miRNEST 2.0, an integrative microRNA resource :: BLAST
>41439_ptc-MIR396e:mirbase
Length = 145
Score = 161 bits (81), Expect = 2e-40
Identities = 81/81 (100%)
Strand = Plus / Plus
Query: 2 ggtcatgcttttccacagctttcttgaacttctttgccttgcttaatctgtgtatatata 61
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct: 1 ggtcatgcttttccacagctttcttgaacttctttgccttgcttaatctgtgtatatata 60
Query: 62 gatcactacatgtacagctcc 82
|||||||||||||||||||||
Sbjct: 61 gatcactacatgtacagctcc 81
Score = 56.0 bits (28), Expect = 7e-09
Identities = 28/28 (100%)
Strand = Plus / Plus
Query: 109 gcgccatggaagctcaagaaagctgtgg 136
||||||||||||||||||||||||||||
Sbjct: 108 gcgccatggaagctcaagaaagctgtgg 135
>41438_ptc-MIR396d:mirbase
Length = 154
Score = 75.8 bits (38), Expect = 7e-15
Identities = 44/46 (95%)
Strand = Plus / Plus
Query: 2 ggtcatgcttttccacagctttcttgaacttctttgccttgcttaa 47
|||||||||||||||||||||||||||||||| ||||| |||||||
Sbjct: 1 ggtcatgcttttccacagctttcttgaacttccttgccatgcttaa 46
Score = 42.1 bits (21), Expect = 1e-04
Identities = 24/25 (96%)
Strand = Plus / Plus
Query: 112 ccatggaagctcaagaaagctgtgg 136
|||||||||||||||||||| ||||
Sbjct: 120 ccatggaagctcaagaaagccgtgg 144
>40474_gma-MIR396b:mirbase
Length = 126
Score = 69.9 bits (35), Expect = 5e-13
Identities = 35/35 (100%)
Strand = Plus / Plus
Query: 1 tggtcatgcttttccacagctttcttgaacttctt 35
|||||||||||||||||||||||||||||||||||
Sbjct: 10 tggtcatgcttttccacagctttcttgaacttctt 44
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 117 gaagctcaagaaagctgtgg 136
||||||||||||||||||||
Sbjct: 86 gaagctcaagaaagctgtgg 105
>40476_gma-MIR396d:mirbase
Length = 108
Score = 67.9 bits (34), Expect = 2e-12
Identities = 34/34 (100%)
Strand = Plus / Plus
Query: 2 ggtcatgcttttccacagctttcttgaacttctt 35
||||||||||||||||||||||||||||||||||
Sbjct: 1 ggtcatgcttttccacagctttcttgaacttctt 34
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 117 gaagctcaagaaagctgtgg 136
||||||||||||||||||||
Sbjct: 76 gaagctcaagaaagctgtgg 95
>41212_ccl-MIR396:mirbase
Length = 277
Score = 65.9 bits (33), Expect = 7e-12
Identities = 33/33 (100%)
Strand = Plus / Plus
Query: 1 tggtcatgcttttccacagctttcttgaacttc 33
|||||||||||||||||||||||||||||||||
Sbjct: 132 tggtcatgcttttccacagctttcttgaacttc 164
Score = 48.1 bits (24), Expect = 2e-06
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 109 gcgccatggaagctcaagaaagctgtgg 136
||||||||| ||||||||||||||||||
Sbjct: 223 gcgccatgggagctcaagaaagctgtgg 250
>41661_vvi-MIR396b:mirbase
Length = 108
Score = 61.9 bits (31), Expect = 1e-10
Identities = 31/31 (100%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaacttctt 35
|||||||||||||||||||||||||||||||
Sbjct: 7 catgcttttccacagctttcttgaacttctt 37
Score = 42.1 bits (21), Expect = 1e-04
Identities = 27/29 (93%)
Strand = Plus / Minus
Query: 5 catgcttttccacagctttcttgaacttc 33
|||| ||| ||||||||||||||||||||
Sbjct: 104 catggtttcccacagctttcttgaacttc 76
>41660_vvi-MIR396a:mirbase
Length = 116
Score = 60.0 bits (30), Expect = 4e-10
Identities = 33/34 (97%)
Strand = Plus / Plus
Query: 3 gtcatgcttttccacagctttcttgaacttcttt 36
||||||||||||||||||||||||||||| ||||
Sbjct: 5 gtcatgcttttccacagctttcttgaactacttt 38
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 117 gaagctcaagaaagctgtgg 136
||||||||||||||||||||
Sbjct: 84 gaagctcaagaaagctgtgg 103
>41243_csi-MIR396c:mirbase
Length = 178
Score = 60.0 bits (30), Expect = 4e-10
Identities = 33/34 (97%)
Strand = Plus / Plus
Query: 3 gtcatgcttttccacagctttcttgaacttcttt 36
|||||| |||||||||||||||||||||||||||
Sbjct: 4 gtcatgtttttccacagctttcttgaacttcttt 37
>40726_mtr-MIR396a:mirbase
Length = 93
Score = 60.0 bits (30), Expect = 4e-10
Identities = 30/30 (100%)
Strand = Plus / Plus
Query: 7 tgcttttccacagctttcttgaacttcttt 36
||||||||||||||||||||||||||||||
Sbjct: 1 tgcttttccacagctttcttgaacttcttt 30
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 117 gaagctcaagaaagctgtgg 136
||||||||||||||||||||
Sbjct: 67 gaagctcaagaaagctgtgg 86
>40475_gma-MIR396c:mirbase
Length = 117
Score = 60.0 bits (30), Expect = 4e-10
Identities = 30/30 (100%)
Strand = Plus / Plus
Query: 6 atgcttttccacagctttcttgaacttctt 35
||||||||||||||||||||||||||||||
Sbjct: 15 atgcttttccacagctttcttgaacttctt 44
>40379_rco-MIR396:mirbase
Length = 124
Score = 58.0 bits (29), Expect = 2e-09
Identities = 29/29 (100%)
Strand = Plus / Plus
Query: 7 tgcttttccacagctttcttgaacttctt 35
|||||||||||||||||||||||||||||
Sbjct: 1 tgcttttccacagctttcttgaacttctt 29
>41142_tcc-MIR396e:mirbase
Length = 146
Score = 56.0 bits (28), Expect = 7e-09
Identities = 31/32 (96%)
Strand = Plus / Minus
Query: 3 gtcatgcttttccacagctttcttgaacttct 34
||||||||||||||| ||||||||||||||||
Sbjct: 133 gtcatgcttttccacggctttcttgaacttct 102
Score = 46.1 bits (23), Expect = 7e-06
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaacttct 34
|||||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaacttct 43
>41140_tcc-MIR396c:mirbase
Length = 152
Score = 56.0 bits (28), Expect = 7e-09
Identities = 28/28 (100%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaactt 32
||||||||||||||||||||||||||||
Sbjct: 14 catgcttttccacagctttcttgaactt 41
Score = 42.1 bits (21), Expect = 1e-04
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 116 ggaagctcaagaaagctgtgg 136
|||||||||||||||||||||
Sbjct: 108 ggaagctcaagaaagctgtgg 128
>40086_ath-MIR396b:mirbase
Length = 135
Score = 54.0 bits (27), Expect = 3e-08
Identities = 30/31 (96%)
Strand = Plus / Plus
Query: 2 ggtcatgcttttccacagctttcttgaactt 32
|||||| ||||||||||||||||||||||||
Sbjct: 1 ggtcatacttttccacagctttcttgaactt 31
Score = 44.1 bits (22), Expect = 3e-05
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 115 tggaagctcaagaaagctgtgg 136
||||||||||||||||||||||
Sbjct: 102 tggaagctcaagaaagctgtgg 123
>42584_sbi-MIR396c:mirbase
Length = 162
Score = 52.0 bits (26), Expect = 1e-07
Identities = 29/30 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaacttct 34
||||| ||||||||||||||||||||||||
Sbjct: 14 catgcctttccacagctttcttgaacttct 43
Score = 40.1 bits (20), Expect = 4e-04
Identities = 23/24 (95%)
Strand = Plus / Minus
Query: 14 ccacagctttcttgaacttctttg 37
||||||||||||||| ||||||||
Sbjct: 141 ccacagctttcttgaccttctttg 118
>42028_osa-MIR396c:mirbase
Length = 141
Score = 52.0 bits (26), Expect = 1e-07
Identities = 29/30 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaacttct 34
||||| ||||||||||||||||||||||||
Sbjct: 4 catgcctttccacagctttcttgaacttct 33
>39426_pta-MIR396:mirbase
Length = 119
Score = 52.0 bits (26), Expect = 1e-07
Identities = 29/30 (96%)
Strand = Plus / Plus
Query: 3 gtcatgcttttccacagctttcttgaactt 32
||||| ||||||||||||||||||||||||
Sbjct: 8 gtcatacttttccacagctttcttgaactt 37
Score = 40.1 bits (20), Expect = 4e-04
Identities = 26/28 (92%)
Strand = Plus / Minus
Query: 5 catgcttttccacagctttcttgaactt 32
|||||||| |||||| ||||||||||||
Sbjct: 110 catgctttcccacagttttcttgaactt 83
>42812_zma-MIR396f:mirbase
Length = 117
Score = 50.1 bits (25), Expect = 4e-07
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 11 tttccacagctttcttgaacttctt 35
|||||||||||||||||||||||||
Sbjct: 1 tttccacagctttcttgaacttctt 25
>42811_zma-MIR396e:mirbase
Length = 166
Score = 50.1 bits (25), Expect = 4e-07
Identities = 25/25 (100%)
Strand = Plus / Plus
Query: 11 tttccacagctttcttgaacttctt 35
|||||||||||||||||||||||||
Sbjct: 1 tttccacagctttcttgaacttctt 25
>41437_ptc-MIR396c:mirbase
Length = 102
Score = 50.1 bits (25), Expect = 4e-07
Identities = 28/29 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaacttc 33
|||| ||||||||||||||||||||||||
Sbjct: 4 catgtttttccacagctttcttgaacttc 32
Score = 48.1 bits (24), Expect = 2e-06
Identities = 30/32 (93%)
Strand = Plus / Minus
Query: 4 tcatgcttttccacagctttcttgaacttctt 35
||||||| |||||| |||||||||||||||||
Sbjct: 102 tcatgctattccacggctttcttgaacttctt 71
>41441_ptc-MIR396g:mirbase
Length = 168
Score = 48.1 bits (24), Expect = 2e-06
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaactt 32
||||||||||||| ||||||||||||||
Sbjct: 4 catgcttttccacggctttcttgaactt 31
>41141_tcc-MIR396d:mirbase
Length = 182
Score = 48.1 bits (24), Expect = 2e-06
Identities = 27/28 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaactt 32
||||||||||||| ||||||||||||||
Sbjct: 14 catgcttttccacggctttcttgaactt 41
>39878_aly-MIR396b:mirbase
Length = 188
Score = 48.1 bits (24), Expect = 2e-06
Identities = 30/32 (93%)
Strand = Plus / Plus
Query: 1 tggtcatgcttttccacagctttcttgaactt 32
||||||| |||||||||||||||||||||||
Sbjct: 21 tggtcatatttttccacagctttcttgaactt 52
Score = 42.1 bits (21), Expect = 1e-04
Identities = 30/33 (90%)
Strand = Plus / Minus
Query: 3 gtcatgcttttccacagctttcttgaacttctt 35
|||||| ||| ||||||||||||||| ||||||
Sbjct: 162 gtcatgttttcccacagctttcttgagcttctt 130
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 117 gaagctcaagaaagctgtgg 136
||||||||||||||||||||
Sbjct: 132 gaagctcaagaaagctgtgg 151
>41795_bdi-MIR396e:mirbase
Length = 201
Score = 46.1 bits (23), Expect = 7e-06
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 11 tttccacagctttcttgaacttc 33
|||||||||||||||||||||||
Sbjct: 31 tttccacagctttcttgaacttc 53
>39741_smo-MIR396:mirbase
Length = 168
Score = 46.1 bits (23), Expect = 7e-06
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 4 tcatgcttttccacagctttcttgaac 30
|||||||||||||| ||||||||||||
Sbjct: 3 tcatgcttttccacggctttcttgaac 29
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 14 ccacagctttcttgaacttc 33
||||||||||||||||||||
Sbjct: 153 ccacagctttcttgaacttc 134
>39379_pab-MIR396a:mirbase
Length = 100
Score = 46.1 bits (23), Expect = 7e-06
Identities = 26/27 (96%)
Strand = Plus / Plus
Query: 5 catgcttttccacagctttcttgaact 31
|||||| ||||||||||||||||||||
Sbjct: 9 catgctcttccacagctttcttgaact 35
>41242_csi-MIR396b:mirbase
Length = 134
Score = 44.1 bits (22), Expect = 3e-05
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 10 ttttccacagctttcttgaact 31
||||||||||||||||||||||
Sbjct: 18 ttttccacagctttcttgaact 39
>41138_tcc-MIR396a:mirbase
Length = 146
Score = 44.1 bits (22), Expect = 3e-05
Identities = 22/22 (100%)
Strand = Plus / Plus
Query: 10 ttttccacagctttcttgaact 31
||||||||||||||||||||||
Sbjct: 19 ttttccacagctttcttgaact 40
>41189_aqc-MIR396b:mirbase
Length = 89
Score = 42.1 bits (21), Expect = 1e-04
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaactt 32
|||||||||||||||||||||
Sbjct: 1 ttccacagctttcttgaactt 21
>40296_bna-MIR396a:mirbase
Length = 117
Score = 42.1 bits (21), Expect = 1e-04
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaactt 32
|||||||||||||||||||||
Sbjct: 1 ttccacagctttcttgaactt 21
>42814_zma-MIR396h:mirbase
Length = 124
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 98 ttccacagctttcttgaact 79
>42813_zma-MIR396g:mirbase
Length = 114
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 111 ttccacagctttcttgaact 92
>42808_zma-MIR396b:mirbase
Length = 136
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>42807_zma-MIR396a:mirbase
Length = 137
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>42583_sbi-MIR396b:mirbase
Length = 128
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>42582_sbi-MIR396a:mirbase
Length = 125
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>42472_ssp-MIR396:mirbase
Length = 95
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 1 ttccacagctttcttgaact 20
>42461_sof-MIR396:mirbase
Length = 134
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>42027_osa-MIR396b:mirbase
Length = 116
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>42026_osa-MIR396a:mirbase
Length = 154
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41794_bdi-MIR396d:mirbase
Length = 152
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 33 ttccacagctttcttgaact 52
>41793_bdi-MIR396c:mirbase
Length = 153
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 33 ttccacagctttcttgaact 52
>41663_vvi-MIR396d:mirbase
Length = 120
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41662_vvi-MIR396c:mirbase
Length = 108
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41436_ptc-MIR396b:mirbase
Length = 132
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41435_ptc-MIR396a:mirbase
Length = 123
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41241_csi-MIR396a:mirbase
Length = 153
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Minus
Query: 10 ttttccacagctttcttgaa 29
||||||||||||||||||||
Sbjct: 109 ttttccacagctttcttgaa 90
>41188_aqc-MIR396a:mirbase
Length = 122
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 1 ttccacagctttcttgaact 20
>41139_tcc-MIR396b:mirbase
Length = 151
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>41058_ghr-MIR396b:mirbase
Length = 135
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>41057_ghr-MIR396a:mirbase
Length = 136
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>40727_mtr-MIR396b:mirbase
Length = 95
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 6 ttccacagctttcttgaact 25
>40646_lja-MIR396:mirbase
Length = 108
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 16 ttccacagctttcttgaact 35
>40477_gma-MIR396e:mirbase
Length = 114
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 7 ttccacagctttcttgaact 26
>40473_gma-MIR396a:mirbase
Length = 140
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 21 ttccacagctttcttgaact 40
>40085_ath-MIR396a:mirbase
Length = 151
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 11 ttccacagctttcttgaact 30
>39877_aly-MIR396a:mirbase
Length = 172
Score = 40.1 bits (20), Expect = 4e-04
Identities = 20/20 (100%)
Strand = Plus / Plus
Query: 12 ttccacagctttcttgaact 31
||||||||||||||||||||
Sbjct: 24 ttccacagctttcttgaact 43
>39381_pab-MIR396c:mirbase
Length = 143
Score = 40.1 bits (20), Expect = 4e-04
Identities = 23/24 (95%)
Strand = Plus / Plus
Query: 10 ttttccacagctttcttgaacttc 33
|||||||| |||||||||||||||
Sbjct: 23 ttttccacggctttcttgaacttc 46