RetrogeneDB ID: retro_dnov_2499

Retrocopy location

• Organism: Armadillo (Dasypus novemcinctus)
• Coordinates: scaffold_8063:41485..42234(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: ZSCAN4
• Ensembl ID: ENSDNOG00000011483
• Aliases: None
• Description: zinc finger and SCAN domain containing 4 [Source:HGNC Symbol;Acc:23709]

Sequence

>retro_dnov_2499
GATCTGACCAAGGATTGCGTGAAGTCACCTGACTTTGTCCATGTCCACACACAGGGACAGAAAATCCTCTTCTTTGAGAA
TATGCCCTTAAGAGAAGTCATATTCATCTCATGGAACAAATACCAGCAGGAACCCTAAGAGGAGAGAGTATGAGAACCCC
CTTCTGAGGTCCCATATGCTATTTTCCTTCAAACAAGATAAGGAAATGAATATAAAGAAGACAACAGTAACATTTCTGTG
AAAACCAATCAAATAAATGACAGTATTACTTGTCGAGGCAATCAGGTACCTTTCCTGGTTATTATTCAGGAAGATAATAA
CCCTAAGGCTGAAGAGTGGAGTGTCTCTTGGGAGAACTTGCACAACTCCAGTAGAGCAGAGATTGGCCCCTCCAGATCCC
AGGAAGGATCCCTGGAAGATCATCTATCAAAATGTCCCTGTGGAGGTGGGACCACAGTTTTTCTCTAGACCAGCTCAAAA
CAGCCCTAAGCCTGCCCCTACCAACCAAAGCAATGATGGATCATCTAGGTGTGGGGTAAATGAAGAAAGAGTCCATAGAC
CTCCTCATATCATACAGATATGAGGACTGCCCCAGAATCTTTAGTATCTCTCTTCGGTTAGAAGCCCACCAGAGAATACA
CAGGAATGAGAGAACATTTGTTTGTGTTTGAGTATCACAAAAATTACCTCCCAGACTTCAAGTCTTCATGTTGTACCAGA
TGAATCACATTGGTGAAGAAGACTTTAAG

Alignment

ORF - retro_dnov_2499 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 59.61 %
• Parental protein coverage: 57.04 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 6

Retrocopy - Parental Gene Alignment:

Parental  ELNDDCMKSPDFVHVHMQGQEALFSENMPLREVIYLMEQMPAGTPRGESM-RTPFQGPQNIPLQTGHGDE
          .L..DC.KSPDFVHVH.QGQ..LF.ENMPLREVI..........P..E.....P...P..I.LQT..G.E
Retrocopy DLTKDCVKSPDFVHVHTQGQKILFFENMPLREVIFISWNKYQQEP*EERV*EPPSEVPYAIFLQTR*GNE

Parental  YKDD-SNIFVKRNQINVNITSQGNQVPYVVIIQEDDSPKPEEGSGSWKNLHNFRRAELGSPTSQEGSL-G
          YK.D.SNI.VK.NQIN..IT..GNQVP..VIIQED..PK.EE.S.SW.NLHN..RAE.G...SQEGSL.G
Retrocopy YKEDNSNISVKTNQINDSITCRGNQVPFLVIIQEDNNPKAEEWSVSWENLHNSSRAEIGPSRSQEGSL<G

Parental  ESS-SQDVPMEVETQFLPRTDQASPKPALTNQSSDGTSRCGINQERVHSPPK-SYKCEDCPRIFRFPSQL
          .SS..Q.VP.EV..QF..R..Q.SPKPA.TNQS.DG.SRCG.N.ERVH.PP..SY..EDCPRIF.....L
Retrocopy RSS<YQNVPVEVGPQFFSRPAQNSPKPAPTNQSNDGSSRCGVNEERVHRPPH>SYRYEDCPRIFSISLRL

Parental  EAHQRRHRNERPFVCT-ECHKGFF-QTSDLHV-HQKIHMAEKPFR
          EAHQR.HRNER.FVC..E.HK....QTS.LHV..Q..H..E..F.
Retrocopy EAHQRIHRNERTFVCV>EYHKNYL>QTSSLHV>YQMNHIGEEDFK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012922_ascending_colon	0.00 RPM	0.00 RPM
SRP012922_cerebellum	0.27 RPM	0.14 RPM
SRP012922_heart	0.00 RPM	0.00 RPM
SRP012922_kidney	0.00 RPM	0.00 RPM
SRP012922_liver	0.15 RPM	0.00 RPM
SRP012922_lung	0.31 RPM	0.00 RPM
SRP012922_quadricep_muscle	0.00 RPM	0.00 RPM
SRP012922_spleen	0.11 RPM	0.00 RPM

ChIP-Seq

Dasypus novemcinctus was not studied using ChIP-Seq data.

EST

Dasypus novemcinctus was not studied using EST data.

TSS

Dasypus novemcinctus was not studied using FANTOM5 data.

Lab

retro_dnov_2499 was not experimentally validated.

Homology

Retrocopy orthology:

Dasypus novemcinctus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 16 retrocopies.

Species	Parental gene accession	Retrocopies number
Cavia porcellus	ENSCPOG00000005419	2 retrocopies	retro_cpor_1470, retro_cpor_285,
Dasypus novemcinctus	ENSDNOG00000011483	11 retrocopies	retro_dnov_1048, retro_dnov_1253, retro_dnov_1287, retro_dnov_1505, retro_dnov_174, retro_dnov_1780, retro_dnov_2499 , retro_dnov_2556, retro_dnov_2666, retro_dnov_859, retro_dnov_964,
Equus caballus	ENSECAG00000015200	1 retrocopy	retro_ecab_78,
Loxodonta africana	ENSLAFG00000003257	1 retrocopy	retro_lafr_171,
Ictidomys tridecemlineatus	ENSSTOG00000023779	1 retrocopy	retro_itri_408,