RetrogeneDB ID: retro_mmul_1721

Retrocopy location

• Organism: Rhesus macaque (Macaca mulatta)
• Coordinates: 3:128632009..128633395(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: MMU.11091
• Ensembl ID: ENSMMUG00000004852
• Aliases: None
• Description: eukaryotic translation elongation factor 1 alpha 1 [Source:RefSeq peptide;Acc:NP_001182679]

Sequence

>retro_mmul_1721
ATGGAAGAGGAACACACTCATAACAGCATTGTTGTCATGGGACATGTAGATGGGGCAAGTCCACCACTACTGGCCATCTG
ATCCACAAATGAAGTGGCATCGACAAAAGAACCATTGAAAATTTTGAGAAGGAGACTGCTGAGATGGGAACGGGCTCATT
CAAGTATGCCTGGGTCTTGGATAAACTGAAAGCTGAACGTGAACGTGGTGTCACCATTGATATCTCCCTGCCAAAATTTG
AGACCAGCAAATACTACGTGACTACCATTGATTCCCCAGGACACAGACACTTTATCAAAAACATGATTATAGGGACATCT
CAGGCTGACCATGCTGTCCTGATTGTTGCTGCTGGTGTTGCTGAATTTGAAGCTGGAACCTCCAAGAATGGGTGGACCCA
TGAGCATGCTTTTCTGGCTTACACACTGGGTGGGAAACAACTAATTGTTGATGTTAGCAAAAGGGATCCATTTGAACGAT
CCTAAAACCAGAAGAAATACGAAGAAATAATTAAGGAAGTCAGCACTTACCTTAAGAAAATTAACTACAGCCCTGACACA
GTAGCATTTGTGCCAGTTTCTGGTTGGAATGGTGACAACATACTGGAGCCAAGTGCTAGCATGCCTAGGTTCAAGGGATG
GAAAGTCACTCGGAAAGATGGCAATGCAAGTGGAACCAAACTGCTTGAGGCTCTGGACTGCATCCTACCACCAGCTTGTC
CAACTGACAAGCCCTTGCCCCTGCCTCTCCAGGATGTCTACAAAATTGGTGGTATTGCTACTCTGTCTATTGGTGGAGTG
GAGACTGGTGTTCTCAAACCTGGTATGGTGATCACTTTTGTTCCAGTCAATGTTACAACTGAAGTAAAGTCTGTTGAAAT
ACACCATGAAGTTTTGAGTGACTCTCTTCCTCAGAACAATGTGGCCTTCAATGTCAAGAATGTGTCTGTCAAAAATGTTC
ATTGTGGCATTGTTGCTGGTGACAACAAAAATGATTCATCAACAGAAACAACTGACTTCACTGCTCAGGTGATTATCCTG
AATCATCCAGTCTAAATCAGTGCTGGCCATTCCCCTGTACTGGATTGCCACACAGCTCACATTGCATGCAAATTTGCCGA
GCTGAAAGAAAAGATTGATCGCCATTCTGGTCAGAAGCTGAAAGATGGTCCTAAATTCTTGAAGTCTGGTGATGTTGCCA
TCAGTTGATATGGTTCTTGGCAAGCCAATGTGTGTTGAGAGCTTCTCAGACTATCCTCCTTTGAGTTGCTTTGCTGTTCA
TGATATGAGACGGAGAGTTGCCATGGGTGTCATCAAAGCAGTGGACAAGAAGGCTGCTGGAGCTGGCAAGGTCACCAAGT
CTGCCCAGAGAGTTCAGAAGGCTAAA

Alignment

ORF - retro_mmul_1721 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 80.17 %
• Parental protein coverage: 100.0 %
• Number of stop codons detected: 3
• Number of frameshifts detected: 2

Retrocopy - Parental Gene Alignment:

Parental  MGKEKTHINIVVIGHVDS-GKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERER
          M..E.TH..IVV.GHVD..GKSTTTGHLI.K..GIDKRTIE.FEKE.AEMG.GSFKYAWVLDKLKAERER
Retrocopy MEEEHTHNSIVVMGHVDG<GKSTTTGHLIHK*SGIDKRTIENFEKETAEMGTGSFKYAWVLDKLKAERER

Parental  GITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALL
          G.TIDISL.KFETSKYYVT.ID.PGHR.FIKNMI.GTSQAD.AVLIVAAGV.EFEAG.SKNG.T.EHA.L
Retrocopy GVTIDISLPKFETSKYYVTTIDSPGHRHFIKNMIIGTSQADHAVLIVAAGVAEFEAGTSKNGWTHEHAFL

Parental  AYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMP
          AYTLG.KQLIV.V.K.D..E....QK.YEEI.KEVSTY.KKI.Y.PDTVAFVP.SGWNGDN.LEPSA.MP
Retrocopy AYTLGGKQLIVDVSKRDPFERS*NQKKYEEIIKEVSTYLKKINYSPDTVAFVPVSGWNGDNILEPSASMP

Parental  WFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVT
          .FKGWKVTRKDGNASGT.LLEALDCILPP..PTDKPL.LPLQDVYKIGGI.T...G.VETGVLKPGMV.T
Retrocopy RFKGWKVTRKDGNASGTKLLEALDCILPPACPTDKPLPLPLQDVYKIGGIATLSIGGVETGVLKPGMVIT

Parental  FAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNH
          F.PVNVTTEVKSVE.HHE.LS..LP..NV.FNVKNVSVK.V..G.VAGD.KND...E...FTAQVIILNH
Retrocopy FVPVNVTTEVKSVEIHHEVLSDSLPQNNVAFNVKNVSVKNVHCGIVAGDNKNDSSTETTDFTAQVIILNH

Parental  PGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAI-VDMVPGKPMCVESFSDY
          P..ISAG..PVLDCHTAHIACKFAELKEKIDR.SG.KL.DGPKFLKSGD.AI.VDMV.GKPMCVESFSDY
Retrocopy PV*ISAGHSPVLDCHTAHIACKFAELKEKIDRHSGQKLKDGPKFLKSGDVAI>VDMVLGKPMCVESFSDY

Parental  PPLGRFAVRDMRQTVAVGVIKAVDKKAAGAGKVTKSAQKAQKAK
          PPL..FAV.DMR..VA.GVIKAVDKKAAGAGKVTKSAQ..QKAK
Retrocopy PPLSCFAVHDMRRRVAMGVIKAVDKKAAGAGKVTKSAQRVQKAK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain	0.00 RPM	257.72 RPM
SRP007412_brain_prefrontal_cortex	0.00 RPM	933.00 RPM
SRP007412_cerebellum	0.06 RPM	305.24 RPM
SRP007412_heart	0.06 RPM	637.02 RPM
SRP007412_kidney	0.00 RPM	964.42 RPM
SRP007412_liver	0.04 RPM	1738.88 RPM
SRP007412_testis	0.08 RPM	580.33 RPM

ChIP-Seq

Macaca mulatta was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_mmul_1721 retrocopy.

TSS

Macaca mulatta was not studied using FANTOM5 data.

Lab

retro_mmul_1721 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_mmul_1721 has 2 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_3758
Gorilla gorilla	retro_ggor_2526

---

Parental genes homology:

Parental genes homology involve 13 parental genes, and 68 retrocopies.

Species	Parental gene accession	Retrocopies number
Canis familiaris	ENSCAFG00000013249	1 retrocopy	retro_cfam_1007,
Callithrix jacchus	ENSCJAG00000010051	6 retrocopies	retro_cjac_1431, retro_cjac_3075, retro_cjac_34, retro_cjac_3735, retro_cjac_733, retro_cjac_97,
Cavia porcellus	ENSCPOG00000004067	15 retrocopies	retro_cpor_1150, retro_cpor_1162, retro_cpor_1191, retro_cpor_1256, retro_cpor_1393, retro_cpor_1492, retro_cpor_1499, retro_cpor_348, retro_cpor_381, retro_cpor_387, retro_cpor_413, retro_cpor_709, retro_cpor_891, retro_cpor_918, retro_cpor_959,
Dipodomys ordii	ENSDORG00000009629	1 retrocopy	retro_dord_509,
Equus caballus	ENSECAG00000022260	1 retrocopy	retro_ecab_802,
Erinaceus europaeus	ENSEEUG00000000342	3 retrocopies	retro_eeur_407, retro_eeur_492, retro_eeur_625,
Loxodonta africana	ENSLAFG00000028081	1 retrocopy	retro_lafr_651,
Macaca mulatta	ENSMMUG00000004852	15 retrocopies	retro_mmul_1375, retro_mmul_1407, retro_mmul_1518, retro_mmul_1525, retro_mmul_1690, retro_mmul_1707, retro_mmul_1721 , retro_mmul_1739, retro_mmul_1743, retro_mmul_1906, retro_mmul_2196, retro_mmul_2250, retro_mmul_323, retro_mmul_6, retro_mmul_848,
Monodelphis domestica	ENSMODG00000018533	4 retrocopies	retro_mdom_1053, retro_mdom_1146, retro_mdom_311, retro_mdom_792,
Mus musculus	ENSMUSG00000037742	15 retrocopies	retro_mmus_1349, retro_mmus_1518, retro_mmus_1667, retro_mmus_1783, retro_mmus_1816, retro_mmus_1849, retro_mmus_1850, retro_mmus_1864, retro_mmus_1909, retro_mmus_2363, retro_mmus_2583, retro_mmus_2902, retro_mmus_3249, retro_mmus_480, retro_mmus_483,
Pan troglodytes	ENSPTRG00000018347	1 retrocopy	retro_ptro_71,
Ictidomys tridecemlineatus	ENSSTOG00000007687	4 retrocopies	retro_itri_1509, retro_itri_1740, retro_itri_1816, retro_itri_195,
Drosophila melanogaster	FBgn0000557	1 retrocopy	retro_dmel_9,