RetrogeneDB ID: retro_ecab_778

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 4:25900574..25901373(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: None
• Ensembl ID: ENSECAG00000023806
• Aliases: None
• Description: None

Sequence

>retro_ecab_778
ATGAGGAGATAAAGGAATACATTCCAAGTAAGGAAACAGGATAAAACCACAGAAAAAGAATTAAATGAAAGAGAAATAAA
TTATCTACCTGACAAAGAGTTCAACCAAAGAGTCATAAGGATGCTCATTGATCTTGGGAGAAGAACTCAGTGAGAATATC
AACAAAGAATTGGAAAATATAAAAAAGAACCAATCTGAAATGAAGAATACAATACAAGAAATGAAAACCTTCACTAGAGG
GACTCAATAGCAGAGTAGACACAGAAGAATGAATCAGCAAGCTGGATGAAAGACTAGAGGAAATCACACAAGCTGAACAG
TTAAAAGAAAAAAATTGAAAAAGAGTGAGGGCAGTCTAAGGGACCTCTGGGACAACATCTATCATACTAATATCTGTGTT
ATAGTTGTCCCAGGAGGAGAAGAGAGAGACAAAGGGGCAGAGAATCTATTTGAAGAAATCATAGCTGAAAACTTTCCTAA
CCTAAGGAAGGAAACAGACATCCAAGTACAGGAAGCACAGAGAGCACCAAATAAGAGAAACCCAAGGAGGCCCACACCAA
GACACATTATAATTGAAAGGTCAAGAATTAAAGATGAGAGAGAATCCTAAAAGCCACAAGAGAAGGGCAACAAGTTACAT
ACAGAGGAAACCCCATAATGCTATCTGCTGACTTCTCAGCAGAAACTTTACAGGCTAAAAGGGAGTGGCATGATATATTT
AAAGTGCTGAAAGGAAAAAAACCTACAGCCAAGAATACTCTACCCAGCAAGCTTATCACTCAGAATGGAAGGAGAGATA

Alignment

ORF - retro_ecab_778 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 70.18 %
• Parental protein coverage: 96.09 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 5

Retrocopy - Parental Gene Alignment:

Parental  MRRQRNMFHARVQDKTPEKELNETQRDNLPDKEFKQKGIRMLID-LGRRMDKHSENVNKELENIKKSQSE
          MRR.RN.F..R.QDKT.EKELNE.....LPDKEF.Q..IRMLID.LG......SEN.NKELENIKK.QSE
Retrocopy MRR*RNTFQVRKQDKTTEKELNEREINYLPDKEFNQRVIRMLID>LGEEL---SENINKELENIKKNQSE

Parental  MKSTILKMKN-SLEGLNSRVDDTEEWIKKLDEKLEEISQVNQ-VKEKTILKNDSKIKELWDNIKHANIRI
          MK.TI..MK..SLEGLNSRVD.TEE.I.KLDE.LEEI.Q..Q.V..............LWDNI.H.NI..
Retrocopy MKNTIQEMKT>SLEGLNSRVD-TEE*ISKLDERLEEITQAEQ<VXXXXXXXSEGSLRDLWDNIYHTNICV

Parental  ISVPGGEERHKGEENLFEEIIDENFPNLRKETDIQVQEEQRAPNKSSPKRPTPIHIIIKMSKIKDK-ERI
          I.VPGGEER.KG.ENLFEEII.ENFPNLRKETDIQVQE.QRAPNK..P.RPTP.HIII..S.IKD..ERI
Retrocopy IVVPGGEERDKGAENLFEEIIAENFPNLRKETDIQVQEAQRAPNKRNPRRPTPRHIIIERSRIKDE<ERI

Parental  LKAARERPEVTYKGKPIRLSADFSAETLQATREWHDIFKVLKG-KNLQPRIFYPSRSSFRMEGEI
          LKA.RE...VTY.G.PI.LSADFSAETLQA.REWHDIFKVLKG.KNLQPRI.YP...S.RMEGEI
Retrocopy LKATREGQQVTYRGNPIMLSADFSAETLQAKREWHDIFKVLKG>KNLQPRILYPASLSLRMEGEI

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.00 RPM	0.12 RPM
SRP021940_cerebellum	0.00 RPM	0.00 RPM
SRP021940_embryo	0.00 RPM	0.28 RPM
SRP021940_placental_villous	0.00 RPM	0.00 RPM
SRP021940_synovial_membrane	0.00 RPM	0.08 RPM
SRP021940_testis	0.00 RPM	0.00 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_778 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_778 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_778 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 54 retrocopies.

Species	Parental gene accession	Retrocopies number
Equus caballus	ENSECAG00000010030	21 retrocopies	retro_ecab_1021, retro_ecab_1023, retro_ecab_1035, retro_ecab_1071, retro_ecab_1089, retro_ecab_136, retro_ecab_182, retro_ecab_201, retro_ecab_281, retro_ecab_306, retro_ecab_486, retro_ecab_521, retro_ecab_537, retro_ecab_582, retro_ecab_590, retro_ecab_658, retro_ecab_670, retro_ecab_722, retro_ecab_786, retro_ecab_974, retro_ecab_996,
Equus caballus	ENSECAG00000016527	5 retrocopies	retro_ecab_214, retro_ecab_642, retro_ecab_879, retro_ecab_888, retro_ecab_993,
Equus caballus	ENSECAG00000020332	9 retrocopies	retro_ecab_1018, retro_ecab_117, retro_ecab_125, retro_ecab_346, retro_ecab_54, retro_ecab_604, retro_ecab_774, retro_ecab_775, retro_ecab_878,
Equus caballus	ENSECAG00000022034	4 retrocopies	retro_ecab_1039, retro_ecab_278, retro_ecab_821, retro_ecab_858,
Equus caballus	ENSECAG00000023806	15 retrocopies	retro_ecab_1040, retro_ecab_1050, retro_ecab_1073, retro_ecab_1077, retro_ecab_1108, retro_ecab_176, retro_ecab_241, retro_ecab_298, retro_ecab_339, retro_ecab_714, retro_ecab_778 , retro_ecab_781, retro_ecab_852, retro_ecab_855, retro_ecab_953,