RetrogeneDB ID: retro_pabe_2375

Retrocopy location

• Organism: Orangutan (Pongo abelii)
• Coordinates: 3:159967731..159968821(-)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: PA2G4
• Ensembl ID: ENSPPYG00000025851
• Aliases: None
• Description: proliferation-associated protein 2G4 [Source:RefSeq peptide;Acc:NP_001124717]

Sequence

>retro_pabe_2375
ATGTTGGGTAAGGATGAGCAGCAGGAGCAAACTATCGCTGAGGACCTGGTCGTGACCAAGTATAAGATGGAGGGCGACAT
CGCCAACAGGGGTACTTCGGTCCTTGGTGGAAGCATCTAGCTCAGGTGTGTCAGTACTGAGCCTGTGTGAGAAAGGTGAT
GCCATGATTATGGAAGAAACAGGGAAAATCTTCAAGAAAGAAAAGGAAATGAAGAAAGGTATTGCTTTTCCCACCAGCAT
TTCAGTAAATAACTGTGTATGTCACTTCTCCCCTTTGAAGAGCGACCAGGATTATATTCTCAAGGAAGGTGACTTGGTAA
AAATTGAACTTGGGGTCCATGTGGATGGCTTCATCGCTAATGTAGCTCATACTTTTGTGGTTGATGTAGCTCAGGGGACC
CAAGTAACAGGGAGGAAGGCAGATGTTATTAAGGCAGCCCACCTTTGTGTTGAAGCTACCCTACGCCTGGTCAAACCTGG
AAATCAGAACACACAAGTGACACAAGCCTGGAACAAAGTTGCCCACTCATTTAACTGCACACCAATAGATGGTATGCTGT
CACACCAGTTGAAGCAGCATGTCATTGATGGAGAAAAAACCATTATCCAGAATCCCACAGACCAGCAGAAGAAGGACCAT
GAAAAAGCTGAATTTGACATACATGAAGTGTATGCTGTGGATGTTCTCGTCAGCTCAGAAGAGGGCAAGGCCAAGGATGC
AGGACAGAGAACCACTATTTACAAATAAGACCCCTCTAAACAGTATGGACTGAAAATGAAAACTTCACGTGCCTTCTTCA
GTGAGGTGGAAAGGCGTTTTGATGCCATGCCGTTTACTTTAAGAGCATTTGAAGATGAGAAGAAGGCTCAGATGGGTGTG
GTGGAGTGCGCCAAACATGAACTGCTGCAACCATTTAATGTTCTCTATGAGAAGGAGGGTGAATTTGTTGCCCAGTTTAA
ATTTACAGTTCTGCTCATGCCCAGTGGCCCCATGCGGATAACCAGTGGTCCCTTCGAGCCTGACCTCTACAAGTCTGAGA
TGGAGGTCCAGGATGCAGAGCTAAAGGCCCTCCCCCAGAGTTCTACAAGT

Alignment

ORF - retro_pabe_2375 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 95.33 %
• Parental protein coverage: 92.13 %
• Number of stop codons detected: 1
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  MSGEDEQQEQTIAEDLVVTKYKMGGDIANR-VLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKE
          M.G.DEQQEQTIAEDLVVTKYKM.GDIANR.VLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKE
Retrocopy MLGKDEQQEQTIAEDLVVTKYKMEGDIANR>VLRSLVEASSSGVSVLSLCEKGDAMIMEETGKIFKKEKE

Parental  MKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIDLGVHVDGFIANVAHTFVVDVAQGTQVTGRK
          MKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKI.LGVHVDGFIANVAHTFVVDVAQGTQVTGRK
Retrocopy MKKGIAFPTSISVNNCVCHFSPLKSDQDYILKEGDLVKIELGVHVDGFIANVAHTFVVDVAQGTQVTGRK

Parental  ADVIKAAHLCAEAALRLVKPGNQNTQVTEAWNKVAHSFNCTPIEGMLSHQLKQHVIDGEKTIIQNPTDQQ
          ADVIKAAHLC.EA.LRLVKPGNQNTQVT.AWNKVAHSFNCTPI.GMLSHQLKQHVIDGEKTIIQNPTDQQ
Retrocopy ADVIKAAHLCVEATLRLVKPGNQNTQVTQAWNKVAHSFNCTPIDGMLSHQLKQHVIDGEKTIIQNPTDQQ

Parental  KKDHEKAEFEVHEVYAVDVLVSSGEGKAKDAGQRTTIYKRDPSKQYGLKMKTSRAFFSEVERRFDAMPFT
          KKDHEKAEF..HEVYAVDVLVSS.EGKAKDAGQRTTIYK.DPSKQYGLKMKTSRAFFSEVERRFDAMPFT
Retrocopy KKDHEKAEFDIHEVYAVDVLVSSEEGKAKDAGQRTTIYK*DPSKQYGLKMKTSRAFFSEVERRFDAMPFT

Parental  LRAFEDEKKARMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPNGPMRITSGPFEPDLYKSEMEV
          LRAFEDEKKA.MGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMP.GPMRITSGPFEPDLYKSEMEV
Retrocopy LRAFEDEKKAQMGVVECAKHELLQPFNVLYEKEGEFVAQFKFTVLLMPSGPMRITSGPFEPDLYKSEMEV

Parental  QDAELKALLQSSAS
          QDAELKAL.QSS.S
Retrocopy QDAELKALPQSSTS

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP007412_brain_prefrontal_cortex	0.06 RPM	51.81 RPM
SRP007412_cerebellum	0.85 RPM	46.69 RPM
SRP007412_heart	0.24 RPM	25.62 RPM
SRP007412_kidney	0.27 RPM	40.41 RPM
SRP007412_liver	0.28 RPM	34.00 RPM

ChIP-Seq

Pongo abelii was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_pabe_2375 retrocopy.

TSS

Pongo abelii was not studied using FANTOM5 data.

Lab

retro_pabe_2375 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_pabe_2375 has 1 orthologous retrocopies within eutheria group .

Species	RetrogeneDB ID
Homo sapiens	retro_hsap_2868

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Parental genes homology:

Parental genes homology involve 8 parental genes, and 27 retrocopies.

Species	Parental gene accession	Retrocopies number
Choloepus hoffmanni	ENSCHOG00000007181	2 retrocopies	retro_chof_1089, retro_chof_1847,
Homo sapiens	ENSG00000170515	6 retrocopies	retro_hsap_1930, retro_hsap_2413, retro_hsap_2868, retro_hsap_3646, retro_hsap_4191, retro_hsap_4895,
Macaca mulatta	ENSMMUG00000002164	2 retrocopies	retro_mmul_1168, retro_mmul_2631,
Nomascus leucogenys	ENSNLEG00000017544	6 retrocopies	retro_nleu_1212, retro_nleu_1260, retro_nleu_163, retro_nleu_3024, retro_nleu_506, retro_nleu_612,
Pongo abelii	ENSPPYG00000025851	7 retrocopies	retro_pabe_1595, retro_pabe_1778, retro_pabe_2375 , retro_pabe_2869, retro_pabe_2993, retro_pabe_3430, retro_pabe_3807,
Sus scrofa	ENSSSCG00000000377	1 retrocopy	retro_sscr_338,
Sus scrofa	ENSSSCG00000022666	2 retrocopies	retro_sscr_1143, retro_sscr_337,
Vicugna pacos	ENSVPAG00000009523	1 retrocopy	retro_vpac_2,