RetrogeneDB ID: retro_mdom_1582

Retrocopy location

• Organism: Opossum (Monodelphis domestica)
• Coordinates: 6:47725834..47726346(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: NDUFB9
• Ensembl ID: ENSMODG00000001831
• Aliases: None
• Description: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa [Source:HGNC Symbol;Acc:7704]

Sequence

>retro_mdom_1582
CCAGGTGCCTATCTGACCCATTAGCATAAGATGATGCAGCTCTACAGCCAGGTGTTCACCACCTCAAGACCTGGATATTT
AGAGGGAATGGATATTGGTTCAGAGCTTGATTGCTGAGAGCTCAGTTTGAGAGTTATTAGAATGAAAAAGATGAAGGCTA
CAAACTGCTTTGGGAGGCTGAGCAAGAATTTGGGGCTTGCCAACATCCTCAGCCATATATCTTCCCTGATTCCCCTGGAG
GCATATCCTTTGAAATATATGAATATTACAAGATTCCTGAGTGGTGCTTAGAATACTGGCTCCCCTCTAAGAAGGCAATG
TACCCAGATTACTTTGCTAAGAAGGAGAAGTGGAAGAAACTACACTTGGAAAGTTGATTTTGTAATGTTCAGCAATTGAA
GGAGAAAACTCCACCTGATGGTTCTAAGACTGAAGCTCTGTCTCCAGTCTGAAAGAAGGGTCATTTGCCACCATTATGGT
GGCATATTGTCACCAGGCCCTGAGAGCACCCC

Alignment

ORF - retro_mdom_1582 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 68.39 %
• Parental protein coverage: 97.19 %
• Number of stop codons detected: 6
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  PGAYLTHRQKVLRLYKRA-LRHLESWIVRRDEYRFRACLLRDRFERNRNENDMMKATKLLWEGEKEFWAN
          PGAYLTH..K...LY......HL..WI.R...Y.FRA.LLR..FE...NE.D.....KLLWE.E.EF.A.
Retrocopy PGAYLTH*HKMMQLYSQV<VHHLKTWIFRGNGYWFRA*LLRAQFESY*NEKD--EGYKLLWEAEQEFGAC

Parental  QHPQPYIFPDSPGGTSFERYECYKVPEWCLDYWHPSEKAMYPDYFAKREKWKKLRLESWDREVQQLKEET
          QHPQPYIFPDSPGG.SFE.YE.YK.PEWCL.YW.PS.KAMYPDYFAK.EKWKKL.LES....VQQLKE.T
Retrocopy QHPQPYIFPDSPGGISFEIYEYYKIPEWCLEYWLPSKKAMYPDYFAKKEKWKKLHLES*FCNVQQLKEKT

Parental  PPDGPKTEALPPARKEGHLPPLWWHIVTRPRERP
          PPDG.KTEAL.P..K.GHLPPLWWHIVTRP.E.P
Retrocopy PPDGSKTEALSPV*KKGHLPPLWWHIVTRP*EHP

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	0.00 RPM
SRP007412_cerebellum	0.00 RPM	0.00 RPM
SRP007412_heart	0.00 RPM	0.00 RPM
SRP007412_kidney	0.00 RPM	0.00 RPM
SRP007412_liver	0.00 RPM	0.00 RPM
SRP007412_testis	0.00 RPM	0.00 RPM

ChIP-Seq

Monodelphis domestica was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mdom_1582 retrocopy.

TSS

Monodelphis domestica was not studied using FANTOM5 data.

Lab

retro_mdom_1582 was not experimentally validated.

Homology

Retrocopy orthology:

Monodelphis domestica does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 18 parental genes, and 30 retrocopies.

Species	Parental gene accession	Retrocopies number
Anolis carolinensis	ENSACAG00000010022	1 retrocopy	retro_acar_132,
Ailuropoda melanoleuca	ENSAMEG00000015852	2 retrocopies	retro_amel_1680, retro_amel_553,
Callithrix jacchus	ENSCJAG00000012818	1 retrocopy	retro_cjac_2125,
Equus caballus	ENSECAG00000018238	1 retrocopy	retro_ecab_541,
Felis catus	ENSFCAG00000004678	1 retrocopy	retro_fcat_689,
Homo sapiens	ENSG00000147684	2 retrocopies	retro_hsap_3905, retro_hsap_4112,
Gorilla gorilla	ENSGGOG00000015883	2 retrocopies	retro_ggor_2632, retro_ggor_2759,
Microcebus murinus	ENSMICG00000017481	1 retrocopy	retro_mmur_1494,
Myotis lucifugus	ENSMLUG00000003765	1 retrocopy	retro_mluc_1339,
Macaca mulatta	ENSMMUG00000015493	1 retrocopy	retro_mmul_1736,
Monodelphis domestica	ENSMODG00000001831	3 retrocopies	retro_mdom_1176, retro_mdom_1193, retro_mdom_1582 ,
Nomascus leucogenys	ENSNLEG00000005484	2 retrocopies	retro_nleu_1141, retro_nleu_578,
Otolemur garnettii	ENSOGAG00000030772	2 retrocopies	retro_ogar_1654, retro_ogar_925,
Pongo abelii	ENSPPYG00000018869	3 retrocopies	retro_pabe_2643, retro_pabe_3182, retro_pabe_3366,
Pan troglodytes	ENSPTRG00000020569	2 retrocopies	retro_ptro_2656, retro_ptro_2791,
Pteropus vampyrus	ENSPVAG00000009153	1 retrocopy	retro_pvam_243,
Ictidomys tridecemlineatus	ENSSTOG00000021955	1 retrocopy	retro_itri_611,
Tupaia belangeri	ENSTBEG00000006895	3 retrocopies	retro_tbel_1901, retro_tbel_2663, retro_tbel_610,