miRNEST 2.0: an integrative microRNA resource miRNEST 2.0, an integrative microRNA resource :: BLAST


>54628_sof-miR159a:pmrd
          Length = 265

 Score =  305 bits (154), Expect = 9e-84
 Identities = 238/263 (90%), Gaps = 6/263 (2%)
 Strand = Plus / Plus

                                                                       
Query: 1   ctgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggt 60
           |||||||||||||| ||||||||||||||||||||||||||||||||| || | | ||||
Sbjct: 2   ctgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggt 61

                                                                       
Query: 61  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtac 120
            ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| 
Sbjct: 62  tgttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtat 121

                                                                       
Query: 121 tctagag-ggccggagaagagat----tcatgtggtcgtcagtctttgagataggcttgt 175
           | | ||| ||  ||||| ||| |    ||   |||| ||| |||||||||||||||||||
Sbjct: 122 tttcgagagggaggagaggagctagactctcatggtggtc-gtctttgagataggcttgt 180

                                                                       
Query: 176 gatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggga 235
           | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 ggtttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggga 240

                                  
Query: 236 gctctgcatcctgatccatccat 258
           |||||||||||||||||||||||
Sbjct: 241 gctctgcatcctgatccatccat 263




>54629_sof-miR159b:pmrd
          Length = 266

 Score =  291 bits (147), Expect = 1e-79
 Identities = 238/264 (90%), Gaps = 7/264 (2%)
 Strand = Plus / Plus

                                                                       
Query: 1   ctgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggt 60
           |||||||||||||| ||||||||||||||||||||||||||||||||| || | | ||||
Sbjct: 2   ctgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggt 61

                                                                       
Query: 61  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtac 120
           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| 
Sbjct: 62  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtat 121

                                                                       
Query: 121 tct---agagggccg-gagaag--agattcatgtggtcgtcagtctttgagataggcttg 174
           | |   ||||||  | ||| ||  ||| ||    ||| ||| ||||||||||||||||||
Sbjct: 122 tttcggagagggaggagaggagctagactctcacggtggtc-gtctttgagataggcttg 180

                                                                       
Query: 175 tgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggg 234
           || |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 181 tggtttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggg 240

                                   
Query: 235 agctctgcatcctgatccatccat 258
           ||||||||||||||| ||||||||
Sbjct: 241 agctctgcatcctgaaccatccat 264




>54631_sof-miR159d:pmrd
          Length = 265

 Score =  276 bits (139), Expect = 8e-75
 Identities = 236/265 (89%), Gaps = 10/265 (3%)
 Strand = Plus / Plus

                                                                       
Query: 1   ctgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggt 60
           |||||||||||||| ||||||||||||||||||||||||||||||||| || | | ||||
Sbjct: 2   ctgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggt 61

                                                                       
Query: 61  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtac 120
            ||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| 
Sbjct: 62  tgttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtat 121

                                                                       
Query: 121 tctagag-ggccggagaagagat------tcatgtggtcgtcagtctttgagataggctt 173
           | | ||| ||  ||||| ||| |      ||||| ||  ||| |||| ||||||| ||||
Sbjct: 122 tttcgagagggaggagaggagctagactctcatgggg--gtc-gtctatgagatacgctt 178

                                                                       
Query: 174 gtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaagg 233
           ||| ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 179 gtggtttgcatgaccgatgagctgcaccgtccccttgctggccgctctttggattgaagg 238

                                    
Query: 234 gagctctgcatcctgatccatccat 258
           |||||||||||||||||||||||||
Sbjct: 239 gagctctgcatcctgatccatccat 263




>54632_sof-miR159e:pmrd
          Length = 264

 Score =  260 bits (131), Expect = 5e-70
 Identities = 235/264 (89%), Gaps = 8/264 (3%)
 Strand = Plus / Plus

                                                                       
Query: 1   ctgatgcttggattcgaagcggagctcctatcattccaatgaagggcccttttcatgggt 60
           |||||||||||||| ||||||||||||||||||||||||||||||||| || | | ||||
Sbjct: 1   ctgatgcttggatttgaagcggagctcctatcattccaatgaagggccgttctgaagggt 60

                                                                       
Query: 61  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatctgtgtatgtac 120
           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||| 
Sbjct: 61  ggttccgctgctcgttcatggttcccactatcctatctcatcatgtatgtgtgtatgtat 120

                                                                       
Query: 121 tctagag-ggccggagaag----agattcatgtggtcgtcagtctttgagataggcttgt 175
           | | ||| ||  ||||| |    ||| ||    ||| ||| |||||||||||||||||||
Sbjct: 121 tttcgagagggaggagaggagctagactctcacggtggtc-gtctttgagataggcttgt 179

                                                                       
Query: 176 gatttgcatgaccgaggagctgcaccgtccccttgctggccgctctttggattgaaggga 235
           | ||||||||||||| |||||||||||||||||||||||||||||||||||||||| |||
Sbjct: 180 ggtttgcatgaccgaagagctgcaccgtccccttgctggccgctctttggattgaaagga 239

                                   
Query: 236 gctc-tgcatcctgatccatccat 258
           |||| ||||| |||||||||||||
Sbjct: 240 gctcttgcat-ctgatccatccat 262




>54930_zma-miR159a:pmrd
          Length = 246

 Score =  176 bits (89), Expect = 6e-45
 Identities = 95/97 (97%)
 Strand = Plus / Plus

                                                                       
Query: 157 gtctttgagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggcc 216
           |||||||||||||||||||| ||||||||||||||||||||||||| |||||||||||||
Sbjct: 143 gtctttgagataggcttgtggtttgcatgaccgaggagctgcaccgcccccttgctggcc 202

                                                
Query: 217 gctctttggattgaagggagctctgcatcctgatcca 253
           |||||||||||||||||||||||||||||||||||||
Sbjct: 203 gctctttggattgaagggagctctgcatcctgatcca 239



 Score =  119 bits (60), Expect = 1e-27
 Identities = 85/92 (92%), Gaps = 1/92 (1%)
 Strand = Plus / Plus

                                                                       
Query: 16  gaagcggagctcctatcattccaatgaagggcccttttcatggg-tggttccgctgctcg 74
           ||||||||||||||||||||||||||||||| | ||   | ||| |||||||||||||||
Sbjct: 17  gaagcggagctcctatcattccaatgaagggtcgttccgaagggctggttccgctgctcg 76

                                           
Query: 75  ttcatggttcccactatcctatctcatcatgt 106
           ||||||||||||||||||||||||||||||||
Sbjct: 77  ttcatggttcccactatcctatctcatcatgt 108




>51338_pgl-miR159:pmrd
          Length = 226

 Score =  145 bits (73), Expect = 2e-35
 Identities = 79/81 (97%)
 Strand = Plus / Plus

                                                                       
Query: 161 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctc 220
           ||||||||||||||||  ||||||||||||||||||||||||||||||||||||||||||
Sbjct: 146 ttgagataggcttgtggcttgcatgaccgaggagctgcaccgtccccttgctggccgctc 205

                                
Query: 221 tttggattgaagggagctctg 241
           |||||||||||||||||||||
Sbjct: 206 tttggattgaagggagctctg 226



 Score = 85.7 bits (43), Expect = 2e-17
 Identities = 74/83 (89%), Gaps = 1/83 (1%)
 Strand = Plus / Plus

                                                                       
Query: 20  cggagctcctatcattccaatgaagggcccttttcatgggt-ggttccgctgctcgttca 78
           |||||||||||||||||||||||| ||   ||||  ||||| ||| | ||||||||||||
Sbjct: 1   cggagctcctatcattccaatgaaaggttgtttttgtgggttggtacagctgctcgttca 60

                                  
Query: 79  tggttcccactatcctatctcat 101
           |||||||||||||||||||||||
Sbjct: 61  tggttcccactatcctatctcat 83




>54392_sar-miR159:pmrd
          Length = 175

 Score =  109 bits (55), Expect = 1e-24
 Identities = 73/79 (92%)
 Strand = Plus / Plus

                                                                       
Query: 163 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 222
           ||||||||| ||||  ||||||||||||||||| ||| |||||||| |||||||||||||
Sbjct: 97  gagataggcctgtggcttgcatgaccgaggagccgcatcgtcccctcgctggccgctctt 156

                              
Query: 223 tggattgaagggagctctg 241
           |||||||||||||||||||
Sbjct: 157 tggattgaagggagctctg 175



 Score = 61.9 bits (31), Expect = 2e-10
 Identities = 37/39 (94%)
 Strand = Plus / Plus

                                                 
Query: 57 gggtggttccgctgctcgttcatggttcccactatccta 95
          ||||||| | |||||||||||||||||||||||||||||
Sbjct: 40 gggtggtacagctgctcgttcatggttcccactatccta 78




>54931_zma-miR159b:pmrd
          Length = 221

 Score = 99.6 bits (50), Expect = 1e-21
 Identities = 79/86 (91%), Gaps = 2/86 (2%)
 Strand = Plus / Plus

                                                                       
Query: 161 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtcccctt-gctggccgct 219
           ||||||| |||||||| |||||||||||||||||||||| ||||||||| | || |||||
Sbjct: 122 ttgagatgggcttgtggtttgcatgaccgaggagctgca-cgtccccttcgttgaccgct 180

                                     
Query: 220 ctttggattgaagggagctctgcatc 245
            |||||||||||||||||||||||||
Sbjct: 181 gtttggattgaagggagctctgcatc 206



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 45/49 (91%)
 Strand = Plus / Plus

                                                            
Query: 55  atgggtggttccgctgctcgttcatggttcccactatcctatctcatca 103
           ||||||||| | ||||||||||||||||||||||| ||| |||||||||
Sbjct: 56  atgggtggtacagctgctcgttcatggttcccactgtcccatctcatca 104




>48820_osa-miR159b:pmrd
          Length = 188

 Score = 89.7 bits (45), Expect = 1e-18
 Identities = 78/89 (87%)
 Strand = Plus / Plus

                                                                       
Query: 163 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 222
           |||||||||||||| ||||||||||| |||||| | | |  | |||||||| || |||||
Sbjct: 100 gagataggcttgtggtttgcatgaccaaggagccgaatcaactccttgctgaccactctt 159

                                        
Query: 223 tggattgaagggagctctgcatcctgatc 251
           ||||||||||||||||||||||| |||||
Sbjct: 160 tggattgaagggagctctgcatcttgatc 188



 Score = 54.0 bits (27), Expect = 6e-08
 Identities = 33/35 (94%)
 Strand = Plus / Plus

                                              
Query: 67  gctgctcgttcatggttcccactatcctatctcat 101
           |||||| |||||||||||||||||| |||||||||
Sbjct: 58  gctgcttgttcatggttcccactattctatctcat 92




>54934_zma-miR159e:pmrd
          Length = 203

 Score = 85.7 bits (43), Expect = 2e-17
 Identities = 74/83 (89%), Gaps = 1/83 (1%)
 Strand = Plus / Plus

                                                                       
Query: 161 ttgagataggcttgtgatttgcatgaccgaggagctgcaccgtcccctt-gctggccgct 219
           ||||||| |||||||| |||||||||||||||||||||  ||||||||| | || |||||
Sbjct: 121 ttgagatgggcttgtggtttgcatgaccgaggagctgcctcgtccccttcgttgaccgct 180

                                  
Query: 220 ctttggattgaagggagctctgc 242
            |||||| |||||||||||||||
Sbjct: 181 gtttggagtgaagggagctctgc 203



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 45/49 (91%)
 Strand = Plus / Plus

                                                            
Query: 55  atgggtggttccgctgctcgttcatggttcccactatcctatctcatca 103
           ||||||||| | ||||||||||||||||||||||| ||| |||||||||
Sbjct: 38  atgggtggtacagctgctcgttcatggttcccactgtcccatctcatca 86




>54653_spr-miR159:pmrd
          Length = 392

 Score = 77.8 bits (39), Expect = 4e-15
 Identities = 39/39 (100%)
 Strand = Plus / Plus

                                                  
Query: 221 tttggattgaagggagctctgcatcctgatccatccatt 259
           |||||||||||||||||||||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctctgcatcctgatccatccatt 39




>48657_hvv-miR159b:pmrd
          Length = 174

 Score = 77.8 bits (39), Expect = 4e-15
 Identities = 69/79 (87%)
 Strand = Plus / Plus

                                                                       
Query: 163 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 222
           |||||||||||||| ||||||||| |||||||| ||  ||  |||| |||| ||||| ||
Sbjct: 96  gagataggcttgtggtttgcatgatcgaggagccgcttcgatccctcgctgaccgctgtt 155

                              
Query: 223 tggattgaagggagctctg 241
           |||||||||||||||||||
Sbjct: 156 tggattgaagggagctctg 174



 Score = 58.0 bits (29), Expect = 4e-09
 Identities = 32/33 (96%)
 Strand = Plus / Plus

                                           
Query: 67 gctgctcgttcatggttcccactatcctatctc 99
          |||||| ||||||||||||||||||||||||||
Sbjct: 49 gctgcttgttcatggttcccactatcctatctc 81



 Score = 52.0 bits (26), Expect = 2e-07
 Identities = 26/26 (100%)
 Strand = Plus / Plus

                                    
Query: 21 ggagctcctatcattccaatgaaggg 46
          ||||||||||||||||||||||||||
Sbjct: 2  ggagctcctatcattccaatgaaggg 27




>54673_tae-miR159a:pmrd
          Length = 177

 Score = 75.8 bits (38), Expect = 2e-14
 Identities = 71/82 (86%)
 Strand = Plus / Plus

                                                                       
Query: 163 gagataggcttgtgatttgcatgaccgaggagctgcaccgtccccttgctggccgctctt 222
           |||| |||| |||| |||||||||||||||||| ||  ||  |||| |||| ||||| ||
Sbjct: 96  gagagaggcctgtggtttgcatgaccgaggagccgcttcgatccctcgctgaccgctgtt 155

                                 
Query: 223 tggattgaagggagctctgcat 244
           ||||||||||||||||||||||
Sbjct: 156 tggattgaagggagctctgcat 177



 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 33/33 (100%)
 Strand = Plus / Plus

                                           
Query: 67 gctgctcgttcatggttcccactatcctatctc 99
          |||||||||||||||||||||||||||||||||
Sbjct: 50 gctgctcgttcatggttcccactatcctatctc 82




>54937_zma-miR159m:pmrd
          Length = 457

 Score = 65.9 bits (33), Expect = 1e-11
 Identities = 33/33 (100%)
 Strand = Plus / Plus

                                            
Query: 221 tttggattgaagggagctctgcatcctgatcca 253
           |||||||||||||||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctctgcatcctgatcca 33




>54674_tae-miR159b:pmrd
          Length = 255

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 218 ctctttggattgaagggagctctgcatc 245
           ||||||||||||||||||||||||||||
Sbjct: 227 ctctttggattgaagggagctctgcatc 254




>48819_osa-miR159a:pmrd
          Length = 272

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 218 ctctttggattgaagggagctctgcatc 245
           ||||||||||||||||||||||||||||
Sbjct: 239 ctctttggattgaagggagctctgcatc 266




>48796_oru-miR159:pmrd
          Length = 274

 Score = 56.0 bits (28), Expect = 1e-08
 Identities = 28/28 (100%)
 Strand = Plus / Plus

                                       
Query: 218 ctctttggattgaagggagctctgcatc 245
           ||||||||||||||||||||||||||||
Sbjct: 239 ctctttggattgaagggagctctgcatc 266




>48656_hvv-miR159a:pmrd
          Length = 303

 Score = 54.0 bits (27), Expect = 6e-08
 Identities = 30/31 (96%)
 Strand = Plus / Plus

                                          
Query: 221 tttggattgaagggagctctgcatcctgatc 251
           ||||||||||||||||||||||||| |||||
Sbjct: 1   tttggattgaagggagctctgcatcttgatc 31




>48825_osa-miR159m:pmrd
          Length = 249

 Score = 48.1 bits (24), Expect = 3e-06
 Identities = 24/24 (100%)
 Strand = Plus / Plus

                                   
Query: 218 ctctttggattgaagggagctctg 241
           ||||||||||||||||||||||||
Sbjct: 226 ctctttggattgaagggagctctg 249




>46932_ath-miR159b:pmrd
          Length = 196

 Score = 46.1 bits (23), Expect = 1e-05
 Identities = 26/27 (96%)
 Strand = Plus / Plus

                                      
Query: 219 tctttggattgaagggagctctgcatc 245
           |||||||||||||||||||||| ||||
Sbjct: 166 tctttggattgaagggagctcttcatc 192




>54378_pvu-miR159:pmrd
          Length = 174

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 151 tctttggattgaagggagctct 172




>54377_pvu-miR159:pmrd
          Length = 174

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 151 tctttggattgaagggagctct 172




>48699_mtr-miR159a:pmrd
          Length = 168

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 146 tctttggattgaagggagctct 167




>48539_gma-miR159a:pmrd
          Length = 214

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 173 tctttggattgaagggagctct 194




>48395_bra-miR159a:pmrd
          Length = 185

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 161 tctttggattgaagggagctct 182




>48339_bna-miR159:pmrd
          Length = 183

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 159 tctttggattgaagggagctct 180




>46931_ath-miR159a:pmrd
          Length = 184

 Score = 44.1 bits (22), Expect = 5e-05
 Identities = 22/22 (100%)
 Strand = Plus / Plus

                                 
Query: 219 tctttggattgaagggagctct 240
           ||||||||||||||||||||||
Sbjct: 161 tctttggattgaagggagctct 182




>48701_mtr-miR159c:pmrd
          Length = 44

 Score = 42.1 bits (21), Expect = 2e-04
 Identities = 24/25 (96%)
 Strand = Plus / Plus

                                    
Query: 221 tttggattgaagggagctctgcatc 245
           |||||||||||||||||||| ||||
Sbjct: 1   tttggattgaagggagctctacatc 25




>54777_vvi-miR159c:pmrd
          Length = 190

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 160 tttggattgaagggagctct 179




>54540_sly-miR159:pmrd
          Length = 177

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 157 tttggattgaagggagctct 176




>52093_ptc-miRf10271-akr:pmrd
          Length = 174

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 149 tttggattgaagggagctct 168




>51604_ptc-miR159c:pmrd
          Length = 183

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 154 tttggattgaagggagctct 173




>51603_ptc-miR159b:pmrd
          Length = 187

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 158 tttggattgaagggagctct 177




>51602_ptc-miR159a:pmrd
          Length = 183

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 154 tttggattgaagggagctct 173




>48728_mtr-miR319b:pmrd
          Length = 42

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 23/24 (95%)
 Strand = Plus / Plus

                                   
Query: 222 ttggattgaagggagctctgcatc 245
           ||||||||||||||||||| ||||
Sbjct: 1   ttggattgaagggagctctacatc 24




>48686_ltu-miR159:pmrd
          Length = 345

 Score = 40.1 bits (20), Expect = 8e-04
 Identities = 20/20 (100%)
 Strand = Plus / Plus

                               
Query: 221 tttggattgaagggagctct 240
           ||||||||||||||||||||
Sbjct: 1   tttggattgaagggagctct 20