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mature miRNA: TGCACTGCCTCTTCCCTGGCT
species: Manihot esculenta
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|182639708|gb|FF535913.1|FF535913 CASRI77TF CASR Manihot esculenta cDNA 5', mRNA sequence

TGCACTGCCTCTTCCCTGGCT
||||||||||||||||| . .
ACGTGACGGAGAAGGGAGTCG

EST sequence (targeted fragment marked with yellow):
GGGCACACTCTCTCTTGTATCCTCTTTCTCTCTTTCTCTGTGTGTTTTTGCTTCTTTTGAAGAAATATGGCTGAGGGAAGAGGCAGTGCAGTGGTGGGCATGGTGGCCCTGTGGTTGTTGCTGCTCCATTTTGAGATGGGTAGTGCAGCAACTTATACTGTTGGAGGTTCTAGTGGTTGGACCTTTAACGTTTCTGGCTGGCCTAAAGGAAAACGCTTTAGAGCTGGCGATATAATTGTGTTCAACTATAGTCCTTCAGCACACAACGTAGTGGCAGTGGGGAGGTCTGGATACAGTTCATGCAAAGCTCCAAAAGGTGCAAAAGTTTATACATCAGGCAAGGATAGGATCAAGCTTGTGAAGGGCCAAAACTTCTTTCTCTGCAGCTTTGCTGGCCACTGTCAGGCTGGAATGAAAATAGCCATAACTGCTGTCTGATAAATCTAGCTACCTTGTTAAAAATTTAGAAATATGAGAAATTTTAATTTCTCTTTCACTTTTCTGTATGTTTAATTTTAGGTTTTTGTGTTAAAAATTGTCACTCATGTATGGGTCTGACACAGACTCAATCTGAGTGCTGGTGTGTCTTG



gi|119013381|gb|DV454751.1|DV454751 CV02016B2D11.f1 CV02-normalized library Manihot esculenta cDNA clone CV02016B2D11.f1, mRNA s

TGCACTGCCTCTTCCCTGGCT
|||| ||||||||| |.|||
ACGTCACGGAGAAGATATCGA

EST sequence (targeted fragment marked with yellow):
CGATCCAATTGCACAGGCAATAGGAGCTATTAGTTGCATGATCAGGAAACCAACTGATGGGAAGCTAATGGGATATAATGTTGACTATCTTGGAGCCATAGGAGCTATAGAAGAGGCACTGCAAGGATCAAATGGTAGCCCTGCTTCTGGTTCCCCCTTGGCTGGTAAACTCTTTGTTGTCATGGGAGCTGGTGGAGCTGGAAAGGCCCTTGCTTATGGTGGTTACGAGAAAGGAGCAAGAGTAGTTGTTGCTAATCGCACTTACGAGAAAGCCAAAGAACTTGCTAGTAAAGTTGGAGGAGAAGCAATGACTCTAGCTGAGTTGAAAGAGTTTCATCCAGAAGAGGGAATGATCCTTGCAAATACCACTTCTGTTGGAATGAAACCGAGAATCCACGATACCCCACTTCCTAAGGAAGCTCTGAAGCACTACTCCTTGGTCTTTGATGCAATTTACACACCAAAAATGACCAGGCTCTTAAGAGAAGCACAGGAATCTGGAGCCACTATTGTTTACGGAACCGAGATGTTCATCAATCAAGCATTTGTACAGTTTGAAAGATTCACTGGTTTTTCTGCACCAAAACAATTAATTAGGGATGTGTTGGCAAGAAACACATGAGAAGAAAATTTCCTGAGTGAATCTCTTCTTGCAGCTGAATTTAAATTTTGTTTTTCCAGTATATTGCNNTCAGCAGCTATTCAATTGTTTTCTTGTATGAATAAACTTAATGTTGA



gi|182383173|gb|FF380656.1|FF380656 CASLB77TF CASL Manihot esculenta cDNA 5', mRNA sequence

TGCACTGCCTCTTCCCTGGCT
||| ||||||||| | ||||.
ACGAGACGGAGAAAGAACCGG

EST sequence (targeted fragment marked with yellow):
GGGGGCCAAGAAAGAGGCAGAGCAGAAAGCTCATGCTGAGAAGGCAGCAGGAGCAGAGTCTTCATCTGCTGCATCAAATACTGGAAAAGAGAAATCATCAAGTGAGAAGGATGGGGATTCCATGCAGGTTGATAGCCAGCCAGAGAAGAAGGCAGAGCCTGAACCTTCTTTTGAGATTTTGACCAACCCAGCAAGGGTTGTTCCTGCCCAGGAGAAGTTCATTAAATTTATGGAAGATAGCAGATACGCACCAGTGAAGTTAGCACCCTCAGGGTTTGTCCTCCTGAGAGACCTGAAACCAACTGAGCCAGAGGTGCTATCTCTTACAGATGCACCATCATCTACTGTGTCACCTGCAGGCGGTTCAGCTACAGGACAGCAAGGTTCTGCATCAGCCATGGCTGTTGATGAGGAGCCTCAGCCGCCTCAGCCCTTTGAGTACACAACATAATTCTGGTCATGGCGATTTTCACATTGGTCCTCAGTTAAAATCATGTTGCTTGTAACTGGCAGAGAAAATTAAATGCGGCTCAAATGATCACTTCAATCTTGCATGCATGGTGATGGCCAACATAAGCAGGTTTGCTTGTTTCAAGGCTTTCTTATCAGCAGAGTAAACCAATTTTTTTTAGGTACTGGATAGATACATGATATCAACATATA



gi|164383326|gb|DB926840.1|DB926840 DB926840 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_019_N1

TGCACTGCCTCTTCCCTGGCT
||||||||||||||||| . .
ACGTGACGGAGAAGGGAGTCG

EST sequence (targeted fragment marked with yellow):
GGCACACTCTCTCTTGTATCCTCTTTCTCTCTTTCTCTGTGTGTTTTTGCTTCTTTTGAAGAAATATGGCTGAGGGAAGAGGCAGTGCAGTGGTGGGCATGGTGGTCCTGTGGTTGTTGCTGCTCCATTTTGAGATGGGTAGTGCAGCAACTTATACTGTTGGAGGTTCTAGTGGTTGGACCTTTAACGTTTCTGGCTGGCCTAAAGGAAAACGCTTTAGAGCTGGCGATATAATTGTGTTCAACTATAGTCCTTCAGCACACAACGTAGTGGCAGTGGGGAGGTCTGGATACAGTTCATGCAAAGCTCCAAAAGGTGCAAAAGTTTATACATCAGGCAAGGATAGGATCAAGCTTGTGAAGGGCCAAAACTTCTTTCTCTGCAGCTTTGCTGGCCACTGTCAGGCTGGAATGAAAATAGCCATAACTGCTGTCTGATAAATCTAGCTACCTTGTTAAAAATTTAGAAATATGAGAAATTTTAATTTCTCTTTCACTTTTCTGTATGTTTAATTTTAGGTTTTTGTGTTAAAAATTGTCACTCATGTATGGGTCTGACACAGACTCAATCTGAGTGCTGGTGTGTCTTGC



gi|164381345|gb|DB921820.1|DB921820 DB921820 full-length enriched cassava cDNA library Manihot esculenta cDNA clone CAS01_005_P1

TGCACTGCCTCTTCCCTGGCT
.|||||||||||||| .. ||
GCGTGACGGAGAAGGAGTAGA

EST sequence (targeted fragment marked with yellow):
GGGCGTTCAAAATCTTTGATTTGCTTATTTGTTTGAAAGTTAATGAAATCAGAGACCGAGAATGTTTCTGAGAAGCGGTTCGACGATGAGAATGATACGTAAACGGTCGGCGGTGAAAAGCTTAGTAGCTATAATTCCCTTCTTCGATGTCAATATTTGCTTGGCTTTATCACTTGACAACACTAAACTGGAGGAGCTTCATTGTGGAAGCACACAATTGAGAGTGGCGGATCTGAGATTCAGATGAGGAAGAGGCAGTGCGAGCATTTGGGTTTTCTTTTGTTTTTGGAGGACTGGTCAGCTTGCAAACCCGATCAAGAAAGTTTGGGCATAGATTTATAAAAATAAAAAGGGAAAAATTACTATCAACCCTTTAAATTTTAAGAAATTTACTAATACATTATTATTTTAAAATTATTTTATTAAAATATTTTTTATTTTATAAAATCAAAATTTTAGTTTTTTAATCAATTTATTTTACATTAAAAGAAATTAAATAATATAAATATTTCAAATTATATAACTAAATAACACATATATTAAAATTATAAAA



gi|182382656|gb|FF380140.1|FF380140 CASL655TF CASL Manihot esculenta cDNA 5', mRNA sequence

TGCACTGCCTCTTCCCTGGCT
||||||||||||||||| . .
ACGTGACGGAGAAGGGAGTCG

EST sequence (targeted fragment marked with yellow):
GGGCTCCTCACTGCACACTCTCTCTTGTATCCTCTCTCTCTCTCTCTCTCTTTTTCTGTGTGTTTTTGCTTCTTTTGAAGAAATATGGCTGAGGGAAGAGGCAGTGCAGTGGTGGGCATGGTGGTCCTATGGTTGTTGCTGCTCCATTTTGAGATGGGTAGTGCAGCAACTTATACTGTTGGAGGTTCTAGTGGTTGGACCTTTAACGTTTCTGGCTGGCCTAAAGGAAAACGCTTTAGAGCTGGCGATATAATTGTGTTCAACTATAGTCCTTCAGCACACAACGTAGTGGCAGTGGGGAGGTCTGGATACAGTTCATGCAAAGCTCCAAAAGGTGCAAAAGTTTATACATCAGGCAAGGATAGGATCAAGCTTGTGAAGGGCCAAAACTTCTTTCTCTGCAGCTTTGCTGGCCACTGTCAGGCTGGAATGAAAATAGCCATAACTGCTGTCTGATAAATCTAGCTACCTTGTTAAAAATTTAGAAATATGAGAAATTTTAATTTCTCTTTCACTTTTCTGTATGTTTAATTTTAGGTTTTTGTGTTAAAAATTGTCACTCATGTATGGGTCTGACACAGACTCAATCTGAGTGCTGGTGTGTCTT