RetrogeneDB ID: retro_oana_116

Retrocopy location

• Organism: Platypus (Ornithorhynchus anatinus)
• Coordinates: Ultra437:788840..789371(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: DFFB
• Ensembl ID: ENSOANG00000013652
• Aliases: None
• Description: DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase) [Source:HGNC Symbol;Acc:2773]

Sequence

>retro_oana_116
GGTAAGAATAAATACAGTTGTGAGACCAGAATCCAGAGGTATATGAAAGAGGTTAACACTTCTGCTTCATCTGGGGGTCC
CAAGGCTCAGGCAGAATATCTGACCGTCATTGGCCATATGGGAGAGAAGCTGAAAATGGCCCAGCCTAACGGGGAATACT
TGGACTGGCAAGAAAACAGGGCCCAGGTCTATGCAACAGAGGGCAGTTTCTGTTGCCAAGGTTCACTGGACACTGACAGC
TACTCAACTGAACATTCCATCAACTCTTACACCAACAGGTAGAGCAGAATTCTTTTCAGCATGTGGAATCTGGATCATAG
AATAGAAAAGCAATGCACTGTCATGATGACCCCAACAGAAGCAGTCAGGGAACAAATTAGAATAGAAATAGATTGGGAGT
ACTTCTATAACATCCTCTTTACTTCAGAAGATCTAAATTTAGTGCCTATAGCCTGATACAAGAAAACCAGCCCTAAACTC
AGCTGCAATAAAACTAAGATTTATAGGGCCGGAGTGGCCGTTTCCCGAAAA

Alignment

ORF - retro_oana_116 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 65.54 %
• Parental protein coverage: 90.77 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 0

Retrocopy - Parental Gene Alignment:

Parental  GYMRYSCESRIRSYMKEVNTSASAIGPKAQAEYLKIIGCMGEKLKAAQYNGGYFDRREKRARLCTPEGWF
          G...YSCE.RI..YMKEVNTSAS..GPKAQAEYL..IG.MGEKLK.AQ.NG.Y.D..E.RA.....EG.F
Retrocopy GKNKYSCETRIQRYMKEVNTSASSGGPKAQAEYLTVIGHMGEKLKMAQPNGEYLDWQENRAQVYATEGSF

Parental  SCQGPFDIDSCLSKHSINPYSNRESRILFSTWNLDHIIEKKRTVVPTLAEAVREQDGREVDWEYFYNLLF
          .CQG..D.DS....HSIN.Y.NR.SRILFS.WNLDH.IEK..TV..T..EAVREQ...E.DWEYFYN.LF
Retrocopy CCQGSLDTDSYSTEHSINSYTNR*SRILFSMWNLDHRIEKQCTVMMTPTEAVREQIRIEIDWEYFYNILF

Parental  TSENLKLVHIACHKKTSHNLSCDKTRIYRARAAVPQK
          TSE.L.LV.IA..KKTS..LSC.KT.IYRA..AV..K
Retrocopy TSEDLNLVPIA*YKKTSPKLSCNKTKIYRAGVAVSRK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	1.06 RPM
SRP007412_cerebellum	0.00 RPM	1.26 RPM
SRP007412_heart	0.00 RPM	0.62 RPM
SRP007412_kidney	0.00 RPM	0.89 RPM
SRP007412_liver	0.00 RPM	2.17 RPM
SRP007412_testis	0.00 RPM	2.37 RPM

ChIP-Seq

Ornithorhynchus anatinus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_oana_116 retrocopy.

TSS

Ornithorhynchus anatinus was not studied using FANTOM5 data.

Lab

retro_oana_116 was not experimentally validated.

Homology

Retrocopy orthology:

Ornithorhynchus anatinus does not belong to any of the species groups (eutheria, teleost or neognath), studied for retrocopy-based homology. For more information about studied groups, please go to help section.

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 9 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000002834	1 retrocopy	retro_cjac_616,
Homo sapiens	ENSG00000169598	1 retrocopy	retro_hsap_4240,
Gorilla gorilla	ENSGGOG00000013452	1 retrocopy	retro_ggor_2841,
Macaca mulatta	ENSMMUG00000015174	1 retrocopy	retro_mmul_1150,
Ornithorhynchus anatinus	ENSOANG00000013652	5 retrocopies	retro_oana_116 , retro_oana_117, retro_oana_125, retro_oana_27, retro_oana_91,