| EST name | EST sequence |
| gi|30165495|gb|CB929224.1|CB929224 | GACGGCAGCGCAGGTCCACGCGGGCGCAGCGGAGGCAACGCGATGGTGGCTCGCCGCTTCGTGGTCCGGCATGGCCCCGC CGCCGCAGCCGGTGGCTCCGTCGAGCGGGAGGAGGAGGAGGAGCACGAGGTGGAGTACGATACGGAGCACGGCCTCGACG TCCTCCGCCTCCAGATCTTCTCCCTCACCGCCGTCCCGCCCGACCTCCAGAAGATCGTGGTGGAGGCGGATGGGTTGGTG GTGGACGACGGCACCGATCTGGAAACCGTCTCTGAGCGCCTGCGCCTCCTCGCTATCGGCGAGGAGGAGGAAGACGCCGC CGCAGCGGCCAGGGCGCAGGAAAAGTCCGATGAGGAATTCGCACGGATGCTACAGGCGGAGGAAGAGGCACTTCTCCTAC AACAGTACAGTATACAAAATGATGGTGGAGATGTGTTTAGACAGAGAGTTGAGCCTTACATGCACCATGTTCTGATGTAT GAAGATCCAGTGGGACAAGAGGCAGCACGTAAGACAGTTCCCATATGCGAACTGGAGGA |
Most similar to sp|Q7F0R1|PNG1_ORYSJ
>BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|Q10RE5|ARD2_ORYSJ 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 2 OS=Oryza sativa subsp. japonica GN=ARD2
PE=2 SV=1
Length = 198
Score = 294 bits (753), Expect(3) = 6e-95
Identities = 135/141 (95%), Positives = 140/141 (99%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
ME+QFQDGKEEVI+AWYMDDSEEDQRLPHHREPKEFIPL KLSELGILSWRLNADDWEND
Sbjct: 1 MENQFQDGKEEVIEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWEND 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
ENLKKIREARGYSYMDICDVCPEKLPNYEAK+KNFFEEHLHTDEEIRYCLEGSGYFDVRD
Sbjct: 61 ENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
QND+WIR+AVKKGGMIVLPAG
Sbjct: 121 QNDQWIRVAVKKGGMIVLPAG 141
Score = 60.5 bits (145), Expect(3) = 6e-95
Identities = 24/27 (88%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVGEPVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGEPVWTPYNRPHDHLPARK 180
Score = 36.2 bits (82), Expect(3) = 6e-95
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1
Query: 625 EYVERIINRGGSQAVEAR 678
EYVE+IINRGG+QAVEAR
Sbjct: 181 EYVEKIINRGGTQAVEAR 198
>sp|A2XCT8|ARD2_ORYSI 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 2 OS=Oryza sativa subsp. indica GN=ARD2 PE=2
SV=2
Length = 198
Score = 294 bits (753), Expect(3) = 6e-95
Identities = 135/141 (95%), Positives = 140/141 (99%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
ME+QFQDGKEEVI+AWYMDDSEEDQRLPHHREPKEFIPL KLSELGILSWRLNADDWEND
Sbjct: 1 MENQFQDGKEEVIEAWYMDDSEEDQRLPHHREPKEFIPLSKLSELGILSWRLNADDWEND 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
ENLKKIREARGYSYMDICDVCPEKLPNYEAK+KNFFEEHLHTDEEIRYCLEGSGYFDVRD
Sbjct: 61 ENLKKIREARGYSYMDICDVCPEKLPNYEAKLKNFFEEHLHTDEEIRYCLEGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
QND+WIR+AVKKGGMIVLPAG
Sbjct: 121 QNDQWIRVAVKKGGMIVLPAG 141
Score = 60.5 bits (145), Expect(3) = 6e-95
Identities = 24/27 (88%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVGEPVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGEPVWTPYNRPHDHLPARK 180
Score = 36.2 bits (82), Expect(3) = 6e-95
Identities = 16/18 (88%), Positives = 18/18 (100%)
Frame = +1
Query: 625 EYVERIINRGGSQAVEAR 678
EYVE+IINRGG+QAVEAR
Sbjct: 181 EYVEKIINRGGTQAVEAR 198
>sp|A1L4T4|ARD1_ORYSJ 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 1 OS=Oryza sativa subsp. japonica GN=ARD1
PE=2 SV=1
Length = 199
Score = 273 bits (697), Expect(2) = 9e-84
Identities = 123/141 (87%), Positives = 136/141 (96%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
ME++FQDGK EVI+AWYMDDSEEDQRLPHHREPKEFI +DKL+ELG++SWRLN D+WEN
Sbjct: 1 MENEFQDGKTEVIEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENC 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
ENLK+IREARGYSY+DICDVCPEKLPNYE KIK+FFEEHLHTDEEIRYCLEGSGYFDVRD
Sbjct: 61 ENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
QND+WIRIA+KKGGMIVLPAG
Sbjct: 121 QNDQWIRIALKKGGMIVLPAG 141
Score = 59.3 bits (142), Expect(2) = 9e-84
Identities = 23/27 (85%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVG+PVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGDPVWTPYNRPHDHLPARK 180
>sp|A2Z7C4|ARD1_ORYSI 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 1 OS=Oryza sativa subsp. indica GN=ARD1 PE=1
SV=2
Length = 199
Score = 273 bits (697), Expect(2) = 9e-84
Identities = 123/141 (87%), Positives = 136/141 (96%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
ME++FQDGK EVI+AWYMDDSEEDQRLPHHREPKEFI +DKL+ELG++SWRLN D+WEN
Sbjct: 1 MENEFQDGKTEVIEAWYMDDSEEDQRLPHHREPKEFIHVDKLTELGVISWRLNPDNWENC 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
ENLK+IREARGYSY+DICDVCPEKLPNYE KIK+FFEEHLHTDEEIRYCLEGSGYFDVRD
Sbjct: 61 ENLKRIREARGYSYVDICDVCPEKLPNYETKIKSFFEEHLHTDEEIRYCLEGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
QND+WIRIA+KKGGMIVLPAG
Sbjct: 121 QNDQWIRIALKKGGMIVLPAG 141
Score = 59.3 bits (142), Expect(2) = 9e-84
Identities = 23/27 (85%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVG+PVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGDPVWTPYNRPHDHLPARK 180
>sp|Q8GXE2|ARD2_ARATH 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 2 OS=Arabidopsis thaliana GN=ARD2 PE=2 SV=1
Length = 192
Score = 251 bits (641), Expect(2) = 1e-77
Identities = 110/141 (78%), Positives = 131/141 (92%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
M + +DG+EEVIQAWYMDDSEEDQRLPHH++PKEF+ LDKL+ELG+LSWRL+AD++E D
Sbjct: 1 MGEAVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFVSLDKLAELGVLSWRLDADNYETD 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
E+LKKIRE+RGYSYMD C+VCPEKLPNYE K+K+FFEEHLHTDEEIRYC+ G+GYFDVRD
Sbjct: 61 EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGTGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
+N+ WIR+ VKKGGMIVLPAG
Sbjct: 121 RNEAWIRVLVKKGGMIVLPAG 141
Score = 60.5 bits (145), Expect(2) = 1e-77
Identities = 24/27 (88%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVGEPVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGEPVWTPYNRPHDHLPARK 180
>sp|Q8W108|ARD3_ARATH 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 3 OS=Arabidopsis thaliana GN=ARD3 PE=2 SV=1
Length = 199
Score = 251 bits (640), Expect(2) = 2e-77
Identities = 111/141 (78%), Positives = 131/141 (92%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
M + +DG+EEVIQAWYMDDSEEDQRLPHH++PKEF+ LDKL+ELG+LSWRL+AD++E D
Sbjct: 1 MGEVVKDGREEVIQAWYMDDSEEDQRLPHHKDPKEFLSLDKLAELGVLSWRLDADNYETD 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
E+LKKIRE+RGYSYMD C+VCPEKLPNYE K+K+FFEEHLHTDEEIRYC+ GSGYFDVRD
Sbjct: 61 EDLKKIRESRGYSYMDFCEVCPEKLPNYEVKVKSFFEEHLHTDEEIRYCVAGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
+N+ WIR+ VKKGGMIVLPAG
Sbjct: 121 RNEAWIRVWVKKGGMIVLPAG 141
Score = 60.5 bits (145), Expect(2) = 2e-77
Identities = 24/27 (88%), Positives = 27/27 (100%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVGEPVWTPYNRPHDHLPAR+
Sbjct: 154 IKAMRLFVGEPVWTPYNRPHDHLPARK 180
>sp|O48707|ARD1_ARATH 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 1 OS=Arabidopsis thaliana GN=ARD1 PE=2 SV=1
Length = 199
Score = 233 bits (593), Expect(2) = 2e-70
Identities = 103/141 (73%), Positives = 123/141 (87%)
Frame = +2
Query: 128 MEDQFQDGKEEVIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWEND 307
M + +D EEVIQAWY+D+ EEDQ+LPHH++PKEF+ LDKL+ELG+L WRL+AD++E D
Sbjct: 1 MGEAAKDQTEEVIQAWYLDNKEEDQKLPHHKDPKEFVSLDKLAELGVLCWRLDADNYETD 60
Query: 308 ENLKKIREARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRD 487
E LK+IRE+RGYSYMD+C+VCPEKLPNYE K+K FFEEHLH DEEIRYCL GSGYFDVRD
Sbjct: 61 EELKRIRESRGYSYMDLCEVCPEKLPNYEEKVKMFFEEHLHIDEEIRYCLAGSGYFDVRD 120
Query: 488 QNDKWIRIAVKKGGMIVLPAG 550
ND WIR+ VKKGG+IV PAG
Sbjct: 121 LNDIWIRVWVKKGGLIVFPAG 141
Score = 55.1 bits (131), Expect(2) = 2e-70
Identities = 22/32 (68%), Positives = 27/32 (84%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARRGVCRE 638
++AMRLFVG PVWT YNRPHDHLPAR+ ++
Sbjct: 154 MKAMRLFVGGPVWTAYNRPHDHLPARKAYMKK 185
>sp|Q7XNS7|ARD3_ORYSJ Putative
1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase 3
OS=Oryza sativa subsp. japonica GN=ARD3 PE=3 SV=3
Length = 206
Score = 202 bits (515), Expect(2) = 6e-59
Identities = 90/135 (66%), Positives = 116/135 (85%), Gaps = 3/135 (2%)
Frame = +2
Query: 155 EEVIQAWYMDD---SEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKI 325
EEV+QAWYMDD +EEDQRLPH R+P + +PL KL +LG+++ RL+AD+ E+DENLK +
Sbjct: 9 EEVVQAWYMDDDDNAEEDQRLPHRRQPDDLLPLAKLLDLGLVAMRLDADNHEHDENLKIM 68
Query: 326 REARGYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWI 505
RE RGY +MDI ++ PEK+PNYE IK FFEEHLHTDEE+RYCL+GSGYFDVRD+NDKW+
Sbjct: 69 REQRGYLHMDIVELTPEKMPNYEVMIKRFFEEHLHTDEEVRYCLDGSGYFDVRDENDKWV 128
Query: 506 RIAVKKGGMIVLPAG 550
R++V+KG +IV+PAG
Sbjct: 129 RVSVRKGALIVVPAG 143
Score = 46.6 bits (109), Expect(2) = 6e-59
Identities = 21/40 (52%), Positives = 25/40 (62%), Gaps = 2/40 (5%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARRGVCR--ENHQPRW 656
++ MRLF G P WT YNRPHDHLP R+ N PR+
Sbjct: 156 IKTMRLFSGGPDWTAYNRPHDHLPERKKYLEALHNRTPRF 195
>sp|Q8H185|ARD4_ARATH 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 4 OS=Arabidopsis thaliana GN=ARD4 PE=2 SV=1
Length = 187
Score = 202 bits (513), Expect(2) = 1e-58
Identities = 87/129 (67%), Positives = 108/129 (83%)
Frame = +2
Query: 164 IQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARGY 343
++AW+MDDS EDQRLPHHR PKE + LD L+ELG+L W+LN +++END L KIRE RGY
Sbjct: 3 LEAWFMDDSNEDQRLPHHRNPKELVSLDYLAELGVLYWKLNPENYENDSELSKIREDRGY 62
Query: 344 SYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVKK 523
YMD+ D+CPEK+ NYE K+KNFF EH+H DEEIRYCL GSGYFDVRD++D+WIRI ++
Sbjct: 63 DYMDLLDLCPEKVSNYEEKLKNFFTEHIHKDEEIRYCLAGSGYFDVRDKDDRWIRIWMQP 122
Query: 524 GGMIVLPAG 550
G +IVLPAG
Sbjct: 123 GDLIVLPAG 131
Score = 46.6 bits (109), Expect(2) = 1e-58
Identities = 18/27 (66%), Positives = 22/27 (81%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++ MRLFVGEPVWTPYNRP + P R+
Sbjct: 144 IKLMRLFVGEPVWTPYNRPQEEHPVRK 170
>sp|Q562C9|MTND_RAT 1,2-dihydroxy-3-keto-5-methylthiopentene
Length = 179
Score = 180 bits (456), Expect(2) = 2e-53
Identities = 80/130 (61%), Positives = 102/130 (78%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
++QAWYMD+S D R+PH +P + L++L LG+L W+L+AD +END L++IR+ R
Sbjct: 1 MVQAWYMDESTADPRMPHRAQPDRPVGLEQLRTLGVLYWKLDADKYENDPELEQIRKTRN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
YS+MDI +C + LPNYE KIK FFEEHLH DEEIRY LEGSGYFDVRD+ DKWIRI+++
Sbjct: 61 YSWMDIITICKDSLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISME 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 50.8 bits (120), Expect(2) = 2e-53
Identities = 21/26 (80%), Positives = 23/26 (88%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPAR 620
++AMRLFVGEPVWTPYNRP DH AR
Sbjct: 143 VKAMRLFVGEPVWTPYNRPADHFDAR 168
>sp|Q99JT9|MTND_MOUSE 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Mus musculus GN=Adi1 PE=1 SV=1
Length = 179
Score = 179 bits (455), Expect(2) = 3e-53
Identities = 81/130 (62%), Positives = 101/130 (77%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
++QAWYMD+S D R PH +P + L++L LG+L W+L+AD +END L+KIR+ R
Sbjct: 1 MVQAWYMDESTADPRKPHRAQPDRPVSLEQLRTLGVLYWKLDADKYENDPELEKIRKMRN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
YS+MDI +C + LPNYE KIK FFEEHLH DEEIRY LEGSGYFDVRD+ DKWIRI+++
Sbjct: 61 YSWMDIITICKDTLPNYEEKIKMFFEEHLHLDEEIRYILEGSGYFDVRDKEDKWIRISME 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 50.8 bits (120), Expect(2) = 3e-53
Identities = 21/26 (80%), Positives = 23/26 (88%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPAR 620
++AMRLFVGEPVWTPYNRP DH AR
Sbjct: 143 VKAMRLFVGEPVWTPYNRPADHFDAR 168
>sp|Q3ZBL1|MTND_BOVIN 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Bos taurus GN=ADI1 PE=2 SV=1
Length = 179
Score = 178 bits (451), Expect(2) = 4e-53
Identities = 79/130 (60%), Positives = 103/130 (79%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
+++AWY+DD+ +D RLPH EP + L++L LG+L W+L+AD +END L+KIR+ R
Sbjct: 1 MVEAWYIDDAADDPRLPHRAEPARPVGLEQLRRLGVLYWKLDADKYENDPELEKIRKERN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
YS++DI + +KLPNYE KIK FFEEHLH DEEIRY L+GSGYFDVRDQ D+WIRI+++
Sbjct: 61 YSWVDIITISKDKLPNYEEKIKMFFEEHLHLDEEIRYILDGSGYFDVRDQEDRWIRISME 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 51.6 bits (122), Expect(2) = 4e-53
Identities = 21/26 (80%), Positives = 24/26 (92%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPAR 620
++AMRLFVG+PVWTPYNRP DHL AR
Sbjct: 143 VKAMRLFVGDPVWTPYNRPSDHLEAR 168
>sp|Q5ZL43|MTND_CHICK 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Gallus gallus GN=ADI1 PE=2 SV=1
Length = 180
Score = 175 bits (443), Expect(2) = 4e-51
Identities = 78/130 (60%), Positives = 103/130 (79%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
+++AWYMD+S+EDQR PH +P + L++L LG+ RL+AD++E D LK+IREA
Sbjct: 1 MVEAWYMDESQEDQRAPHRLQPNRAVSLEQLRRLGVAYRRLDADNYETDPRLKEIREAEN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
YS+MDI + +KLPNYE KIK F+EEHLH D+EIRY L+GSGYFDVRD++DKWIRI+++
Sbjct: 61 YSWMDIVTIHKDKLPNYEEKIKTFYEEHLHLDDEIRYILDGSGYFDVRDKDDKWIRISME 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 48.1 bits (113), Expect(2) = 4e-51
Identities = 20/27 (74%), Positives = 23/27 (85%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++AMRLFVGEPVWT YNRP D PAR+
Sbjct: 143 VKAMRLFVGEPVWTAYNRPADDFPARK 169
>sp|Q9BV57|MTND_HUMAN 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Homo sapiens GN=ADI1 PE=1 SV=1
Length = 179
Score = 174 bits (441), Expect(2) = 1e-50
Identities = 77/130 (59%), Positives = 100/130 (76%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
++QAWYMDD+ D R PH +P + L++L LG+L W+L+AD +END L+KIR R
Sbjct: 1 MVQAWYMDDAPGDPRQPHRPDPGRPVGLEQLRRLGVLYWKLDADKYENDPELEKIRRERN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
YS+MDI +C +KLPNYE KIK F+EEHLH D+EIRY L+GSGYFDVRD+ D+WIRI ++
Sbjct: 61 YSWMDIITICKDKLPNYEEKIKMFYEEHLHLDDEIRYILDGSGYFDVRDKEDQWIRIFME 120
Query: 521 KGGMIVLPAG 550
KG M+ LPAG
Sbjct: 121 KGDMVTLPAG 130
Score = 47.4 bits (111), Expect(2) = 1e-50
Identities = 20/25 (80%), Positives = 21/25 (84%)
Frame = +3
Query: 546 QAMRLFVGEPVWTPYNRPHDHLPAR 620
+AMRLFVGEPVWT YNRP DH AR
Sbjct: 144 KAMRLFVGEPVWTAYNRPADHFEAR 168
>sp|Q6DIY2|MTND_XENTR 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Xenopus tropicalis GN=adi1 PE=2 SV=1
Length = 179
Score = 167 bits (424), Expect(2) = 1e-48
Identities = 75/130 (57%), Positives = 101/130 (77%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
++QAWYMDDS EDQR PHH +P++ + L++L +G+ S+ L+AD +E+D L KIR+
Sbjct: 1 MVQAWYMDDSTEDQRKPHHLQPEQPVSLEQLKAVGVHSFSLDADRYESDPELAKIRQENN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
Y++MDI + + LPNYE K+K F+EEHLH D+EIRY LEGSGYFDVRD+ D+WIRI ++
Sbjct: 61 YTWMDIITIHKDTLPNYEEKLKIFYEEHLHLDDEIRYILEGSGYFDVRDKEDRWIRIFMQ 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 47.0 bits (110), Expect(2) = 1e-48
Identities = 19/26 (73%), Positives = 23/26 (88%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPAR 620
++AMRLFVG+PVWTPYNRP D+ AR
Sbjct: 143 VKAMRLFVGDPVWTPYNRPADNFEAR 168
>sp|Q6PBX5|MTND_DANRE 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Danio rerio GN=adi1 PE=2 SV=1
Length = 181
Score = 173 bits (438), Expect(2) = 3e-48
Identities = 78/131 (59%), Positives = 101/131 (77%), Gaps = 1/131 (0%)
Frame = +2
Query: 161 VIQAWYMDD-SEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREAR 337
V +AWYMD+ S EDQRLPH P + + + +L +G+ W+LNAD +END L+KIRE +
Sbjct: 3 VFEAWYMDEESGEDQRLPHKLSPNQPVSVQQLEHIGVFHWKLNADIYENDPELQKIREEK 62
Query: 338 GYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAV 517
GYS+MDI + P+KLP+Y+ K+K F+EEHLH D+EIRY LEGS YFDVRD+ D+WIRIAV
Sbjct: 63 GYSFMDIITIHPDKLPDYQNKLKMFYEEHLHLDDEIRYILEGSSYFDVRDEGDRWIRIAV 122
Query: 518 KKGGMIVLPAG 550
KG +I LPAG
Sbjct: 123 SKGDLITLPAG 133
Score = 40.4 bits (93), Expect(2) = 3e-48
Identities = 17/26 (65%), Positives = 19/26 (73%)
Frame = +3
Query: 546 QAMRLFVGEPVWTPYNRPHDHLPARR 623
+AMRLFVGEPVW YNRP D R+
Sbjct: 147 KAMRLFVGEPVWKAYNRPADDFDIRK 172
>sp|Q3B8C8|MTND_XENLA 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Xenopus laevis GN=adi1 PE=2 SV=1
Length = 179
Score = 160 bits (405), Expect(2) = 8e-46
Identities = 75/130 (57%), Positives = 96/130 (73%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARG 340
++QAWYMDDS EDQR PH + + L++L LG+ S L+AD +E+D L KIR+
Sbjct: 1 MVQAWYMDDSAEDQRKPHRLQQDVPVSLEQLKALGVDSLSLDADRYESDPELAKIRKENN 60
Query: 341 YSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVK 520
Y++MDI + + +PNYE K+K F+EEHLH D+EIRY LEGSGYFDVRDQ DKWIRI ++
Sbjct: 61 YTWMDIITIHKDTMPNYEEKLKIFYEEHLHLDDEIRYILEGSGYFDVRDQKDKWIRIFMQ 120
Query: 521 KGGMIVLPAG 550
KG MI LPAG
Sbjct: 121 KGDMITLPAG 130
Score = 45.1 bits (105), Expect(2) = 8e-46
Identities = 18/26 (69%), Positives = 23/26 (88%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPAR 620
++AMRLFVG+PVWTP+NRP D+ AR
Sbjct: 143 VKAMRLFVGDPVWTPFNRPADNCEAR 168
>sp|Q7XEJ5|ARD4_ORYSJ 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase 4 OS=Oryza sativa subsp. japonica GN=ARD4
PE=2 SV=1
Length = 184
Score = 158 bits (399), Expect(2) = 2e-44
Identities = 69/126 (54%), Positives = 95/126 (75%)
Frame = +2
Query: 173 WYMDDSEEDQRLPHHREPKEFIPLDKLSELGILSWRLNADDWENDENLKKIREARGYSYM 352
W + ++ E++ + P E +PL +L E+G+L W L+ E++E L KIR RGYSY
Sbjct: 6 WMLGENGEEKSFEN---PNELLPLSRLEEIGVLYWHLDPKKSESEEELTKIRRERGYSYF 62
Query: 353 DICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVKKGGM 532
D+ ++CP+KL NYE K+K+F+ EH+H DEEIRYCLEGSGYFDVRD++DKWIRI +K+G M
Sbjct: 63 DLTEICPDKLENYEEKLKSFYCEHIHADEEIRYCLEGSGYFDVRDKDDKWIRIWIKEGDM 122
Query: 533 IVLPAG 550
I+LPAG
Sbjct: 123 IILPAG 128
Score = 42.4 bits (98), Expect(2) = 2e-44
Identities = 16/27 (59%), Positives = 21/27 (77%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARR 623
++ MRLF+GEPVWT YNRP + P R+
Sbjct: 141 IKLMRLFIGEPVWTAYNRPQEDHPVRQ 167
>sp|Q6AWN0|MTND_DROME 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Drosophila melanogaster GN=CG32068 PE=1
SV=2
Length = 186
Score = 148 bits (373), Expect(2) = 3e-40
Identities = 68/131 (51%), Positives = 91/131 (69%), Gaps = 1/131 (0%)
Frame = +2
Query: 161 VIQAWYMDDSEEDQRLPHHREPKEFIPLDKL-SELGILSWRLNADDWENDENLKKIREAR 337
++Q WYMD E DQRL HHR P ++ LD L + G+ +++NAD++++D L ++R R
Sbjct: 1 MVQVWYMDTEETDQRLEHHRNPPAYLELDDLYQKTGVEYFKINADEYQSDNTLTELRAKR 60
Query: 338 GYSYMDICDVCPEKLPNYEAKIKNFFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAV 517
GY+Y D + LP+Y K+K FF EHLHTDEEIR LEGSGYFDVRD D W+RI V
Sbjct: 61 GYTYDDEITCSEKCLPDYANKLKAFFTEHLHTDEEIRLILEGSGYFDVRDNEDNWLRIKV 120
Query: 518 KKGGMIVLPAG 550
KG +I++PAG
Sbjct: 121 VKGDLIIIPAG 131
Score = 38.9 bits (89), Expect(2) = 3e-40
Identities = 14/35 (40%), Positives = 21/35 (60%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNRPHDHLPARRGVCRENHQ 647
++ R FVGEPVW P+NRP D + R+ + +
Sbjct: 144 IRTRRYFVGEPVWAPHNRPADEMDCRKSYIKHQSE 178
>sp|Q54ER6|MTND_DICDI 1,2-dihydroxy-3-keto-5-methylthiopentene
dioxygenase OS=Dictyostelium discoideum GN=adi1 PE=3
SV=1
Length = 147
Score = 95.1 bits (235), Expect(2) = 8e-21
Identities = 47/106 (44%), Positives = 68/106 (64%)
Frame = +2
Query: 230 EFIPLDKLSELGILSWRLNADDWENDENLKKIREARGYSYMDICDVCPEKLPNYEAKIKN 409
+ I LD L E +L + ++ + E L+ + + RGY D + E P E K+K
Sbjct: 11 DVISLDYLKEHNVLYFPMDREKENYKEPLETLCKERGYKNRDEVKLSKET-PGLEDKLKI 69
Query: 410 FFEEHLHTDEEIRYCLEGSGYFDVRDQNDKWIRIAVKKGGMIVLPA 547
FFEEHLH DEEIR+ L+G G+FD+RD+NDKW++I KKG +I++PA
Sbjct: 70 FFEEHLHDDEEIRFILDGCGFFDIRDKNDKWVKIKEKKGDLIIVPA 115
Score = 26.6 bits (57), Expect(2) = 8e-21
Identities = 10/18 (55%), Positives = 11/18 (61%)
Frame = +3
Query: 543 LQAMRLFVGEPVWTPYNR 596
+ AMRLF P W P NR
Sbjct: 129 IHAMRLFTDAPKWVPINR 146
BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|Q7F0R1|PNG1_ORYSJ
amidase OS=Oryza sativa subsp. japonica GN=PNG1 PE=2
SV=1
Length = 447
Score = 164 bits (414), Expect = 4e-40
Identities = 94/170 (55%), Positives = 105/170 (61%), Gaps = 5/170 (2%)
Frame = +1
Query: 43 MVARRFVVRHGPAAAAGGSVXXXXXXXXXXXXXXXHGLDVLRLQIFSLTAVPPDLQKIVV 222
MVARRFVVR G GG HGLD+LRLQIFSLT+VPP+LQKIVV
Sbjct: 1 MVARRFVVRQGGGGGGGGEAEEHEVEYDTE-----HGLDILRLQIFSLTSVPPELQKIVV 55
Query: 223 EADGLVVDDGTDLETVSERLRLLXXXXXXXXXXXXXXXXX-----KSDEEFARMXXXXXX 387
EADG VVDDGTDLE +SE LRL+ KSDEE ARM
Sbjct: 56 EADGSVVDDGTDLEAISEGLRLVAITGEEEEAEAAAAAEAARAQEKSDEELARMIQAEEE 115
Query: 388 XXXXXXYSIQNDGGDVFRQRVEPYMHHVLMYEDPVGQEAARKTVPICELE 537
YSI+NDGG+ FR+RVEPYMH VLMYEDP+ QEAARKTVP+ EL+
Sbjct: 116 ALLLQQYSIRNDGGEEFRERVEPYMHQVLMYEDPMRQEAARKTVPMDELQ 165