RetrogeneDB ID: retro_hsap_2783

Retrocopy location

• Organism: Human (Homo sapiens)
• Coordinates: 3:44619413..44621440(-)
• Located in intron of: ENSG00000236869

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MPRIP
• Ensembl ID: ENSG00000133030
• Aliases: MPRIP, M-RIP, MRIP, RHOIP3, RIP3, p116Rip
• Description: myosin phosphatase Rho interacting protein [Source:HGNC Symbol;Acc:30321]

Sequence

>retro_hsap_2783
CCCCCGTCTCCACACCGAAGAGCCAAGTCACTGGACAGGAGGTCCACAGAGCCCTCCGTGACGCCCGACCTGCTGAATTT
CAAGAAAGGCTGGCTGACTAAGCAGTATGAGGACAGCCAGTGGAAGAAACACTGGTTTGTCCTCGCCGATCAAAGCCTGA
GATACTACAGGGATTCAGTGGCTGAGGAGGCAGCCGACTTGGATGGAGAAATTGACTTGTCTGCATGTTACGATGTCACA
GAGTATCCAGTTCAGAGAAACTATGGCTTCCAGATACATACAAAGGAGGGCGAGTTTACCCCGTCGGCCATGACATCTGG
GATTCGGCGGAACTGGATCCAGACCATCATGAAGCATGTGCACCCGACCACCGCCCCGGATGTGACCAGCTGGTTGCCAG
AGGAAAAAAACAAGAGCAGCTGCTCTTTTGAGACCTGCCCGAGGCCTACTGAGAAGCAAGAGGCAGAGCCGGGGGAGCCG
GACCCTGAGCAGAAGAGGAGCCGCACGTGGGAGCGGAGGCGAGAGGGCCTCTCCAAGACCTTTGACTGGGCTGAGTTCCG
TCCCATCCAGCAGGCCCTGAATCAGGAGCGGGTGGGCGGTGTGGGGCCTGCTGACACCCACGAGCCCCTGCGCCCTGAGG
CGGAGCCTGGGGAGCTGGAGCGGGAGCGTGCACGGAGGCGGGAGGAGCGCCGCAAGCACTTCGGGATGCTGGACGCCACA
GGCGGGCCAGGCACTGAGGATGCAGCCCTGCGCATGGAGGTGGACCGGAGCCCAGGGCTGCCTGTGAGCGACCTCAAGAC
GCATAACGTCCACGTGGAGATCGAGCAGCGGTGGCATCAGGTGGAGACCACGCCTCTCCGGGAAGAGAAGCAGGTGCCCA
TCGTCCCCGTCCACCTGTCTTCTGAAGATGGGGGTGACCGGCTCTCCACACACGAGCTGACCTCTCTGCTCAAGAAGGAG
CTGGAGCAGAGCCAGAAGGAGGCCTCAGACCTTCTGGAGCAGAACCGGCTCCTGCAGGACCAGCTGAGGGTGGCCCTGGG
CCGGGAGCAGAGCGCCCGTGAGGGCTACATGCTGCAGGCCACGTGCAAGTGAGGGTTTGCAGCAATGGAAGAAACGCACC
AGAAGAAGATTGAAGATCTCCAGAGGCAGCACCAGCGGGAGCTAGAGAAACTTCGAGAAGAGAAAGACCGACTCCTAGCC
GAGGAGACAGCGGCCACCATCTCAGCCATCGAAGCCATGAAGAACGCCCACCGGGAGGAGATGGAGCGGGAGCTGGAGAA
GAGCCAGCGGTCCCAGATCAGCAGCGTCAACTCGGATGTTGAGGCCCTGCGGCGCCAGTACCTGGAGGAGCTGCAGTCGG
TGCAGCGGGAACTGGAGGTCCTCTCGGAGCAGTACTCGCAGAAGTGCCTGGAGAATGCCCATCTGGCCCAGGCGCTGGAG
GCCGGGCAGCAGGCCCTGCGGCAGTGCCAGCGTGAGAACCAGGAGCTCAATGCCCACAACCAGGAGCTGAACAACCGCCT
GGCTGCAGAGATCACACGGTTGCGGACGCTGCTGACTGGGGACGGCGGTGGGGAGGCCACTGGGTCACCCCTTGCACAGG
GCAAGGATGCCTATGAACTAGAAGTCTTATTGCGGATATAGGAATCGGAAATACAGTACCTGAAACAGGAGATTAGCTCC
CTCAAGGATGAGCTGCAGACAGCACTGTGGGACAAGAAGTACGCAAGTGACACCACAAAGACATCTACACAGAGCTCAGC
ATCGCAAAGGCTAAGGCTGACTGTGACATCAGCAGGTTGAAAGAGCAGCTTAAGGCTGCAACGGAAGCACTGGGGGAAAA
GTCCCCTGACAGTGCCATGGTGTCCGGATATGATATAATGAAATCTAAAAGCAACCCTGACTTCTTGAAGAAAGACAGAT
CCTGTGTCACCTGGCAACTCAGAAACATCAGGTCCAAGAGTCTGAAGGAAGGCCTGACGGTGCAAGAACGGTTGAAGCTC
TTTGAATCCAGGGACTTGAAGAAAGAC

Alignment

ORF - retro_hsap_2783 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 96.01 %
• Parental protein coverage: 65.13 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  PLSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDGQWKKHWFVLADQSLRYYRDSVAEEAADLDGE
          P.SPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYED.QWKKHWFVLADQSLRYYRDSVAEEAADLDGE
Retrocopy PPSPHRRAKSLDRRSTEPSVTPDLLNFKKGWLTKQYEDSQWKKHWFVLADQSLRYYRDSVAEEAADLDGE

Parental  IDLSACYDVTEYPVQRNYGFQIHTKEGEFTLSAMTSGIRRNWIQTIMKHVHPTTAPDVTSSLPEEKNKSS
          IDLSACYDVTEYPVQRNYGFQIHTKEGEFT.SAMTSGIRRNWIQTIMKHVHPTTAPDVTS.LPEEKNKSS
Retrocopy IDLSACYDVTEYPVQRNYGFQIHTKEGEFTPSAMTSGIRRNWIQTIMKHVHPTTAPDVTSWLPEEKNKSS

Parental  CSFETCPRPTEKQEAELGEPDPEQKRSRARERRREGRSKTFDWAEFRPIQQALAQERVGGVGPADTHEPL
          CSFETCPRPTEKQEAE.GEPDPEQKRSR..ERRREG.SKTFDWAEFRPIQQAL.QERVGGVGPADTHEPL
Retrocopy CSFETCPRPTEKQEAEPGEPDPEQKRSRTWERRREGLSKTFDWAEFRPIQQALNQERVGGVGPADTHEPL

Parental  RPEAEPGELERERARRREERRKRFGMLDATDGPGTEDAALRMEVDRSPGLPMSDLKTHNVHVEIEQRWHQ
          RPEAEPGELERERARRREERRK.FGMLDAT.GPGTEDAALRMEVDRSPGLP.SDLKTHNVHVEIEQRWHQ
Retrocopy RPEAEPGELERERARRREERRKHFGMLDATGGPGTEDAALRMEVDRSPGLPVSDLKTHNVHVEIEQRWHQ

Parental  VETTPLREEKQVPIAPVHLSSEDGGDRLSTHELTSLLEKELEQSQKEASDLLEQNRLLQDQLRVALGREQ
          VETTPLREEKQVPI.PVHLSSEDGGDRLSTHELTSLL.KELEQSQKEASDLLEQNRLLQDQLRVALGREQ
Retrocopy VETTPLREEKQVPIVPVHLSSEDGGDRLSTHELTSLLKKELEQSQKEASDLLEQNRLLQDQLRVALGREQ

Parental  SAREGYVLQATCERGFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREE
          SAREGY.LQATC..GFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREE
Retrocopy SAREGYMLQATCK*GFAAMEETHQKKIEDLQRQHQRELEKLREEKDRLLAEETAATISAIEAMKNAHREE

Parental  MERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAERQALRQCQ
          MERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEA..QALRQCQ
Retrocopy MERELEKSQRSQISSVNSDVEALRRQYLEELQSVQRELEVLSEQYSQKCLENAHLAQALEAGQQALRQCQ

Parental  RENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRVKESEIQYLKQEISS
          RENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLR..ESEIQYLKQEISS
Retrocopy RENQELNAHNQELNNRLAAEITRLRTLLTGDGGGEATGSPLAQGKDAYELEVLLRI*ESEIQYLKQEISS

Parental  LKDELQTALRDKKYASD-KYKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSATVSGYDIMKS
          LKDELQTAL.DKKYASD...KDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSA.VSGYDIMKS
Retrocopy LKDELQTALWDKKYASD<HHKDIYTELSIAKAKADCDISRLKEQLKAATEALGEKSPDSAMVSGYDIMKS

Parental  KSNPDFLKKDRSCVTRQLRNIRSKSLKEGLTVQERLKLFESRDLKKD
          KSNPDFLKKDRSCVT.QLRNIRSKSLKEGLTVQERLKLFESRDLKKD
Retrocopy KSNPDFLKKDRSCVTWQLRNIRSKSLKEGLTVQERLKLFESRDLKKD

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
bodymap2_adipose	0.16 RPM	211.52 RPM
bodymap2_adrenal	0.43 RPM	247.58 RPM
bodymap2_brain	0.21 RPM	197.62 RPM
bodymap2_breast	0.23 RPM	193.87 RPM
bodymap2_colon	0.02 RPM	148.89 RPM
bodymap2_heart	0.18 RPM	184.00 RPM
bodymap2_kidney	0.19 RPM	145.03 RPM
bodymap2_liver	0.04 RPM	37.03 RPM
bodymap2_lung	0.30 RPM	228.96 RPM
bodymap2_lymph_node	0.02 RPM	229.15 RPM
bodymap2_ovary	0.64 RPM	218.08 RPM
bodymap2_prostate	0.40 RPM	191.27 RPM
bodymap2_skeletal_muscle	0.04 RPM	127.06 RPM
bodymap2_testis	0.36 RPM	154.26 RPM
bodymap2_thyroid	0.11 RPM	167.37 RPM
bodymap2_white_blood_cells	0.00 RPM	99.94 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_hsap_2783 was not detected

EST

No EST(s) were mapped for retro_hsap_2783 retrocopy.

TSS

No TSS is located nearby retro_hsap_2783 retrocopy 5' end.

Lab

retro_hsap_2783 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_hsap_2783 has 1 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Pan troglodytes	retro_ptro_1884

---

Parental genes homology:

Parental genes homology involve 2 parental genes, and 2 retrocopies.

Species	Parental gene accession	Retrocopies number
Homo sapiens	ENSG00000133030	1 retrocopy	retro_hsap_2783 ,
Pan troglodytes	ENSPTRG00000008824	1 retrocopy	retro_ptro_1884,