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mature miRNA: TCGGCCTTGAATGTTAGGAGAA
species: Pinus taeda
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|5903525|gb|AW042996.1|AW042996 ST28A02 Pine TriplEx shoot tip library Pinus taeda cDNA clone ST28A02, mRNA sequence

TCGGCCTTGAATGTTAGGAGAA
||||||||.||||.|||||||
AGCCGGAATTTACGATCCTCTC

EST sequence (targeted fragment marked with yellow):
GGAGGCACGTATATGGGGAGTCTTTGAGATTAGATTGGAAGAACAAAAATGAAAATGTGTTTGAAAAGGCAGGGTAGCGAAGACCGGTTACCATTATAGTAAGCATCAGTTTGGATCAGGAATATAGTAAATTATAGGTTGTGGTCAATAAGATAAGATGAGATAAATATATTAATGTTCGACAAATGACCACTGAAGTAGGTCAATGGGACTCTCAGGTATGATGATGTGTAAACAAAGTGGGAGTGCTAAGGTAGCTCCTTTATAAAAGGCAAGAAGTAGAAAATGAAAACATGGTAAGGTCCAATCTAAAACCTAGAGAGTTGACTTAAAAAATGAAGATGTGTTCTAGGAGAAAGATGTACAAGATGTGTGTCACAAATGAATGGCACCAGAGGTAGGAGGTTGCTCTGGTTCACAAGAACCGTCTCTCCTAGCATTTAAGGCCGAATG



gi|74155597|gb|DT624330.1|DT624330 EST1158605 Sequencing ESTs from loblolly pine embryos Pinus taeda cDNA clone PIMAD46 5' end,

TCGGCCTTGAATGTTAGGAGAA
|||||||.||||..| ..||||
AGCCGGAGCTTATGAGTTTCTT

EST sequence (targeted fragment marked with yellow):
CGTTTCTGAAGTGACAGAAATTGGGAAATTAATGACTGTTTTTTGTCCGTTATTTGACGTGAAGTATTAGGGTTCGTTAATGGCGAACGCTGCGAGTTTCGATTCTCAGACCTCTAGACTAGCGATGGATAACAGATCGGTACTGAAGCCCGAGCCTCTGGTGAAAATGAGTGTCAATATTTCTGGTTCTGGTGACGGCGGATTCACCGTCAATAGACAGGGGCAGATTTCCGTCAAGAAAGCCCGCGCTGTTCACATCCAGGTTATGAGAATCCGCGAAGAAGATGAGCATCTTGGTGAGGATTTAATGGAGGGCGTGAATCCGAAGGACAGGTTTGTGTTTTTCCCGATCGCGTCGCAGATGAAGGACATGTTCTTTGAGTATTCGAGGCCGACTTTCCCGTCGCCGCTCGGCATGAACGCTGGAGTTCGGTCGCTCTGAGAAGATTCTGACACTCATCTGAGTTTTGAGAAGGTTTTGTGTGTAGATATTTGGATCTGAGCCATGAGGCATTTGAGGCCTTCTGTATATTAATTGTTGTGAAGTTATTTCTGTCTGTAAATGCTTTCCCTGGCAGTTTTGTTTAAAACCTGCTGTGTTGGAAATGGGGCAGTTGCTTTCTGCCAGAATAATGTAAATGCAATACAGTGATCAT



gi|21821417|gb|BQ696101.1|BQ696101 NXPV_036_D04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda cDNA clone NXPV_036_D04 5'

TCGGCCTTGAATGTTAGGAGAA
|||||||.||||..| ..||||
AGCCGGAGCTTATGAGTTTCTT

EST sequence (targeted fragment marked with yellow):
CGCAGATGAAGGACATGTTCTTTGAGTATTCGAGGCCGACTTTCCCGTCGCCGCTCGGCATGAACGCTGGAGTTCGGTCGCTCTGAGAAGATTCTGACACTCATCTGAGTTTTGAGAAGGTTTTGTGTGTAGATATTTGGATCTGAGCCATGAGGCATTTGAGGCCTTCTGTATATTAATTGTTGTGAAGTTATTTCTGTCTGTAAATGCTTTCCCTGGCAGTTTTGTTTAAAACCTGCTGTGTTGGAAATGGGGCAGTTGCTTTCTGCCAGAATAATGTAAATGCAATACAGTGATC



gi|67050061|gb|DR072263.1|DR072263 RTDK1_25_D02.b1_A029 Roots, dark Pinus taeda cDNA clone RTDK1_25_D02_A029 3', mRNA sequence

TCGGCCTTGAATGTTAGGAGAA
|||.||||||||| ||.||||
AGCTGGAACTTACCATTCTCTC

EST sequence (targeted fragment marked with yellow):
TTCCCTCGTGCCGAATTCGGCACGAGGCCACCATCAAAATATAGAATTACTAGACACTTGAAGAAATTCCATCACTAAACTACTCATCATTTGAGGGATTGTAATCTTAATCTCTCCATTTGATAAAGAGGTGCTCCAAAGAGGTATGAACACAACATAAAAAATGATATGTTTATTAATGTTCAACAAATGACTGAAATTTAGTCCACAGGACTATTCAATACAAAAGTGTCTGAGTGTTAAGGCTACTCCTATAAAAAGCAAAGCAAAACAAAGCTATTGTATAAAACTATACTCTGTTAGTGGAAAGCCAAAGTGATAAAATGTTTTGGTTAATCAAAAAGAAGAAAAGAGTCTCATCTCTCCTAGTGTTTGCCACGAGAAGGACAAGGGGGGTTGTCTCCCTAAGTGTTCGCTGTCAAATGTGTCGCTCTCTTACCATTCAAGGTCGAAGAGGTGGTTTGTCATTGGGATTGGGGGAAGGCATGTTGTTTGCTAGGTTGACGTANGATACCTTGAGATCTAAAATGGTACCTTGTGATCTAAAATGGTGGCTCCATAATGCCTTGTTGGAGCTAGGGGATCGATTTCACCTTGGAGTTTGATGGATGCAAACCTTGAAATGGTATCGCTCTTTGCTTGTCTCTTTG