| EST name | EST sequence |
| gi|45986090|gb|CN146275.1|CN146275 | GCCATGGTCCCAGCCTACGACAAGACCAAGTACACCGGCATTGGGTCTGCCATTGAGTACGCCGTGTGCGCCCTCAAGGT GCAGGTCCTCGTCGTCATTGGCCACAGCTGCTGTGGTGGCATCAGGGCGCTCCTCTCCCTCCAGGATGGCGCACCTGACA CCTTCCATTTGGTTGAGGACTGGGTCAGGATCGGCTTCCCTGCCAAGATCAAGGTGAAGAAAGAGCACGCTTCGGTGCCG TTCGATGACCAGTGTTCCATCCTGGAGAAGGAGGCCGTGAACGTGTCCCTCGACAACCTCAAGACGTACCCCTTCGTCAA GGAAGGGCTGGCCAACGGGACCCTCAGGCTGGTCGGCGGCCACTACAACTTCGTGTCAGGGGAGTTCGACACATGGGAAA TTAAGAGCTAAGCTGCCCGGCCGGCCGCTTAACAACTCCTACCATCATCATAAATATATATACTACATACCTACCGATAT GCACCCGATCGATGTGAATGCGTGGAGTATTACTCATGTTTTCTGCATGCAGCGACATATATATATATATACCGGATCGC CCAATGTGAATGTAATAAGTAATAGCATTTTCTACCGCTTTTATTTCTAACGCCGAGCTTTCATGTACTATATAATGTGT TATATGATGAGTAATTTGACCGCCCTGTGGTCAAAAGACATCAGCTATAATCCTTTTTTCGCAATATTTATTATGAAGCG TTTACCTCTTGTACTGCCTTT |
Most similar to sp|P40880|CAHC_HORVU
>BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|Q6H849|NRL4_ORYSJ Bifunctional nitrilase/nitrile hydratase NIT4
OS=Oryza sativa subsp. japonica GN=NIT4 PE=2 SV=1
Length = 362
Score = 99.4 bits (246), Expect(2) = 2e-33
Identities = 49/57 (85%), Positives = 52/57 (91%)
Frame = +3
Query: 306 VAMNGGADPSATTVRATVVQASTIFHDTPATLDKAERLIAEAAGYGSQLVVFPEAFI 476
V MNGGAD A TVRATVVQAST+F+DTPATLDKAERLI EAAGYGSQLVVFPEAF+
Sbjct: 18 VEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAAGYGSQLVVFPEAFV 74
Score = 64.3 bits (155), Expect(2) = 2e-33
Identities = 29/45 (64%), Positives = 33/45 (73%)
Frame = +2
Query: 467 SFY*GYPRGSTFGFXXXXXXXNPKDRGKEAFRMYHASAIDVPGSE 601
+F GYPRGSTFGF NPKD+GKE FR YHA+AI+VPG E
Sbjct: 72 AFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPE 116
>sp|Q42966|NRL4B_TOBAC Bifunctional nitrilase/nitrile hydratase
NIT4B OS=Nicotiana tabacum GN=NIT4B PE=2 SV=1
Length = 348
Score = 85.5 bits (210), Expect(2) = 2e-25
Identities = 43/52 (82%), Positives = 47/52 (90%)
Frame = +3
Query: 321 GADPSATTVRATVVQASTIFHDTPATLDKAERLIAEAAGYGSQLVVFPEAFI 476
G + S TVRATVVQASTIF+DTPATLDKAERL+AEAA YG+QLVVFPEAFI
Sbjct: 21 GDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASYGAQLVVFPEAFI 72
Score = 51.2 bits (121), Expect(2) = 2e-25
Identities = 27/45 (60%), Positives = 29/45 (64%)
Frame = +2
Query: 467 SFY*GYPRGSTFGFXXXXXXXNPKDRGKEAFRMYHASAIDVPGSE 601
+F GYPRGSTFG N +GKE FR YHASAIDVPG E
Sbjct: 70 AFIGGYPRGSTFGVSIG----NRTAKGKEEFRKYHASAIDVPGPE 110
>sp|Q42965|NRL4A_TOBAC Bifunctional nitrilase/nitrile hydratase
NIT4A OS=Nicotiana tabacum GN=NIT4A PE=2 SV=1
Length = 349
Score = 82.0 bits (201), Expect(2) = 2e-24
Identities = 42/52 (80%), Positives = 46/52 (88%)
Frame = +3
Query: 321 GADPSATTVRATVVQASTIFHDTPATLDKAERLIAEAAGYGSQLVVFPEAFI 476
G + S TVRATVVQASTIF+DTPATL KAERL+AEAA YG+QLVVFPEAFI
Sbjct: 21 GDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASYGAQLVVFPEAFI 72
Score = 51.2 bits (121), Expect(2) = 2e-24
Identities = 27/45 (60%), Positives = 29/45 (64%)
Frame = +2
Query: 467 SFY*GYPRGSTFGFXXXXXXXNPKDRGKEAFRMYHASAIDVPGSE 601
+F GYPRGSTFG N +GKE FR YHASAIDVPG E
Sbjct: 70 AFIGGYPRGSTFGVSIG----NRTAKGKEEFRKYHASAIDVPGPE 110
>sp|P46011|NRL4_ARATH Bifunctional nitrilase/nitrile hydratase NIT4
OS=Arabidopsis thaliana GN=NIT4 PE=1 SV=1
Length = 355
Score = 79.0 bits (193), Expect(2) = 2e-21
Identities = 40/51 (78%), Positives = 46/51 (90%)
Frame = +3
Query: 324 ADPSATTVRATVVQASTIFHDTPATLDKAERLIAEAAGYGSQLVVFPEAFI 476
A S++ VRATVVQAST+F+DTPATLDKAERL++EAA GSQLVVFPEAFI
Sbjct: 29 AGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQLVVFPEAFI 79
Score = 43.9 bits (102), Expect(2) = 2e-21
Identities = 23/45 (51%), Positives = 27/45 (60%)
Frame = +2
Query: 467 SFY*GYPRGSTFGFXXXXXXXNPKDRGKEAFRMYHASAIDVPGSE 601
+F GYPRGSTF +G++ FR YHASAIDVPG E
Sbjct: 77 AFIGGYPRGSTFELAIGSRTA----KGRDDFRKYHASAIDVPGPE 117
BLASTX 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>sp|P40880|CAHC_HORVU Carbonic anhydrase, chloroplastic OS=Hordeum
vulgare PE=2 SV=1
Length = 324
Score = 223 bits (568), Expect = 1e-57
Identities = 105/132 (79%), Positives = 120/132 (90%)
Frame = +1
Query: 4 MVPAYDKTKYTGIGSAIEYAVCALKVQVLVVIGHSCCGGIRALLSLQDGAPDTFHLVEDW 183
MVPAY K KY G+GSAIEYAVCALKV+V+VVIGHS CGGI+ALLSL+DGA D+FH VEDW
Sbjct: 192 MVPAYCKNKYAGVGSAIEYAVCALKVEVIVVIGHSRCGGIKALLSLKDGADDSFHFVEDW 251
Query: 184 VRIGFPAKIKVKKEHASVPFDDQCSILEKEAVNVSLDNLKTYPFVKEGLANGTLRLVGGH 363
VRIGFPAK KV+ E AS+PFDDQC++LEKEAVNVSL NL TYPFVKEG+ NGTL+LVGGH
Sbjct: 252 VRIGFPAKKKVQTECASMPFDDQCTVLEKEAVNVSLQNLLTYPFVKEGVTNGTLKLVGGH 311
Query: 364 YNFVSGEFDTWE 399
Y+FVSG+F+TWE
Sbjct: 312 YDFVSGKFETWE 323