGCTTCTAGAGCTTATGAAGAGAAAAAACTTGAGTTTGAAACCCTAGCTGAAGCTTCAAAGGGGGGGTCAAATCATGTTAAAACCGGACCCGGAA


gi|125436716|gb|EL448413.1|EL448413 CHTM27102.b1_K08.ab1 CHT(LMS) Jerusalem artichoke Helianthus tuberosus cDNA clone CHTM27102, 

TATGTTAACGGATTTCATGAA
||.||.||||||||| | |||
ATGCAGTTGCCTAAATTTCTT

EST sequence (targeted fragment marked with yellow):
GCCTTCTGAAATATTCTACTCAGTTAGTGGTTTGAGTTCGAAGCAGATAAAAGATTATGATGACCTTATATTTCTGTCCTGCATTGCAGCTTGCAAGACAAGCTTTGTAGTGACTTTGACGATCGTTGACTAGTTATTCTTTAAATCCGTTGACGTATTTGCCCATGGGCAGTTGTGAAGAAAAGACTTGTAAACCCGAAAAATCATCTTCACCTCCACCTCCACCTCCACCTGCACCTGAGCATCAGCAGGCCGGTGTACATGCATATCCCGATTGGGCTACCATGCAGGCTTATTATGGGCCTAGGATGGCGGTGCCACCATATTTCAACTCAGCTGTTGCATCTGGTCATGCCCCTCCACCTTACATGTGGGGACCACCACAGCATATGATGCCACCGTATGCTGCAATGTTTCCGCATGGAGGTGTTTATCCACATCCTGGAGTTCCTATTGCGACCCCCATGAGCATTGATTCTCCTGCTAAGTCATCAGGGAACTCTGATCGTGGCTTGATGAAGAAACTGAAAGGATTCGATGGGCTGGCAATGTCAATAGGAAACGGCAATGACGTTGGAAATTCACACAGTGGGGAGACCGAAGGTTCTAGTGAAGGAAGTGATGGCAATACAACTAAGGGGGGTCAAATTAATGGAAAAAGGAGCCGCGAAGATTCACCTAATGCTCCTGCTGTAGTTGGCAAGACTGAGCCATTCAGCGGTCAATTATATCCTACCGAACAAAACGAAGCTTCCAAGAACGTGACCGCACTC