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mature miRNA: TGACAACGAGAGAGAGCACGC
species: Zamia vazquezii
database: miRNEST
The numbers on the right side of the alignments show a score calculated for them.
When calculating the score, a mismatch is given a score of 1, bulge: 2, and wobble: 0.5.

Predicted miRNA targets
gi|166947395|gb|FD775473.1|FD775473 Zfi03b_24_G08_C018.g1 Second Zamia male gametophyte library Zamia vazquezii cDNA clone 122Oo

TGACAACGAGA-GAGAGCACGC
|||||||| || ||||.|||||
ACTGTTGCCCTGCTCTTGTGCG

EST sequence (targeted fragment marked with yellow):
AGTGCTCCGCAAAACTGTAGCGAACAACAACGGCCCTTCGATTCACCGTGTTCTCTCGTCGCTGCTAAGGCTGCGAGCTGCTGATGCTGCTAGAGCCGAAATTCTCCGAGTCACTTCGCATTCGACCGTGCAGGGTTTATGGCTGTGCAGATACCGCGCTGTTGACGACGAGAGAGAGCACGCTTGTAGACAATAATATGATTTTGGACGGTTATTACAACGTATTTCTGTGTACATGCGTGTTCTCGTCCCGTTGTCAGCAGTAATGAACAGGAGTCCAATTTTGGAGAAAGCCTCTGAAGGCAGCAACAGAGCGAGAATGCCAAAATTAATGGTCAAAATAGATATCCGCCTGCTGCTCAGAGATCGCCAAAGGCGGCGAGCTGGACTTCATTGTCTCGGGAGGTTCTACCGGCGTTGGAATATGGAAACTTGGTATGTGTTAAGTAGATACAGCGCCTGTGACAGGCACTGCAGGAACGCTGCCTATTTAAAAATAGTTGCCCATCACCAATTTATTCATCGTGAAAGAATTTTTATCCTTTCATATTCCCGATTTGATTGAATCGGTCCGTATTGATCTACAGTTTTCAAATTTTTTTTCATTTGAGTTGAGAGAGGCGTACCTTCTCCTTCCAGGGATCGACTCACCGC



gi|166947347|gb|FD775425.1|FD775425 Zfi03b_23_G08_C018.b1 Second Zamia male gametophyte library Zamia vazquezii cDNA clone 122Oo

TGACAACGAGAGAGAGCACGC
|||| ||||||||||||||||
ACTGCTGCTCTCTCTCGTGCG

EST sequence (targeted fragment marked with yellow):
CCCCTCGAGTTTTTTTTTTTTTATAGACCAAGAGTATTAATTAAATATAAAAATATATAATTAGCATAATTATTCGATAGGAACGAGAGCCAGCTTGGCGGCGGTGAGTCGATCCCTGAAAGGAGAAGGTACCCCTCTCTCAACTCAAATGAAAAAAAATTTGAAAACTGTAAATCAATACGGACCGATTCAATCAAATCGGGAATATGAAAGGATAAAAATTCTTTCACGATGAATAAATTGGTGATGGGCAACTATTTTTAAATAGGCAGCGTTCCTGCAGTGCCTGTCACAGGCGCTGTATCTACTTAACACATACCAAGTTTCCATATTCCAACGCCGGTAAAACCTCCCGAGACAATGAAGTCCAGCTCGCCGCCTTTGGCGATCTCTGAGCAGCAGGCGGATATCTATTTTGACCATTAATTTTGGCATTCTCGCTCTGTTGCTGCCTTCAGAGGCTTTCTCCAAAATTGGACTCCTGTTCATTACTGCTGACAACGGGACGAGAACACGCATGTACACAAAAATACGTTGTAATAACCGTCCAAAATCATATTATTGTCTACAAGCGTGCTCTCTCTCGTCGTCAACAGCGCGGTATCTGCACAGCCATAAACCCTGCACGGTCGAATGCGAAG