RetrogeneDB ID: retro_ecab_582

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 21:39710575..39711448(-)
• Located in intron of: ENSECAG00000014223

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: None
• Ensembl ID: ENSECAG00000010030
• Aliases: None
• Description: None

Sequence

>retro_ecab_582
AGACAAAGGAATACTATCCAAGCAAGGGAACAGGACACAACCCCAGAAAAAGGACTAAATGAAACAGAAACAGGTGACAT
ACTCAACAAAGAGTTCAAACAAAATATCATAAGGATGCTCACAGATATTGGGAGAAGACTGGGATGAACACAGTGAGCTT
ATCAGCAAAGAACCAGAAGATATAAAAAAGAACCAACCAGAAATGAAGAATACAATACTGGAAATGAAAAATTCACTAGA
GGGATTCAATAGTGGAGTAGAGGATGCAGAAGAATGGATCAGCAAGCTAGATGAAAGACTAGAGGAAATCACCCAAGCTG
AACAGAAAAAGGAATTAGACAGAATGAGAAGAGTATAAGGGAACTCTGGGACAATATCAAGTGTGCTAATTTTCAGATTA
TAGGTGTCCCAGAAGGAGAAGAGAGAGACAAAGGGGCAGAAAATTTATTTGAAGAAATAATAGATGAAAAGTTTCCTAAC
CTAAGAACAGACATCCAAGTACAGGAAGCACAGAGAGCTCCAAACAAGATAAGCCCAAAGAGGCCCACACCAAGACATAT
TATAATTAAAATGTCCAAAATTAAAGATAAAGAGAGAATCCTAAAAGCAGCAAGAGAAAGGCCACAAGTGACATACAAAG
GGAAGCCCATAAGGCTATCAGCGGACATCTCAGCTGAGACCCTACAGGTAAGAAGAGAATGACACAACGTATTTAAAGTG
CTAAAAGGAAAATGTCTACAGCCAAGAATACTCTATCCATGAAGGCTGTCATTCAGAATGGAAGGAGAGAGAAAGTGCTT
CCCAGACAAGCAAAAATTAAAGGAGTTTATCACCAAGAAACCAGTTCTGGAAGAAACGCTGAAGGGACTTATT

Alignment

ORF - retro_ecab_582 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 66.55 %
• Parental protein coverage: 99.31 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  RQRSTFQVKEQDKTPEKELNETEISNLPDKVFKQKV-KLLTDLGRRM-DEHSENFNKELENIKK-QSEMK
          RQR.T.Q..EQD.TPEK.LNETE......K.FKQ.....LTD.GRR..DEHSE...KE.E.IKK.Q.EMK
Retrocopy RQRNTIQAREQDTTPEKGLNETETGDILNKEFKQNIIRMLTDIGRRL>DEHSELISKEPEDIKKNQPEMK

Parental  NTIMEMKNSLEGLNSRVDDTEERISKLDERLEEVTQAEQIKEK-MKKTDSLRDLWDNIKCTKIRITGVPE
          NTI.EMKNSLEG.NS.V.D.EE.ISKLDERLEE.TQAEQ.KE.......S.R.LWDNIKC....I.GVPE
Retrocopy NTILEMKNSLEGFNSGVEDAEEWISKLDERLEEITQAEQKKEL<RQNEKSIRELWDNIKCANFQIIGVPE

Parental  -EEGDKGAE-IYLIKIAENFPKLQKEADFQVQEAQNAPNRINPKRHTPRHIINKISNPGRNKERILKAAR
          .EE.DKGAE......I.E.FP.L....D.QVQEAQ.APN.I.PKR.TPRHII.K.S.....KERILKAAR
Retrocopy GEERDKGAENLFEEIIDEKFPNLR--TDIQVQEAQRAPNKISPKRPTPRHIIIKMSKI-KDKERILKAAR

Parental  ERQQVTYKGNSIRLSADLSAETLQARRKWHSIFKVLNRK-KPPRILYLARSSFIMEGEIKCFPDKQKLKE
          ER.QVTYKG..IRLSAD.SAETLQ.RR..H..FKVL..K...PRILY..R.SF.MEGE.KCFPDKQKLKE
Retrocopy ERPQVTYKGKPIRLSADISAETLQVRRE*HNVFKVLKGKCLQPRILYP*RLSFRMEGERKCFPDKQKLKE

Parental  FITKKPALQEMLKGLI
          FITKKP.L.E.LKGLI
Retrocopy FITKKPVLEETLKGLI

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.00 RPM	0.00 RPM
SRP021940_cerebellum	0.00 RPM	0.00 RPM
SRP021940_embryo	0.00 RPM	0.03 RPM
SRP021940_placental_villous	0.00 RPM	0.00 RPM
SRP021940_synovial_membrane	0.00 RPM	0.00 RPM
SRP021940_testis	0.00 RPM	0.00 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_582 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_582 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_582 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 54 retrocopies.

Species	Parental gene accession	Retrocopies number
Equus caballus	ENSECAG00000010030	21 retrocopies	retro_ecab_1021, retro_ecab_1023, retro_ecab_1035, retro_ecab_1071, retro_ecab_1089, retro_ecab_136, retro_ecab_182, retro_ecab_201, retro_ecab_281, retro_ecab_306, retro_ecab_486, retro_ecab_521, retro_ecab_537, retro_ecab_582 , retro_ecab_590, retro_ecab_658, retro_ecab_670, retro_ecab_722, retro_ecab_786, retro_ecab_974, retro_ecab_996,
Equus caballus	ENSECAG00000016527	5 retrocopies	retro_ecab_214, retro_ecab_642, retro_ecab_879, retro_ecab_888, retro_ecab_993,
Equus caballus	ENSECAG00000020332	9 retrocopies	retro_ecab_1018, retro_ecab_117, retro_ecab_125, retro_ecab_346, retro_ecab_54, retro_ecab_604, retro_ecab_774, retro_ecab_775, retro_ecab_878,
Equus caballus	ENSECAG00000022034	4 retrocopies	retro_ecab_1039, retro_ecab_278, retro_ecab_821, retro_ecab_858,
Equus caballus	ENSECAG00000023806	15 retrocopies	retro_ecab_1040, retro_ecab_1050, retro_ecab_1073, retro_ecab_1077, retro_ecab_1108, retro_ecab_176, retro_ecab_241, retro_ecab_298, retro_ecab_339, retro_ecab_714, retro_ecab_778, retro_ecab_781, retro_ecab_852, retro_ecab_855, retro_ecab_953,