RetrogeneDB ID: retro_rnor_675

Retrocopy location

• Organism: Rat (Rattus norvegicus)
• Coordinates: 10:25954111..25954595(+)
• Located in intron of: None

Retrocopy information

• Ensembl ID: None
• Aliases: None
• Status: NOVEL

Parental gene information

• Parental gene symbol: Ndufaf4
• Ensembl ID: ENSRNOG00000007506
• Aliases: Ndufaf4, Hrpap20
• Description: NADH dehydrogenase [ubiquinone] 1 alpha subcomplex assembly factor 4 [Source:UniProtKB/Swiss-Prot;Acc:Q9NQR8]

Sequence

>retro_rnor_675
GTGAGCAGAGTAGGAGATCAACAAGAGGAAGTCCTTCATGGCACCCAAACACCCTTCCACCCTTCTACCAGCAGTCTCTT
GCAGAGGTACCTGAGTCAGTATCCAGAAATCTGAGGAAGAAGTTTCTAGGAAAGGTAAGAAGCTGCTGTCATGGCTAAGA
GATGTATATGTTGATTCTGAAGATCCGGTGCTTTTCTTGCCAGTAAAAGTTGTTGAACTACAGCAAGAACCAAAGGAATT
CAGACTGCCAATAGGAGATCACTTTGACAAGAATACTATGGACATTCCCAAAGGCAAGATTATTGTTGAAGGAGCATTGA
CCCTTCTCAATAACCATAAACTATCTCCAGAAACATGGACTGCTGAGAAAATCACCCAAGAATACCATTTAGAACTGAAG
GATGTAAATTCTCTATTCAAATATTTTGTTACTTTTGAAGTCAAAATCTTTCCTCCTGAAGACAGAAGAGCAATACAATC
AAAA

Alignment

ORF - retro_rnor_675 Open Reading Frame is not conserved.

Retrocopy - Parental Gene Alignment summary:

• Percent Identity: 74.07 %
• Parental protein coverage: 92.53 %
• Number of stop codons detected: 0
• Number of frameshifts detected: 1

Retrocopy - Parental Gene Alignment:

Parental  VEKRAEQEISKRKPSMAPKHPSTRSLLQEHLSQYPEI-EEEVSRKDNKLLSLLRDVYVNSKDPVPSLPVK
          V.....Q..........P.HPST.SLLQ..LSQYPEI.EEEVSRK..KLLS.LRDVYV.S.DPV..LPVK
Retrocopy VSRVGDQQEEVLHGTQTPFHPSTSSLLQRYLSQYPEI>EEEVSRKGKKLLSWLRDVYVDSEDPVLFLPVK

Parental  AIEPQQQPKEFRLPIGDQFDKNIKDIPKGKITVVEALTLLNNHKLSPETWTAEKIAQEYHLELEDVNSLL
          ..E.QQ.PKEFRLPIGD.FDKN..DIPKGKI.V..ALTLLNNHKLSPETWTAEKI.QEYHLEL.DVNSL.
Retrocopy VVELQQEPKEFRLPIGDHFDKNTMDIPKGKIIVEGALTLLNNHKLSPETWTAEKITQEYHLELKDVNSLF

Parental  KYFVTFEVKIVPPEDRKAIQSK
          KYFVTFEVKI.PPEDR.AIQSK
Retrocopy KYFVTFEVKIFPPEDRRAIQSK

Legend:

• *: Stop codon
• >: Forward frameshift by one nucleotide
• <: Reverse frameshift by one nucleotide

Expression

RNA-Seq

Expression validation based on RNA-Seq data:

Library	Retrocopy expression	Parental gene expression
SRP017611_brain	0.00 RPM	26.67 RPM
SRP017611_kidney	0.00 RPM	36.01 RPM
SRP017611_liver	0.00 RPM	12.10 RPM

ChIP-Seq

Rattus norvegicus was not studied using ChIP-Seq data.

EST

No EST(s) were mapped for retro_rnor_675 retrocopy.

TSS

Rattus norvegicus was not studied using FANTOM5 data.

Lab

retro_rnor_675 was not experimentally validated.

Homology

Retrocopy orthology:

Retrocopy retro_rnor_675 has 0 orthologous retrocopies within eutheria group .

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Parental genes homology:

Parental genes homology involve 15 parental genes, and 39 retrocopies.

Species	Parental gene accession	Retrocopies number
Bos taurus	ENSBTAG00000003174	1 retrocopy	retro_btau_1763,
Choloepus hoffmanni	ENSCHOG00000005145	2 retrocopies	retro_chof_2271, retro_chof_891,
Callithrix jacchus	ENSCJAG00000001235	2 retrocopies	retro_cjac_1613, retro_cjac_1700,
Cavia porcellus	ENSCPOG00000007618	1 retrocopy	retro_cpor_425,
Homo sapiens	ENSG00000123545	4 retrocopies	retro_hsap_1494, retro_hsap_2774, retro_hsap_3761, retro_hsap_477,
Gorilla gorilla	ENSGGOG00000012070	4 retrocopies	retro_ggor_1147, retro_ggor_1931, retro_ggor_2529, retro_ggor_451,
Microcebus murinus	ENSMICG00000015734	1 retrocopy	retro_mmur_1624,
Macaca mulatta	ENSMMUG00000018843	4 retrocopies	retro_mmul_1717, retro_mmul_2138, retro_mmul_578, retro_mmul_734,
Mus musculus	ENSMUSG00000028261	3 retrocopies	retro_mmus_1153, retro_mmus_1290, retro_mmus_3668,
Nomascus leucogenys	ENSNLEG00000013568	4 retrocopies	retro_nleu_171, retro_nleu_1716, retro_nleu_678, retro_nleu_890,
Oryctolagus cuniculus	ENSOCUG00000002152	1 retrocopy	retro_ocun_1162,
Pongo abelii	ENSPPYG00000016857	4 retrocopies	retro_pabe_1244, retro_pabe_2241, retro_pabe_257, retro_pabe_3160,
Pan troglodytes	ENSPTRG00000018435	4 retrocopies	retro_ptro_1013, retro_ptro_1877, retro_ptro_2552, retro_ptro_358,
Rattus norvegicus	ENSRNOG00000007506	3 retrocopies	retro_rnor_1617, retro_rnor_2973, retro_rnor_675 ,
Tursiops truncatus	ENSTTRG00000010911	1 retrocopy	retro_ttru_628,