RetrogeneDB ID: retro_cfam_1866

Retrocopy location

• Organism: Dog (Canis familiaris)
• Coordinates: 7:77500519..77501101(+)
• Located in intron of: ENSCAFG00000018787

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: CHMP1A
• Ensembl ID: ENSCAFG00000019822
• Aliases: None
• Description: charged multivesicular body protein 1A [Source:HGNC Symbol;Acc:8740]

Sequence

>retro_cfam_1866
ATGGAGAAACACCTGTTCAACCTAAAGTTCGCCGCCAAAGAACTCAGCAGGAGTGCCAAAAAATGCGACAAGGAAGAAAA
GGCCGAAAAGGCCAAGATTAAAAAGGCCATTCAGAAGGGCAACATGGAAGTGGCGAGGATACACGCCGAAAACGCCATTC
GCCAGAAGAACCAGGCGGTGAATTTCCTGAGGATGAGTGCGCGGGTCGATGCGGTGGCTGCCAGAGTGCAGACGGCGGTG
ACGATGGGCAAGGTGACCAAGTCGATGGCCGGTGTGGTTAAGTCGATGGACGCAACGTTGAAGACCATGAATCTGGAGAA
GATCTCTGCTTTGATGGACAAATTTGAGCACCAGTTTGAAACGCTGGACGTCCAGACGCAGCAGATGGAAGACACGATGA
GCAGCACGACTACGCTGACCACCCCCCAGAACCAAGTGGATATGCTGCTCCAGGAAATGGCAGACGAGGCTGGCCTAGAC
CTCAACATGGAGCTGCCGCAGGGCCAGACCGGCTCCGTGGGCACCAGCGTGGCCTCCGCCGAGCAGGATGAACTGTCCCA
GAGACTGGCCCGCCTGCGGGAT

Alignment

ORF - retro_cfam_1866 Open Reading Frame is conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 56.63 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  MDDTLFQLKFTAKQLEKLAKKAEKDSKAEQAKVKKALQQKNVECARVYAENAIRKKNEGVNWLRMASRVD
          M...LF.LKF.AK.L...AKK..K..KAE.AK.KKA.Q..N.E.AR..AENAIR.KN..VN.LRM..RVD
Retrocopy MEKHLFNLKFAAKELSRSAKKCDKEEKAEKAKIKKAIQKGNMEVARIHAENAIRQKNQAVNFLRMSARVD

Parental  AVASKVQTAVTMKGVTKNMAQVTKALDRALSTMDLQKVSAVMDRFEQQVQNLDVHTAVMEDSMSSATTLT
          AVA..VQTAVTM..VTK.MA.V.K..D..L.TM.L.K.SA.MD.FE.Q...LDV.T..MED.MSS.TTLT
Retrocopy AVAARVQTAVTMGKVTKSMAGVVKSMDATLKTMNLEKISALMDKFEHQFETLDVQTQQMEDTMSSTTTLT

Parental  TPQEQVDSLIMQIAEENGLEVLDQLNQLPEGASAVGESSVRSQEDQLSRRLAALRN
          TPQ.QVD.L....A.E.GL.....L.Q...G...VG.S......D.LS.RLA.LR.
Retrocopy TPQNQVDMLLQEMADEAGLDLNMELPQGQTG--SVGTSVASAEQDELSQRLARLRD

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP012049_cerebellum	26.84 RPM	30.74 RPM
SRP017611_brain	23.66 RPM	19.37 RPM
SRP017611_kidney	26.61 RPM	24.37 RPM
SRP017611_liver	7.03 RPM	11.31 RPM

ChIP-Seq

Canis familiaris was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_cfam_1866 retrocopy.

TSS

Canis familiaris was not studied using FANTOM5 data.

Lab

retro_cfam_1866 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_cfam_1866 has 8 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_75
Pan troglodytes	retro_ptro_1278
Gorilla gorilla	retro_ggor_1368
Pongo abelii	retro_pabe_1581
Callithrix jacchus	retro_cjac_1147
Oryctolagus cuniculus	retro_ocun_1508
Rattus norvegicus	retro_rnor_1361
Equus caballus	retro_ecab_991

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Parental genes homology:

Parental genes homology involve 13 parental genes, and 17 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000001216	1 retrocopy	retro_amel_1749,
Bos taurus	ENSBTAG00000033331	1 retrocopy	retro_btau_1048,
Canis familiaris	ENSCAFG00000019822	1 retrocopy	retro_cfam_1866 ,
Callithrix jacchus	ENSCJAG00000010826	1 retrocopy	retro_cjac_1147,
Homo sapiens	ENSG00000131165	2 retrocopies	retro_hsap_359, retro_hsap_75,
Macaca mulatta	ENSMMUG00000014909	3 retrocopies	retro_mmul_1325, retro_mmul_476, retro_mmul_706,
Mustela putorius furo	ENSMPUG00000007281	1 retrocopy	retro_mputfur_383,
Mus musculus	ENSMUSG00000000743	1 retrocopy	retro_mmus_2260,
Oryctolagus cuniculus	ENSOCUG00000023371	1 retrocopy	retro_ocun_1508,
Otolemur garnettii	ENSOGAG00000004883	1 retrocopy	retro_ogar_170,
Pongo abelii	ENSPPYG00000007667	1 retrocopy	retro_pabe_344,
Pan troglodytes	ENSPTRG00000008483	2 retrocopies	retro_ptro_1278, retro_ptro_283,
Ictidomys tridecemlineatus	ENSSTOG00000023813	1 retrocopy	retro_itri_1311,