RetrogeneDB ID: retro_mmus_520

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 1:191001427..191001851(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Rps15
• Ensembl ID: ENSMUSG00000063457
• Aliases: Rps15, rig
• Description: ribosomal protein S15 [Source:MGI Symbol;Acc:MGI:98117]

Sequence

>retro_mmus_520
ATGGTTGAAGTAGAGCAGAAGAAGTGAACCTTCTGCAAGTTAACTTATGGTGGCGTGTACCTGGACCAGCTGCTGGACAT
GTCCTGTGAGCAGCTGAAACAGTTAAGACAGCACCTGCCAGAAGCAGTACCTGAACTGTGGCCTGCTTCGGAAGCAGCGC
TCACTACTCAGGCACTTGAGAAAGGCCAAGAAGGAGGCACCACCCCTGGAGAAGCCCGAGGTGGTGAAGACGCACCTGAG
GGGCATGATCATGCTGCCAGAGATGGTAGGCAGCATGGTGGGCATGTACAACAGCAAGACCTTTCAACCAGGTGGAGATC
AAACCGAGTTGATCGGCTACTACCTGGATGAGTTCTCCATCCCCTACAAGCCGATGAAGTCTGGTATTGGTACCACCCAT
GGCTCCCACTTCATCCCCCTGAAA

Alignment

ORF - retro_mmus_520 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 72.97 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 3

Retrocopy - Parental Gene Alignment:

Parental  MAEVEQKKKRTFRKFTYRGVDLDQLLDMSYEQLMQLY-SARQRRRLNRGLRRKQHSLLKRLRKAKKEAPP
          M.EVEQKK..TF.K.TY.GV.LDQLLDMS.EQL.QL..S..Q...LN.GL.RKQ.SLL..LRKAKKEAPP
Retrocopy MVEVEQKK*-TFCKLTYGGVYLDQLLDMSCEQLKQLR>STCQKQYLNCGLLRKQRSLLRHLRKAKKEAPP

Parental  MEKPEVVKTHLRDMIILPEMVGSMVGVYNGKT-FNQVEIKP-EMIGHYLGEFSITYKPVKHGRPGIGATH
          .EKPEVVKTHLR.MI.LPEMVGSMVG.YN.KT.FNQVEIKP.E.IG.YL.EFSI.YKP.K.G...IG.TH
Retrocopy LEKPEVVKTHLRGMIMLPEMVGSMVGMYNSKT>FNQVEIKP<ELIGYYLDEFSIPYKPMKSG---IGTTH

Parental  SSRFIPLK
          .S.FIPLK
Retrocopy GSHFIPLK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	1.33 RPM	129.06 RPM
SRP007412_cerebellum	0.56 RPM	104.25 RPM
SRP007412_heart	0.00 RPM	177.26 RPM
SRP007412_kidney	0.17 RPM	132.61 RPM
SRP007412_liver	0.00 RPM	112.76 RPM
SRP007412_testis	0.05 RPM	82.60 RPM

ChIP-Seq

RNA Polymerase II activity near the 5' end of retro_mmus_520 was not detected

EST

No EST(s) were mapped for retro_mmus_520 retrocopy.

TSS

No TSS is located nearby retro_mmus_520 retrocopy 5' end.

Lab

retro_mmus_520 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_520 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 20 parental genes, and 69 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000019718	1 retrocopy	retro_btau_1856,
Callithrix jacchus	ENSCJAG00000005632	13 retrocopies	retro_cjac_143, retro_cjac_151, retro_cjac_1517, retro_cjac_1607, retro_cjac_1672, retro_cjac_1714, retro_cjac_2069, retro_cjac_2236, retro_cjac_2596, retro_cjac_2656, retro_cjac_48, retro_cjac_756, retro_cjac_86,
Cavia porcellus	ENSCPOG00000003968	1 retrocopy	retro_cpor_495,
Dasypus novemcinctus	ENSDNOG00000000706	2 retrocopies	retro_dnov_173, retro_dnov_85,
Echinops telfairi	ENSETEG00000008645	1 retrocopy	retro_etel_2,
Felis catus	ENSFCAG00000010769	1 retrocopy	retro_fcat_1570,
Homo sapiens	ENSG00000115268	4 retrocopies	retro_hsap_1484, retro_hsap_2341, retro_hsap_2650, retro_hsap_530,
Gorilla gorilla	ENSGGOG00000002818	4 retrocopies	retro_ggor_1136, retro_ggor_116, retro_ggor_141, retro_ggor_1851,
Macaca mulatta	ENSMMUG00000003050	2 retrocopies	retro_mmul_2130, retro_mmul_838,
Monodelphis domestica	ENSMODG00000005119	1 retrocopy	retro_mdom_1335,
Mus musculus	ENSMUSG00000063457	8 retrocopies	retro_mmus_1270, retro_mmus_208, retro_mmus_2734, retro_mmus_2786, retro_mmus_3166, retro_mmus_3642, retro_mmus_520 , retro_mmus_917,
Nomascus leucogenys	ENSNLEG00000001651	8 retrocopies	retro_nleu_105, retro_nleu_1858, retro_nleu_1922, retro_nleu_2390, retro_nleu_2465, retro_nleu_2559, retro_nleu_461, retro_nleu_544,
Otolemur garnettii	ENSOGAG00000015776	4 retrocopies	retro_ogar_194, retro_ogar_210, retro_ogar_2237, retro_ogar_96,
Pongo abelii	ENSPPYG00000009317	4 retrocopies	retro_pabe_1233, retro_pabe_2321, retro_pabe_67, retro_pabe_893,
Pan troglodytes	ENSPTRG00000010200	1 retrocopy	retro_ptro_122,
Pteropus vampyrus	ENSPVAG00000003168	2 retrocopies	retro_pvam_382, retro_pvam_926,
Rattus norvegicus	ENSRNOG00000024603	8 retrocopies	retro_rnor_1384, retro_rnor_1711, retro_rnor_1712, retro_rnor_178, retro_rnor_2086, retro_rnor_2114, retro_rnor_2642, retro_rnor_700,
Sarcophilus harrisii	ENSSHAG00000013424	1 retrocopy	retro_shar_632,
Sus scrofa	ENSSSCG00000023248	1 retrocopy	retro_sscr_271,
Ictidomys tridecemlineatus	ENSSTOG00000006423	2 retrocopies	retro_itri_722, retro_itri_939,