RetrogeneDB ID: retro_mmus_694

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 11:16514532..16515978(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSMUSG00000084347
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: Akt2
• Ensembl ID: ENSMUSG00000004056
• Aliases: Akt2, 2410016A19Rik, AW554154, PKB, PKBbeta
• Description: thymoma viral proto-oncogene 2 [Source:MGI Symbol;Acc:MGI:104874]

Sequence

>retro_mmus_694
ATGAATGAGGTATCTGTCATCAAAGAAGGCTGGCTCCACAAACGTGGTGAATACATCAAGACCTGGAGGCCACGGTACTT
CCTTCTGAAGAGTGATGGATCTTTCATTGGGTATAAGGAGAGGCCCGAGTCCCCTGACCAGACCTTACCCCCCCCCCCCA
ACAATTTCTCTGTAGCAGAATGCCAGCTGATGAAGACTGAGAGGCCACGACCCAACACCTTTGTCATACGCTGCCTGCAG
TGGACCACAGTCATCGAGAGGACCTTCCATGTAGACTCTCCAGATGAGAGGGAAGAGTGAATGCGGGCTATCCAGATGGT
CGCCAACAGTCTGAAGCAGCGGGGCCCAGGTGAGGACGCCATGGATTACAAGTGTGGCTCCCCCAGTGACTCTTCCACAT
CTGAGATGATGGAGGTAGCTGTCAACAAGGCACGGGCCAAAGTGACCATGAATGACTTCGATTATCTCAAACTCCTCGGC
AAGGGCACCTTCGGCAAGGTCATTTTGGTTCGAGAGAAGGCCACTGGCCGCTATTATGCCATGAAGATCCTGCGCAAGGA
GGTCATCATTGCGAAGGATGAAGTCGCCCACACAGTCACAGAGAGCCGGGTTCTGCAGAATACCAGGCACCCCTTCCTTA
CAGCCCTCAAGTATGCCTTCCAGACCCATGACCGCCTGTGCTTTGTGATGGAGTATGCCAACGGGGGTGAGCTGTTTTTC
CACCTCTCTCGGGAGCGAGTCTTCACGGAGGATCGGGCGCGCTTTTACGGAGCAGAGATTGTGTCAGCTCTGGAGTATTT
GCACTCGAGAGATGTGGTGTACCGTGACATCAAGCTGGAAAACCTTATGTTGGACAAAGATGGCCACATCAAGATCACTG
ACTTTGGCTTGTGCAAAGAGGGCATCAGTGATGGAGCCACCATGAAAACCTTCTGTGGTACCCCGGAGTACTTGGCGCCT
GAGGTGCTAGAGGACAATGACTATGGGCGCGCAGTGGACTGGTGGGGGCTGTGTGTGGTCATGTATGAGATGATGTGTGG
CCGCCTGCCATTCTACAACCAGGACCACGAGCGCCTCTTTGAGCTCATCCTTATGGAGGAGATCCGCTTCCCGCGCACAC
TCGGGCCAGAGGCCAAGTCCCTGCTGGCTGGGCTGCTGAAGAAGGACCCAAAGCAGAGGCTCGGCGGAGGTCCCAGTGAT
GCGAAGGAGGTGATGGAGCATAGGTTCTTCCTCAGCATCAACTGGCAGGACGTGGTACAGAAAAAGCTCCTGCCACCCTT
CAAACCTCAGGTCACTTCAGAAGTGAACACAAGGTACTTTGATGAGGAGTTCACCGCCCAGTCCATCACAATCACACCCC
CAGACCGATATGACAGCCTGGATCCGCTGGAACTGGACCAGCGGACGCACTTCCCCCAGTTCTCCTACTCAGCCAGCATC
CGAGAG

Alignment

ORF - retro_mmus_694 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 98.13 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 1
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPEAPDQTLPPL-NNFSVAECQLMKTERPR
          MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPE.PDQTL....NNFSVAECQLMKTERPR
Retrocopy MNEVSVIKEGWLHKRGEYIKTWRPRYFLLKSDGSFIGYKERPESPDQTLXXXXNNFSVAECQLMKTERPR

Parental  PNTFVIRCLQWTTVIERTFHVDSPDEREEWMRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVA
          PNTFVIRCLQWTTVIERTFHVDSPDEREE.MRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVA
Retrocopy PNTFVIRCLQWTTVIERTFHVDSPDEREE*MRAIQMVANSLKQRGPGEDAMDYKCGSPSDSSTSEMMEVA

Parental  VNKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRH
          VNKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRH
Retrocopy VNKARAKVTMNDFDYLKLLGKGTFGKVILVREKATGRYYAMKILRKEVIIAKDEVAHTVTESRVLQNTRH

Parental  PFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLE
          PFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLE
Retrocopy PFLTALKYAFQTHDRLCFVMEYANGGELFFHLSRERVFTEDRARFYGAEIVSALEYLHSRDVVYRDIKLE

Parental  NLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLGVVMYEMMCGRLP
          NLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGL.VVMYEMMCGRLP
Retrocopy NLMLDKDGHIKITDFGLCKEGISDGATMKTFCGTPEYLAPEVLEDNDYGRAVDWWGLCVVMYEMMCGRLP

Parental  FYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQ
          FYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQ
Retrocopy FYNQDHERLFELILMEEIRFPRTLGPEAKSLLAGLLKKDPKQRLGGGPSDAKEVMEHRFFLSINWQDVVQ

Parental  KKLLPPFKPQVTSEVDTRYFDDEFTAQSITITPPDRYDSLDPLELDQRTHFPQFSYSASIRE
          KKLLPPFKPQVTSEV.TRYFD.EFTAQSITITPPDRYDSLDPLELDQRTHFPQFSYSASIRE
Retrocopy KKLLPPFKPQVTSEVNTRYFDEEFTAQSITITPPDRYDSLDPLELDQRTHFPQFSYSASIRE

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.16 RPM	46.77 RPM
SRP007412_cerebellum	0.35 RPM	59.66 RPM
SRP007412_heart	0.59 RPM	123.77 RPM
SRP007412_kidney	0.94 RPM	67.12 RPM
SRP007412_liver	0.41 RPM	69.25 RPM
SRP007412_testis	0.73 RPM	50.82 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_694 was not detected

EST

No EST(s) were mapped for retro_mmus_694 retrocopy.

TSS

No TSS is located nearby retro_mmus_694 retrocopy 5' end.

Lab

retro_mmus_694 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_694 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 3 parental genes, and 4 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000014574	1 retrocopy	retro_cjac_1138,
Mus musculus	ENSMUSG00000004056	1 retrocopy	retro_mmus_694 ,
Sus scrofa	ENSSSCG00000002989	2 retrocopies	retro_sscr_1164, retro_sscr_1200,