RetrogeneDB ID: retro_mmus_2846

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 6:83600811..83601706(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Rpl5
• Ensembl ID: ENSMUSG00000058558
• Aliases: Rpl5, U21RNA
• Description: ribosomal protein L5 [Source:MGI Symbol;Acc:MGI:102854]

Sequence

>retro_mmus_2846
ATGGGGTTCATGAAAGTTGTCAAGAATAAGGCCTACTTTAAAAGACACCAAGTGAGATTTTGAAGGCGGCGAGAGGGTAA
AACTGACTACTATGCTCGGAAACGATTGGTGATCCAGGACAAGAATAAGTTCAACACACCCAAATATAGGATGGTAGTTC
GTGTAACTAACAGATATATCATCTGCCAGATTGCATATGCCCGTATAGAAGGGGATATGATCATCTGTGCAGCATATGCA
CATGAACTTCCAAAATATGGTCTGAAAGTTGGCCTGACAAATTATGCTGCAACCTATTGCACTGGCCTGCTGCTGGTCCC
CAGGCTTCTGAATAGGTTTGGCATGGACAAGATCTATGGAGGCCAAGTGGAGGTGAATGGAGGTGAATACAATGTGGAAA
GCATTGATGGTCAGCCTGGTGCCTTCACTTGCTATCTGGATGCAGGTCTTGCCCGAACTACAACTGGCAATAAAGTTTTG
GGGGCCCTTAAGGGAACTGTGGATGGAGACTTGTCTATCCCTCATAGTACCAAATGATCCCCTGGTTATGACTCTGAAAG
CAAGGAGTTCAATGCAGAGGTACATGCTCGGAAGCACACCATGGGTCAGGATGTGGCAGACTACATGCGCTACCTAATGG
AGGAAGATGAAGATGTGTATAAGAAACAGTTCTCTCAGTACATCAGGAACAACGTAACTCCAGACATGATGGAGGAGATG
TATAAGAAAGCTCATGCTGCTATCTGAGAGAATCCAGTCTATGAGAAGAAGCCCAAGAGAGAAGTGAAAAAGAAGAGGTG
GAATCGTCCCAAAATGTCTCTTGTGCAGAAAAAAAGATCGGGTTGCTCAAAAGAAGGCAAGCTTCCTCAGAGCTCAGGAA
AGGGCTGCTGAAAGC

Alignment

ORF - retro_mmus_2846 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 91.64 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 3
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MGFVKVVKNKAYFKRYQVRFRRRREGKTDYYARKRLVIQDKNKYNTPKYRMIVRVTNRDIICQIAYARIE
          MGF.KVVKNKAYFKR.QVRF.RRREGKTDYYARKRLVIQDKNK.NTPKYRM.VRVTNR.IICQIAYARIE
Retrocopy MGFMKVVKNKAYFKRHQVRF*RRREGKTDYYARKRLVIQDKNKFNTPKYRMVVRVTNRYIICQIAYARIE

Parental  GDMIVCAAYAHELPKYGVKVGLTNYAAAYCTGLLLARRLLNRFGMDKIYEGQVEVNGGEYNVESIDGQPG
          GDMI.CAAYAHELPKYG.KVGLTNYAA.YCTGLLL..RLLNRFGMDKIY.GQVEVNGGEYNVESIDGQPG
Retrocopy GDMIICAAYAHELPKYGLKVGLTNYAATYCTGLLLVPRLLNRFGMDKIYGGQVEVNGGEYNVESIDGQPG

Parental  AFTCYLDAGLARTTTGNKVFGALKGAVDGGLSIPHSTKRFPGYDSESKEFNAEVH-RKHIMGQNVADYMR
          AFTCYLDAGLARTTTGNKV.GALKG.VDG.LSIPHSTK..PGYDSESKEFNAEVH.RKH.MGQ.VADYMR
Retrocopy AFTCYLDAGLARTTTGNKVLGALKGTVDGDLSIPHSTK*SPGYDSESKEFNAEVHARKHTMGQDVADYMR

Parental  YLMEEDEDAYKKQFSQYIKNNVTPDMMEEMYKKAHAAIRENPVYEKKPKREVKKKRWNRPKMSLAQ-KKD
          YLMEEDED.YKKQFSQYI.NNVTPDMMEEMYKKAHAAI.ENPVYEKKPKREVKKKRWNRPKMSL.Q.KKD
Retrocopy YLMEEDEDVYKKQFSQYIRNNVTPDMMEEMYKKAHAAI*ENPVYEKKPKREVKKKRWNRPKMSLVQ>KKD

Parental  RVAQKKASFLRAQERAAES
          RVAQKKASFLRAQERAAES
Retrocopy RVAQKKASFLRAQERAAES

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.07 RPM	46.80 RPM
SRP007412_cerebellum	0.00 RPM	34.26 RPM
SRP007412_heart	0.09 RPM	57.28 RPM
SRP007412_kidney	0.15 RPM	78.35 RPM
SRP007412_liver	0.05 RPM	59.15 RPM
SRP007412_testis	0.05 RPM	29.04 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_2846 was not detected

EST

No EST(s) were mapped for retro_mmus_2846 retrocopy.

TSS

No TSS is located nearby retro_mmus_2846 retrocopy 5' end.

Lab

retro_mmus_2846 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_2846 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 16 parental genes, and 56 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000009878	5 retrocopies	retro_amel_1200, retro_amel_137, retro_amel_139, retro_amel_1439, retro_amel_1669,
Bos taurus	ENSBTAG00000002026	2 retrocopies	retro_btau_120, retro_btau_67,
Equus caballus	ENSECAG00000021065	1 retrocopy	retro_ecab_654,
Macropus eugenii	ENSMEUG00000006361	3 retrocopies	retro_meug_1168, retro_meug_1537, retro_meug_2146,
Monodelphis domestica	ENSMODG00000011635	1 retrocopy	retro_mdom_748,
Mustela putorius furo	ENSMPUG00000004150	6 retrocopies	retro_mputfur_213, retro_mputfur_449, retro_mputfur_451, retro_mputfur_597, retro_mputfur_721, retro_mputfur_833,
Mus musculus	ENSMUSG00000058558	11 retrocopies	retro_mmus_1000, retro_mmus_1185, retro_mmus_1338, retro_mmus_1789, retro_mmus_1991, retro_mmus_2577, retro_mmus_2846 , retro_mmus_297, retro_mmus_3076, retro_mmus_504, retro_mmus_911,
Ornithorhynchus anatinus	ENSOANG00000004669	1 retrocopy	retro_oana_97,
Oryctolagus cuniculus	ENSOCUG00000010089	2 retrocopies	retro_ocun_265, retro_ocun_54,
Petromyzon marinus	ENSPMAG00000002040	1 retrocopy	retro_pmar_23,
Pteropus vampyrus	ENSPVAG00000015117	4 retrocopies	retro_pvam_1427, retro_pvam_355, retro_pvam_505, retro_pvam_527,
Rattus norvegicus	ENSRNOG00000023529	6 retrocopies	retro_rnor_113, retro_rnor_2246, retro_rnor_2418, retro_rnor_2498, retro_rnor_2802, retro_rnor_2922,
Sarcophilus harrisii	ENSSHAG00000006417	1 retrocopy	retro_shar_202,
Sus scrofa	ENSSSCG00000006899	2 retrocopies	retro_sscr_149, retro_sscr_219,
Tupaia belangeri	ENSTBEG00000010560	5 retrocopies	retro_tbel_101, retro_tbel_1054, retro_tbel_1518, retro_tbel_3006, retro_tbel_3530,
Tursiops truncatus	ENSTTRG00000013097	5 retrocopies	retro_ttru_1446, retro_ttru_1548, retro_ttru_1796, retro_ttru_645, retro_ttru_979,