RetrogeneDB ID: retro_mmus_3368

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 9:95410781..95411612(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Trmt10a
• Ensembl ID: ENSMUSG00000004127
• Aliases: Trmt10a, 3110023L08Rik, AA794508, Rg9mtd2, Rnmtd2
• Description: tRNA methyltransferase 10A [Source:MGI Symbol;Acc:MGI:1920421]

Sequence

>retro_mmus_3368
CTAGGTGCAAGTGAAGGTGAAGAGGAGAGACAGAATCGAGAGTGGATGCAGGGGCAGAGCCAATCTCTAAACAACAACTG
AAGAAGCTGATGAAACAAAAACATTGGGAAGAGCAACAGGAACAGCGCAAGGAAAAAGAAAAAGGCAAGAGGAAGAAATT
AGAACGCCGGCCACCTGGAGTCCAACTCAGATGGAAACTACAGGAAGCATATTTGAAGGGATGTTGCTCGTAGCAGCCTC
CTCCTGATCATTGACTGTAGCTTTGATGACTTGATGGTGTTAAAGGATATTAAGAAACTTCATAAACAGATTCAATGATG
TTATGCAGAAACCTGCCGAGCATCTCATCCAGTGCCATCTTACTTGACAAGCCATGGAGGTCAACTGAAGAAGAACATGG
ATGAGAACGACCAAGGATGGGTCAACTGGAAGGATATTCACATCAAATCAGAGCACCATAGTGAACTGTTAAAAAAAAAA
AAAGACCTGGTTTATCTGACATCGGACTCACCTAATGTACTGAAGGACTTAGATAAGTCAAAGGCCTATGTGATTGGAGG
GCTAGTGGACCACAACCATCACCAGAGACATGCATTTAAGCAAGCATCCAGTTATGGAATCGAACACACACAGCTCCCTC
TTGCAGATTTTGTGAAGATGAATAGTCGAAAAGTCCTGGCAGCTAACCATGTGTTTGAAATTATCCTGGAGCTCTTGGAA
ACAAGAGACTGGCAAGAGGTGTTTTTTTTTTTTTTTTTTTTTTTTTACAATCTTACCCCAACGGAAAGGAGCCGTTCCTG
CACACAAGACCTGCGAAAGCTCTCCTCAGGA

Alignment

ORF - retro_mmus_3368 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 77.3 %
• Parental protein coverage: 84.55 %
• Number of stop codons detected: 2
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  LGTSDGEEERQ-EPRVDAGAEPISKRQLKKLMKQKQWEEQREQRKEKRKEKRKRKKLERR-QLESNSDGN
          LG.S.GEEERQ.E.RVDAGAEPISK.QLKKLMKQK.WEEQ.EQRKEK..EK.KRKKLERR..LESNSDGN
Retrocopy LGASEGEEERQ<ESRVDAGAEPISKQQLKKLMKQKHWEEQQEQRKEK--EKGKRKKLERR>HLESNSDGN

Parental  DRKRVRRDVARSSLRLVIDCSFDDLMVLKDIKKLHKQIQRCYAENRRASHPVQFYLTSHGGQLKKNMDEN
          .RK...RDVARSSL.L.IDCSFDDLMVLKDIKKLHKQIQ.CYAE..RASHPV..YLTSHGGQLKKNMDEN
Retrocopy YRKHI*RDVARSSLLLIIDCSFDDLMVLKDIKKLHKQIQ*CYAETCRASHPVPSYLTSHGGQLKKNMDEN

Parental  DQGWVNWKDIHIKSEHYSEL-IKKEDLVYLTSDSPNVLKDLDESKAYVIGGLVDHNHHKGLTFKQATSYG
          DQGWVNWKDIHIKSEH.SEL.....DLVYLTSDSPNVLKDLD.SKAYVIGGLVDHNHH....FKQA.SYG
Retrocopy DQGWVNWKDIHIKSEHHSELXXXXXDLVYLTSDSPNVLKDLDKSKAYVIGGLVDHNHHQRHAFKQASSYG

Parental  IEHAQLPLADFVKMNSRKVLAVNHVFEIILEFLETRDWQEAFFTILPQRKGAVPAHKACESSPQDHQSLP
          IEH.QLPLADFVKMNSRKVLA.NHVFEIILE.LETRDWQE.............P......S..QD...L.
Retrocopy IEHTQLPLADFVKMNSRKVLAANHVFEIILELLETRDWQEVXXXXXXXXXNLTPTERS-RSCTQDLRKLS

Parental  EG
          .G
Retrocopy SG

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.05 RPM	5.89 RPM
SRP007412_cerebellum	0.04 RPM	4.86 RPM
SRP007412_heart	0.00 RPM	1.81 RPM
SRP007412_kidney	0.02 RPM	4.31 RPM
SRP007412_liver	0.00 RPM	4.34 RPM
SRP007412_testis	0.00 RPM	66.64 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_3368 was not detected

EST

No EST(s) were mapped for retro_mmus_3368 retrocopy.

TSS

No TSS is located nearby retro_mmus_3368 retrocopy 5' end.

Lab

retro_mmus_3368 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_3368 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 6 parental genes, and 9 retrocopies.

Species	Parental gene accession	Retrocopies number
Ailuropoda melanoleuca	ENSAMEG00000001076	1 retrocopy	retro_amel_1868,
Macaca mulatta	ENSMMUG00000009090	1 retrocopy	retro_mmul_2024,
Mus musculus	ENSMUSG00000004127	3 retrocopies	retro_mmus_3007, retro_mmus_3008, retro_mmus_3368 ,
Oryctolagus cuniculus	ENSOCUG00000015213	1 retrocopy	retro_ocun_1482,
Otolemur garnettii	ENSOGAG00000005330	2 retrocopies	retro_ogar_2061, retro_ogar_3335,
Ictidomys tridecemlineatus	ENSSTOG00000005064	1 retrocopy	retro_itri_1131,