RetrogeneDB ID: retro_mmus_1987

Retrocopy location

• Organism: Mouse (Mus musculus)
• Coordinates: 2:151458955..151460306(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: ENSMUSG00000082809
• Aliases: None
• Status: KNOWN_PSEUDOGENE

Parental gene information

• Parental gene symbol: Tuba1b
• Ensembl ID: ENSMUSG00000023004
• Aliases: Tuba1b, Tuba2
• Description: tubulin, alpha 1B [Source:MGI Symbol;Acc:MGI:107804]

Sequence

>retro_mmus_1987
ATGCGTGAGTGCATCTCCATCCACGTTGGCCAGACTGGTGTCCAGATCGGCAATGCCTGCTGGGAGCTCTACTGCCTGGA
ACATGGCATCCAGCCTGATGGCCAGATGCCAAGTGACAAGACCATTGGGGGAGGAGATGACTCCTTCAACACCTTCTTCA
GTGAGACAGGAGCTGGCAAGCATGTGCCCCGGGCAGTGTTTGTAGACCTGGAACCCACGGTCATCGATGAAGTTCGCACT
GGCACCTACCGCCAGCTCTTCCACCCTGAGCAGCTCATCACAGGCAAGGAGGATGCTGCCAATAACTATGCCCGTGGCCA
CTACACCATTGGCAAGGAGATCATTGACCTTGTCCTGGACAGGATTCGCAAGCTGGCTGACCAGTGCACAGGTCTCCAGG
GCTTCTTGGTTTTCCACAGCTTTGGGGGAGAACTGGCTCTGGCTTCACCTCCCTGCTGATGGAGCGGCTCTCTGTGGATT
ACGGAAAGAAGTCCAAGCTGGAGTTCTCCATTTACCCAGGCCCCAGGTTTCCACTGCTGTGGTTGATCCCTACAATTCTA
TCCTCACCACCCATACCACTCTGGAGCACTCTGATTGTGCCTTCATGGTAGACAATGAGGCCATCTATGACATCTGTCGT
AGAAACCTCGACATTGAGCGCCCAACCTACACCAACCTTAACCGCCTTATTAGCCAGATTGTGTCTTCCATCACTGCTTC
CCTCAGATTTGATGGGGCCCTGAATGTTGATCTGACAGAATTCCAGACCAACCTGGTACCCTACCCTCGCATCCACTTCC
CTCTGGCCACTTATGCCCCTGTCATCTCTGCTGAGAAAGCCTACCATGAGCAGCTTTCTGTAGCAGAGATCACCAATGCC
TGCTTTGAGCCAGCCAACCAGATGGTGAAATGTGACCCTCGCCATGGTAAATACATGGCTTGCTGCCTGCTGTACCGTGG
TGATGTGGTTCCCAAAGATGTCAATGCTGCCATTGCCACCATCAAGACCAAGCGCAGCATCCAGTTTGTAGACTGGTGCC
CCACTGGCTTCAAGGTTGGCATTAATTACCAGCCTCCCACTGTGGTACCCGGTGGTGACCTGGCCAAGGTGCAGAGAGCT
GTGTGCATGCTGAGCAACACCACAGCCACTGCTGAGGCCTGGGCTCGCCTAGATCACAAGTTTGATCTGATGTATGCCAA
GCGTGCCTTTGTGCACTGGTATGTGGGTGAGGGCATGGAGGAGGGTGAGTTCTCTGAGGCCCGTGAGGACATGACTGCCC
TAGAGAAGGATTATGAGGAGGTTGGTGTGGATTCTGTGGAAGGCAAGGGGGAGGAAAAAGAAGAGGAATAC

Alignment

ORF - retro_mmus_1987 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 97.57 %
• Parental protein coverage: 100. %
• Number of stop codons detected: 0
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
          MRECISIHVGQ.GVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
Retrocopy MRECISIHVGQTGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL

Parental  EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
          EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
Retrocopy EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS

Parental  FGGG-TGSGFTSLLMERLSVDYGKKSKLEFSIYPAP-QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEA
          FGG..TGSGFTSLLMERLSVDYGKKSKLEFSIYP.P.QVSTAVV.PYNSILTTHTTLEHSDCAFMVDNEA
Retrocopy FGGE<TGSGFTSLLMERLSVDYGKKSKLEFSIYPGP<QVSTAVVDPYNSILTTHTTLEHSDCAFMVDNEA

Parental  IYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISA
          IYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISA
Retrocopy IYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISA

Parental  EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCP
          EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCP
Retrocopy EKAYHEQLSVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCP

Parental  TGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEF
          TGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTA.AEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEF
Retrocopy TGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTATAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEF

Parental  SEAREDMAALEKDYEEVGVDSVEGEGEEEGEEY
          SEAREDM.ALEKDYEEVGVDSVEG.GEE..EEY
Retrocopy SEAREDMTALEKDYEEVGVDSVEGKGEEKEEEY

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.23 RPM	77.45 RPM
SRP007412_cerebellum	0.17 RPM	63.92 RPM
SRP007412_heart	0.22 RPM	36.14 RPM
SRP007412_kidney	0.04 RPM	16.90 RPM
SRP007412_liver	0.05 RPM	8.82 RPM
SRP007412_testis	4.12 RPM	8.14 RPM

ChIP-Seq

RNA Polymerase II actvity near the 5' end of retro_mmus_1987 was not detected

EST

No EST(s) were mapped for retro_mmus_1987 retrocopy.

TSS

TSS No.	TSS Name	TSS expression level (Expr) in TPM range:
		no expression	0 < Expr ≤ 1	1 < Expr ≤ 5	5 < Expr ≤ 10	Expr > 10
TSS #1	TSS_81440	77 libraries	100 libraries	526 libraries	253 libraries	116 libraries

The graphical summary, for retro_mmus_1987 TSS expression levels > 0 TPM .
TSS expression levels were studied across 1072 TSS-CAGE libraries, based on FANTOM5 data.
The expression values were visualized using beanplot. If you have any doubts, how to read it, read more in Kampstra P (2008)

Lab

retro_mmus_1987 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmus_1987 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 14 parental genes, and 29 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000012244	1 retrocopy	retro_btau_402,
Choloepus hoffmanni	ENSCHOG00000008444	2 retrocopies	retro_chof_179, retro_chof_360,
Callithrix jacchus	ENSCJAG00000020874	2 retrocopies	retro_cjac_1603, retro_cjac_1861,
Felis catus	ENSFCAG00000028689	3 retrocopies	retro_fcat_1802, retro_fcat_254, retro_fcat_798,
Homo sapiens	ENSG00000123416	1 retrocopy	retro_hsap_808,
Macropus eugenii	ENSMEUG00000010779	2 retrocopies	retro_meug_1648, retro_meug_2174,
Macropus eugenii	ENSMEUG00000011167	4 retrocopies	retro_meug_1121, retro_meug_157, retro_meug_1616, retro_meug_1964,
Monodelphis domestica	ENSMODG00000015206	2 retrocopies	retro_mdom_1632, retro_mdom_348,
Mus musculus	ENSMUSG00000023004	6 retrocopies	retro_mmus_1562, retro_mmus_1607, retro_mmus_1742, retro_mmus_1987 , retro_mmus_3297, retro_mmus_3327,
Mus musculus	ENSMUSG00000043091	1 retrocopy	retro_mmus_843,
Mus musculus	ENSMUSG00000067338	1 retrocopy	retro_mmus_3356,
Otolemur garnettii	ENSOGAG00000009435	1 retrocopy	retro_ogar_240,
Pan troglodytes	ENSPTRG00000012950	1 retrocopy	retro_ptro_2158,
Sus scrofa	ENSSSCG00000000190	2 retrocopies	retro_sscr_380, retro_sscr_702,