RetrogeneDB ID: retro_cjac_3631

Retrocopy location

• Organism: Marmoset (Callithrix jacchus)
• Coordinates: ACFV01190418.1:3233..3975(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: PPP1R35
• Ensembl ID: ENSCJAG00000015345
• Aliases: None
• Description: protein phosphatase 1, regulatory subunit 35 [Source:HGNC Symbol;Acc:28320]

Sequence

>retro_cjac_3631
ATGATGGGGTGTGGGGACCCAGAGTTGGAGTCGGGGGACGGGGAAGAAGCCGCGGTGGTCCCAGGGCCACCCCCGGAGCC
CCGAGTCCCGGAACCCCGACCCCCGGTGCCCGAGCCCAGCCTGGACTTGAGCCTGAGTCCATGCTCTGGCATCCCTGAGC
CACAGCACGGCAGCCCTGGGCGGCGGAAGAAGGGGGCGGAGCGGCAGGGCGCCGGTCGGCAGCGCGGCAGGTTGGTTTCC
GCCTGGCACCACCCTCCCCGGTGTAGTCCGAGCCGCAGCCCGCTGCGCTGCAGGAGCTAGAGATGGCCACGCTGCAGAGC
AACCTGGCCTTGGGGTCTGGAGCTGAGAGCCGCAGCCGTCCGGCAAAAGCCGTGGAAGAACAGCTGAAAAAGTCGTTCCA
GATCCACTGTGGCCCGGAGGAGAGTGTGTGCGAGGGGCCGAACGTGCCGCTCTCCAAGAGGCTCTTCCGAGACCTGGTGA
GCCTGCAGGTGCTGGAGGAACCGGTTCTGAACGCTGCGCTCGGGGAGAAATTGGCTCTCCTGCCGCCGCAGGCTCAACCC
AGCCCCCAAAGGAGCCACCTGGGCTGGGGCCAGACATGACCATCTTGTGTGACCCAGAAACACTATTTTACGAATCTCCA
CACCTGACCCTGGATGATCTGACCCCTCTCCGGCTTCAACTCCGGCCCCATCCTTCAGAGGACACCTTCCTTATGCACGG
GACACTGAGGCGTTGGGAAGCG

Alignment

ORF - retro_cjac_3631 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 78.74 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 1
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  MIGCGDSELESVDGEEAAAVPGPPPEPRVPEPRAPVPEPGLDLSLSPQPDSPKPPHDRPGRRKERAERRG
          M.GCGD.ELES.DGEEAA.VPGPPPEPRVPEPR.PVPEP.LDLSLSP....P.P.H..PGRRK..AER.G
Retrocopy MMGCGDPELESGDGEEAAVVPGPPPEPRVPEPRPPVPEPSLDLSLSPCSGIPEPQHGSPGRRKKGAERQG

Parental  AGRQR-RQVRFRLAPPSPVRSEPQPAASQELEMPALQSSLALGLELRAAAGSHFDAAKAVEEQLRKSFQI
          AGRQR.RQV.FRLAPPSPV.SEPQPAA.QELEM..LQS.LALG......A.S....AKAVEEQL.KSFQI
Retrocopy AGRQR<RQVGFRLAPPSPV*SEPQPAALQELEMATLQSNLALG----SGAESRSRPAKAVEEQLKKSFQI

Parental  RCGLEESVSEGLNVPRSRRLFRDLVSLQVPEEQVLNAALREKLALLPPQAR-APPPKEPPGPGPDMTILC
          .CG.EESV.EG.NVP.S.RLFRDLVSLQV.EE.VLNAAL.EKLALLPPQA....PPKEPPG.GPDMTILC
Retrocopy HCGPEESVCEGPNVPLSKRLFRDLVSLQVLEEPVLNAALGEKLALLPPQAQ<TQPPKEPPGLGPDMTILC

Parental  DPETLFYESPHLTLDGLPPLRLQLRPRPSEDTFLMHRTLRRWEA
          DPETLFYESPHLTLD.L.PLRLQLRP.PSEDTFLMH.TLRRWEA
Retrocopy DPETLFYESPHLTLDDLTPLRLQLRPHPSEDTFLMHGTLRRWEA

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP051959_colon	0.00 RPM	2.51 RPM
SRP051959_heart	0.72 RPM	1.81 RPM
SRP051959_kidney	0.13 RPM	2.82 RPM
SRP051959_liver	0.02 RPM	2.84 RPM
SRP051959_lung	0.08 RPM	2.96 RPM
SRP051959_lymph_node	0.00 RPM	2.79 RPM
SRP051959_skeletal_muscle	0.02 RPM	1.70 RPM
SRP051959_spleen	0.02 RPM	2.16 RPM

ChIP-Seq

Callithrix jacchus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cjac_3631 retrocopy.

TSS

Callithrix jacchus was not studied using FANTOM5 data.

Lab

retro_cjac_3631 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cjac_3631 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 1 parental gene, and 5 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000015345	5 retrocopies	retro_cjac_3472, retro_cjac_3631 , retro_cjac_3808, retro_cjac_3913, retro_cjac_549,