RetrogeneDB ID: retro_ocun_1771

Retrocopy location

• Organism: Rabbit (Oryctolagus cuniculus)
• Coordinates: GL018880:111383..112695(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: EIF2A
• Ensembl ID: ENSOCUG00000016095
• Aliases: None
• Description: eukaryotic translation initiation factor 2A, 65kDa [Source:HGNC Symbol;Acc:3254]

Sequence

>retro_ocun_1771
AATGTTAACCATGAAGTTCACTTCTTTGAAAAGAACAATTTCAACATAATTGCAAATAAATTACATTTGCAGAAAATTAA
TGATTTGGTGTTATCACCTGGACCCCAGCCATACAAGGTGGCTGTCTATGTTCCAGGAAGCAAAGGTGCACCTTCGTATG
TTAGATTGTATCAGTACCCCAACTTTGGTGGACCTCATGCAGCTTTAGCCAATAAAAGTTTCTTTAAGGCTGATAAGGTT
ACAATGCTATGGAATAAAAAAGCTACTGCGGTCTTGGTAATAGCTAGTAGAGACATTGATAAGACAGGAGCTTCCTATTA
TGGGGAATAGATGTTGCATTACATTGCAACAAATGGAGAAAGTGCCATAGTGCAACTACCAAAAGGTGGCCCTATTTATG
ACATAGTTTGGAATTATAGTTCTTCTGAATTTTGTGCTGTTTATGGTTTTAAGCCTGCCAAAGCAACACTTTTCAACTTG
AAATGTGATCCTGTGTTTGACTTTGGAACTGGTCCTCAGAATGCAGGCTACTACAGCCCTCAAGGACATATATTATTACT
AGCTGGATTGGGAAATCTGAGAGGACAAATGGAAGTATGGGATGTTAAAAACCACAAGGTCATTTCTAAACCAGTGGCTT
CTGATTCTACATATTTTGCTGCATGCCTAGATGGTGAGCATATTTTACCAGCCACATGTGCTCCCAGGTTACGTGTTAAT
AATGGGTATGAGATTTGGCATTATGCTGGCTCTGTATTGCACAAGTATGATGTGCCCTCAAGTGGAGACCTATGGCTGGT
TTCATGGCAGCCATTTTTGGATGGAATATTTCCAGCAAAAGCAGTAACTTACCAAGCAGTTCCAAGTGAAGGAACCTAAA
GTTGCAACTGTGTATAGACCCCCTGCTTTAAGAAACAAGCCAGTCACCAATTCCAAGCTGCATGAATAGGAGCCACCTCA
GAATATGAAACCAAAATCAGGAAATGATAAGCACCCATCAAAAACAACCCTTAAAAATCAAAGGAAGCACGAAGCTAAGA
ACGCTGCAAAGTAGGAAGCAAGAAGTGACAAAAGTCCAGATTTGCCCTCTACTCCTGTGCCACAAAACACACCATGAAAC
ATTACCTCTCGGTCAACTTCTGGAGACCCTGAGGTAGACAAAAAAATCTAGGACCTAAAGTAGAAACTGAAAGCAATTGA
ACAACTGAAAGGACAAGCAGCAAGTGGAAAACAGCTAGAAAAAAGTCCGTTGGACAACATTCAAAAAGAGACAGCCCTTC
TTCAGGAGCTGGAAGATGTGGAATTGGGTGTT

Alignment

ORF - retro_ocun_1771 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 86.88 %
• Parental protein coverage: 75.38 %
• Number of stop codons detected: 6
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  NVNNEVHFFENNNFNIIANKLHLQKINDFVLSPGPQPYKVAVYVPGSKGAPSFVRLYQYPNFGGPHAALA
          NVN.EVHFFE.NNFNIIANKLHLQKIND.VLSPGPQPYKVAVYVPGSKGAPS.VRLYQYPNFGGPHAALA
Retrocopy NVNHEVHFFEKNNFNIIANKLHLQKINDLVLSPGPQPYKVAVYVPGSKGAPSYVRLYQYPNFGGPHAALA

Parental  NKSFFKADKVTMLWNKKATAVLVIASTDVDKTGASYYGEQTLHYIATNGESAVVQLPKGGPIYDVAWNSS
          NKSFFKADKVTMLWNKKATAVLVIAS.D.DKTGASYYGE..LHYIATNGESA.VQLPKGGPIYD..WN.S
Retrocopy NKSFFKADKVTMLWNKKATAVLVIASRDIDKTGASYYGE*MLHYIATNGESAIVQLPKGGPIYDIVWNYS

Parental  STEFCAVYGFMPAKATIFNLKCDPVFDFGTGPRNAAYYSPQGHILVLAGFGNLRGQMEVWDVKNYKLISK
          S.EFCAVYGF.PAKAT.FNLKCDPVFDFGTGP.NA.YYSPQGHIL.LAG.GNLRGQMEVWDVKN.K.ISK
Retrocopy SSEFCAVYGFKPAKATLFNLKCDPVFDFGTGPQNAGYYSPQGHILLLAGLGNLRGQMEVWDVKNHKVISK

Parental  PVASDSTYFAWCPDGEHILTATCAPRLRVNNGYKIWHYTGSVLHKYDVPSNGELWQVSWQPFLDGIFPAK
          PVASDSTYFA.C.DGEHIL.ATCAPRLRVNNGY.IWHY.GSVLHKYDVPS.G.LW.VSWQPFLDGIFPAK
Retrocopy PVASDSTYFAACLDGEHILPATCAPRLRVNNGYEIWHYAGSVLHKYDVPSSGDLWLVSWQPFLDGIFPAK

Parental  AITYQAVPSEVPSE-EPKVATAYRPPALRNKPVTNSKLHEEEPPQNMKPQSGSDKPLSKTALKNQRKHEA
          A.TYQA....VPSE.EPKVAT.YRPPALRNKPVTNSKLHE.EPPQNMKP.SG.DK..SKT.LKNQRKHEA
Retrocopy AVTYQA----VPSE>EPKVATVYRPPALRNKPVTNSKLHE*EPPQNMKPKSGNDKHPSKTTLKNQRKHEA

Parental  KKAAKQEARSDKSPDLPSTPAPQSTPRNITSQSTSGDPEVDKKIKNLKKKLKAIEQLKEQAASGKQLEKN
          K.AAK.EARSDKSPDLPSTP.PQ.TP.NITS.STSGDPEVDKKI..LK.KLKAIEQLK.QAASGKQLEK.
Retrocopy KNAAK*EARSDKSPDLPSTPVPQNTP*NITSRSTSGDPEVDKKI*DLK*KLKAIEQLKGQAASGKQLEKS

Parental  QLEKIQKETALLQELEDLELGI
          .L..IQKETALLQELED.ELG.
Retrocopy PLDNIQKETALLQELEDVELGV

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP017611_brain	0.00 RPM	39.24 RPM
SRP017611_kidney	0.00 RPM	42.47 RPM
SRP017611_liver	0.00 RPM	16.42 RPM

ChIP-Seq

Oryctolagus cuniculus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_ocun_1771 retrocopy.

TSS

Oryctolagus cuniculus was not studied using FANTOM5 data.

Lab

retro_ocun_1771 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_ocun_1771 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 12 parental genes, and 15 retrocopies.

Species	Parental gene accession	Retrocopies number
Callithrix jacchus	ENSCJAG00000001199	1 retrocopy	retro_cjac_968,
Dasypus novemcinctus	ENSDNOG00000014002	1 retrocopy	retro_dnov_1148,
Homo sapiens	ENSG00000144895	1 retrocopy	retro_hsap_565,
Gorilla gorilla	ENSGGOG00000015391	1 retrocopy	retro_ggor_509,
Loxodonta africana	ENSLAFG00000003767	1 retrocopy	retro_lafr_502,
Macaca mulatta	ENSMMUG00000017696	1 retrocopy	retro_mmul_2395,
Monodelphis domestica	ENSMODG00000028858	1 retrocopy	retro_mdom_618,
Nomascus leucogenys	ENSNLEG00000003219	1 retrocopy	retro_nleu_2213,
Oryctolagus cuniculus	ENSOCUG00000016095	3 retrocopies	retro_ocun_1771 , retro_ocun_1825, retro_ocun_1861,
Pongo abelii	ENSPPYG00000029636	2 retrocopies	retro_pabe_517, retro_pabe_652,
Pan troglodytes	ENSPTRG00000015528	1 retrocopy	retro_ptro_423,
Ictidomys tridecemlineatus	ENSSTOG00000003374	1 retrocopy	retro_itri_1706,