RetrogeneDB ID: retro_mmus_3358

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 9:78767109..78767480(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Myl6
• Ensembl ID: ENSMUSG00000090841
• Aliases: Myl6, ESMLC, LC17, LC17-GI, LC17-NM, LC17A, LC17B, MLC-3, MLC1SM, MLC3SM, MLC3nm, Myln
• Description: myosin, light polypeptide 6, alkali, smooth muscle and non-muscle [Source:MGI Symbol;Acc:MGI:109318]

Sequence

>retro_mmus_3358
CAGGTGATGCTAAAACACTGTACAGCCAATTTGGTAAGGGCTCTGGGTCAGAACACCAAGGTGCTCAAGTTCCTGGGGAA
CCCCAAGGGTGATGAGATGGATATGAAGGTGCTGGACTTTGAGCACTTCCTGTCCATGCTGCACACTGGGACCACAAACA
AGGACCAGGGATCTTATGAGGACCAGGTTAAAGGCCGTCATGTACTTGACAAGGACGGAAATGGTACAATCATGGGTGGT
GAAATCTGTCATGTCCCTGTTTGGATGAGATGACAGAGGACGAAGTAGAGATGCTAATGACAGGTCATGAGGACAGAAAT
GGTTGTATCAGCTACAAAGAGCTTGTCTGTGTCTGGATGGTGCTGAATGGG

Alignment

ORF - retro_mmus_3358 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 57.69 %
• Parental protein coverage: 83.44 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  KILYSQC-GDVMRALGQNPTNAEVLKVLGNPKSDEMNVKVLDFEHFLPMLQTVAKNKDQGTYEDYVEGLR
          ......C.....RALGQ...N..VLK.LGNPK.DEM..KVLDFEHFL.ML.T...NKDQG.YED.V.G..
Retrocopy QVMLKHCTANLVRALGQ---NTKVLKFLGNPKGDEMDMKVLDFEHFLSMLHTGTTNKDQGSYEDQVKGRH

Parental  VFDKEGNGTVMGAEIRHVLVTLGEKMT-EEEVEMLVAGHEDSNGCINYEEL--VRMVLNG
          V.DK.GNGT.MG.EI.HV.V......T.E.E..M...GHED.NGCI.Y.EL..V.MVLNG
Retrocopy VLDKDGNGTIMGGEICHVPVWMR*QRT<EVEMLM--TGHEDRNGCISYKELVCVWMVLNG

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	86.35 RPM
SRP007412_cerebellum	0.00 RPM	88.75 RPM
SRP007412_heart	0.00 RPM	73.25 RPM
SRP007412_kidney	0.00 RPM	113.42 RPM
SRP007412_liver	0.00 RPM	60.32 RPM
SRP007412_testis	0.00 RPM	23.83 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_3358 was not detected

EST

No EST(s) were mapped for retro_mmus_3358 retrocopy.

TSS

No TSS is located nearby retro_mmus_3358 retrocopy 5' end.

Lab

retro_mmus_3358 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_3358 has 0 orthologous retrocopies within eutheria group .

---

Parental genes homology:

Parental genes homology involve 11 parental genes, and 30 retrocopies.

Species	Parental gene accession	Retrocopies number
Macropus eugenii	ENSMEUG00000001272	1 retrocopy	retro_meug_2172,
Macaca mulatta	ENSMMUG00000031838	6 retrocopies	retro_mmul_1633, retro_mmul_1663, retro_mmul_211, retro_mmul_2221, retro_mmul_2410, retro_mmul_96,
Mus musculus	ENSMUSG00000061816	1 retrocopy	retro_mmus_973,
Mus musculus	ENSMUSG00000090841	7 retrocopies	retro_mmus_1281, retro_mmus_1504, retro_mmus_2328, retro_mmus_3231, retro_mmus_3333, retro_mmus_3358 , retro_mmus_434,
Nomascus leucogenys	ENSNLEG00000000205	2 retrocopies	retro_nleu_2736, retro_nleu_779,
Oryctolagus cuniculus	ENSOCUG00000017371	1 retrocopy	retro_ocun_1300,
Otolemur garnettii	ENSOGAG00000034596	1 retrocopy	retro_ogar_2549,
Pongo abelii	ENSPPYG00000004647	3 retrocopies	retro_pabe_1467, retro_pabe_1864, retro_pabe_538,
Sarcophilus harrisii	ENSSHAG00000004472	6 retrocopies	retro_shar_137, retro_shar_318, retro_shar_452, retro_shar_510, retro_shar_719, retro_shar_765,
Sus scrofa	ENSSSCG00000025467	1 retrocopy	retro_sscr_829,
Ictidomys tridecemlineatus	ENSSTOG00000023318	1 retrocopy	retro_itri_1478,