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mature miRNA: TAGCCAAGAATGGCTTGCCTA
species: Sorghum bicolor
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|18067500|gb|BM328363.1|BM328363 PIC1_28_D07.g1_A002 Pathogen-infected compatible 1 (PIC1) Sorghum bicolor cDNA, mRNA sequence

TAGCCAAGAATGGCTTGCCTA
|||||||||||. ||||||
TTCGGTTCTTACTTAACGGAC

EST sequence (targeted fragment marked with yellow):
AAAAAGGTGGTCAAAGCCAGAAAGCCATACCTTCATGAGTCTCGTCATCAGCACGCGATGAGGAGGGCAAGAGGAAATGGGGGACGCTTCCTAAACACAAAGAAAAGTGACAATGGTACTCCCAATGGTAGAGCTGAGCCTAAGAAAGGAGATGGTAACTCCGAGCATCTCCATGTCCCTCCTGACTTACTACAGCTACGACAGAACGGGGCTTGAAGTAGCGTTCTGGCTCTGGCATCCTAGAACAGCAGTTTCTGCCCACAGGCATAGGCATTTGAAACCGGACTCGTAGCTCCACAGTATGCGCAGCCATATCTGCGGTAACGCTCGTTCACCTGAATGAGCTTTGTACCGAGGTAGGCATGCAATGCAATCTGGGCGCAGGAGTCCATCAGGTGTCCAATGCCATATGGGTGTATGCTGTTTCAGGCAATTCATTCTTGGCTTTCACGTTCCACCACCCATGTGTAGCGCTAGTGTGTTGTAAGTGAAACTAAGCTTGTGCTCTGTATTGGGCCGTTCGGCGAAACTGTGAAACATCTGTATAATTAAGATCGAACTCTGGATTAACTCGGTAGTGCTTTGTCTGGTTTTTCTTTT



gi|6673990|gb|AW284251.1|AW284251 LG1_268_F03.g1_A002 Light Grown 1 (LG1) Sorghum bicolor cDNA, mRNA sequence

TAGCCAAGAATGGCTTGCCTA
.|||||||||||. |||||||
GTCGGTTCTTACTAAACGGAT

EST sequence (targeted fragment marked with yellow):
CCAAATGCTAGGAGCTGGTGACTGCTGGCAGTTCCCAGCTCCAGGGTCCGGGATTGCCCCTCCCATCGTTTGGCTCCCGGTAGGCAAATCATTCTTGGCTGAAGGCATTGTTGAGCGTTCCACTCATGCCGCCTAGTTCTCCTAGAAACTCTGAAACCTAGTTTCTGGGATCCTGTGGTGAGTGGAACATTCCTTTGCCTCAAAAAACAAAACAAAAAAAAAGTTGGTTGTACTATCGACCAACTTCTAAATGGTTTCACCTTATTAGCTTTCAGAATAATAAAGCCTGATACTTCGGACTCGTCTGAAGTTTCAGAGGTACTGGAATTGTGCTATGTGTGAGTCTGTTAAAGCGTCAAGAACTACGGATTTGTTGGTGATGAATTGTAGTGTGCTTGAAGCTTATGTGGACTTGATTCGTACTTTTGTGGTGTGCTGAAAACTTAAACTCGGTAATGTAATGAAACTCGGGCTTGGTTTGT



gi|14513763|gb|BI075106.1|BI075106 IP1_19_F11.g1_A002 Immature pannicle 1 (IP1) Sorghum bicolor cDNA, mRNA sequence

TAGCCAAGAATGGCTTGCCTA
.|||||||||||. |||||||
GTCGGTTCTTACTAAACGGAT

EST sequence (targeted fragment marked with yellow):
CTCCACGACCACCAGGGCTCAGCAACATTAGCAACCCGCACTTCCACTCCCAGAGTAGCGTCTCCAGCTTGTCTGGCTCCGATGTGTCAAACATCTACGGCGGCTTGGAGCAGCCCCCTCGGGCGCCACCCTTCTTCACCGTGCTGCCACCCATCATGGACGGCGACCATGGTGGCGTCACCCACATCTCTTCCTTCAAGTGGGCAGCCAGCGACAGCTGCTGCGAGCTCCTCAAGGCATGAACCGAGGAGGAGGGGATGGCTACTCAGACAAACGGCCTTCTCCCCGATGGCTGGTAGTCTGTAGGCAAATCATTCTTGGCTGTTCTGCATTGGGGTGCAACCTCCACATCATCTACCTACCTACCCACCCCCTGTCCCTGAAATTCCACAGTGCTTTGGGTTACAGGGGTCTTGTGTGATGATGTGGCTCCCCCACATGCCTTTGCTGTAACATATTGTACCCAAACCACTGCTACTTGGTACTTCTCGGTATCATTGTCTCAGTATGGATTCTCCGTTGTCACAGTGTTTGAATAATTGAGGCGTCAGACTTCAGGGTT



gi|57809436|gb|CX610716.1|CX610716 ANR1_20_H10.b1_A002 Anaerobic roots Sorghum bicolor cDNA clone ANR1_20_H10_A002 3', mRNA sequ

TAGCCAAGAATGGCTTGCCTA
||.|||||||||. ||||||
ATTGGTTCTTACTCAACGGAG

EST sequence (targeted fragment marked with yellow):
TTGATACTTCAGCTTCCTTTGAAGAAACTGATATTTCAAACTTGATTATCAACATGACTAATAAGTTTCGACGATAGCTTTACATTTAAAAGCCTCGTTTACTCCTAACAACAAAGTTTTGGTGGAGTACAACTATAGGTGAATTTCTCTGAACTCTTCTTTATATACAACACCATCAAGGTGGAAAACGGATGCTACAAGGCTGTGGAGATCTCTATGAGTTGGAGGTATTCCACAGTATAAAATAGCATATTAGTTTTTCAAAATTTTCAAATAGATAAGAGATGCCTCAACTGAAGCAAGTGCTCCTCATTTGAGATGTTGAGCGAGAGGCGCAGGTTTGTCAGTAGTGACTCTTGCTCCCATGTCAGGGGTCCTGCAGCATGGAAAGCTCTGACAGTGGATTGGTATGCTTGCAGCTCCGGCTCATGGACATTCAGAAATAAACCAGAGCCATAGGTGTTCTTAATCTCCTGCCCAGATTGACATGCAGACATAACATCATCAGGGAAACTATTGCCGATTCCAACTGATTGTTGGTTATCACTGGTACTGTACTCTGAATAATTTGCGCTGCAACTAGCCACTGAGCACTCATTCTCTTCTCTGACGCGCAAAGGTTTCTCAGACAAAGGAAGAAATTTGCAAACTGGTTTTATATCAGAAATGTTCTTGGTAAGATCATTGTATCTGCTACTTAAAGACCTATGAAAGTGATCTCCATCGATGGCATTCCTAGCAGACACCTTCAGAGGCAACTCATTCTTGGTTAACTGACGAAATTCATATGCAGCTACTTTCCGTTCCTTGCCTGTTCTTCTAACTAAATCATTAGGTGGAATCCTACTTGCTTC