RetrogeneDB ID: retro_ecab_858

Retrocopy location

• Organism: Horse (Equus caballus)
• Coordinates: 5:46437211..46437926(-)
• Located in intron of: ENSECAG00000016587

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: None
• Ensembl ID: ENSECAG00000022034
• Aliases: None
• Description: None

Sequence

>retro_ecab_858
GAAAACCTAGAAAGAAGTCAAAAAGAAATTCTTGAGCTAAAAAGTATAATAACTGAAATGAAAAATTCACTAGAGGGATT
CAAAGGCAGATTTGAGCAGGCAAAAGAAAGTGTTAACAAATTTGAAGATAGGGCAATTAAAATTATTGAGTCTGAAGGAC
AGAAAGAAAAAAATTGAAGAATAATGAACAGAGCCCAGGCAACCATGAGACATCAACAAAAGTGAACCAACATACACATT
CTGGGACTCCCAGAAGGAGAAAACAGAGAAAAAGGGACAGAGAGATTATCTGAAGAATTAATGGCCAAAAACTTCCCAAG
TTTGATGAAAGACACGAATATAAACATACTAGAAGCTCAAGGAATTCCAAATTGGATGAACTCAAAGAGGTGCACACTGA
GTCACATTAACATCAAACTGTTGAAAGCCAAAGAGAGAATCTTGAAAAAGCAGTGAGAGAGAAGCGACTTGTTACATGCA
AGGGATCCTCAGTGAGATTATGAGCAGATCTCTCATCAGAAACTTTGGAGGCCAGGAGGCAGTGGGCTGATATATTCAAA
GTGCTAGAAGAAAACAATCTGTCAACCAAGAATCGTGTATCTGGCAAAACTGTCTTTCAAAAGTGAAGGAGAAATTAAGA
CATTTCCAGATAAACAAAAGCTGAGGGAGTTTCCTTACCACTAGACCTGTCTTGCAAGAAATGTTAAAGGGGCTG

Alignment

ORF - retro_ecab_858 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 50.62 %
• Parental protein coverage: 99.58 %
• Number of stop codons detected: 4
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  KELENIKKNQSEMKNTILEMKNSLEGLNSRVDDTEEWISKLDERLEEVIQAAQIKEKGIKKNKDSVRDLW
          ..LE...K...E.K..I.EMKNSLEG...R.....E...K...R....I.....KEK.............
Retrocopy ENLERSQKEILELKSIITEMKNSLEGFKGRFEQAKESVNKFEDRAIKIIESEGQKEKN*RIMNRAQATMR

Parental  NNIKHANIHIIRVPEGGERDKGTEKLFEEIIA-NFPNLRKGTDIQTQEAQRAPNKINPKRPTPRHIIIKM
          ...K..NIHI...PEG..R.KGTE.L.EE..A.NFP.L.K.T.I...EAQ..PN..N.KR.T..HI.IK.
Retrocopy HQQK*TNIHILGLPEGENREKGTERLSEELMAKNFPSLMKDTNINILEAQGIPNWMNSKRCTLSHINIKL

Parental  SVIKDKERFLKATRER-KQNLTSRGSSIRFSADFPSETLEARRQWAGIFKVLKEKN-CQPRILRPAKLPF
          ...K.KER.LK...ER.K...T..GSS.R..AD..SETLEARRQWA.IFKVL.E.N.CQPRI...AKL.F
Retrocopy --LKAKERILKKQ*ER<KRLVTCKGSSVRL*ADLSSETLEARRQWADIFKVLEENN>CQPRIVYLAKLSF

Parental  KSEGDIRTFPN--KLGEF-ITTRPTLRETLKGV
          KSEG.I.TFP...KL.EF..TTRP.L.E.LKG.
Retrocopy KSEGEIKTFPDKQKLREF>LTTRPVLQEMLKGL

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP021940_articular_cartilage	0.00 RPM	0.00 RPM
SRP021940_cerebellum	0.00 RPM	0.00 RPM
SRP021940_embryo	0.00 RPM	0.03 RPM
SRP021940_placental_villous	0.00 RPM	0.00 RPM
SRP021940_synovial_membrane	0.00 RPM	0.00 RPM
SRP021940_testis	0.00 RPM	0.00 RPM

ChIP-Seq

Equus caballus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ecab_858 retrocopy.

TSS

Equus caballus was not studied using FANTOM5 data.

Lab

retro_ecab_858 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ecab_858 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 5 parental genes, and 54 retrocopies.

Species	Parental gene accession	Retrocopies number
Equus caballus	ENSECAG00000010030	21 retrocopies	retro_ecab_1021, retro_ecab_1023, retro_ecab_1035, retro_ecab_1071, retro_ecab_1089, retro_ecab_136, retro_ecab_182, retro_ecab_201, retro_ecab_281, retro_ecab_306, retro_ecab_486, retro_ecab_521, retro_ecab_537, retro_ecab_582, retro_ecab_590, retro_ecab_658, retro_ecab_670, retro_ecab_722, retro_ecab_786, retro_ecab_974, retro_ecab_996,
Equus caballus	ENSECAG00000016527	5 retrocopies	retro_ecab_214, retro_ecab_642, retro_ecab_879, retro_ecab_888, retro_ecab_993,
Equus caballus	ENSECAG00000020332	9 retrocopies	retro_ecab_1018, retro_ecab_117, retro_ecab_125, retro_ecab_346, retro_ecab_54, retro_ecab_604, retro_ecab_774, retro_ecab_775, retro_ecab_878,
Equus caballus	ENSECAG00000022034	4 retrocopies	retro_ecab_1039, retro_ecab_278, retro_ecab_821, retro_ecab_858 ,
Equus caballus	ENSECAG00000023806	15 retrocopies	retro_ecab_1040, retro_ecab_1050, retro_ecab_1073, retro_ecab_1077, retro_ecab_1108, retro_ecab_176, retro_ecab_241, retro_ecab_298, retro_ecab_339, retro_ecab_714, retro_ecab_778, retro_ecab_781, retro_ecab_852, retro_ecab_855, retro_ecab_953,