miRNEST 2.0, an integrative microRNA resource :: BLAST
BLASTN 2.2.20 [Feb-08-2009]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
>34171_dre-let-7b:mirbase
Length = 94
Score = 155 bits (78), Expect = 7e-39
Identities = 87/90 (96%)
Strand = Plus / Plus
Query: 5 acagggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagata 64
|||||||||||||||||||||||||||||||||||||| ||||||||||||||||| ||
Sbjct: 5 acagggtgaggtagtaggttgtgtggtttcagggttgtgtttttgccccatcaggagtta 64
Query: 65 actatacaacctactgccttccctgaaggg 94
||||||||||||||||||||||||||||||
Sbjct: 65 actatacaacctactgccttccctgaaggg 94
>34528_fru-let-7b:mirbase
Length = 84
Score = 151 bits (76), Expect = 1e-37
Identities = 82/84 (97%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataa 65
||||||||||||||||||||||||||||||||||||| |||||||||||||||||| |||
Sbjct: 1 cagggtgaggtagtaggttgtgtggtttcagggttgtgattttgccccatcaggagctaa 60
Query: 66 ctatacaacctactgccttccctg 89
||||||||||||||||||||||||
Sbjct: 61 ctatacaacctactgccttccctg 84
>34676_tni-let-7b:mirbase
Length = 84
Score = 151 bits (76), Expect = 1e-37
Identities = 82/84 (97%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataa 65
||||||||||||||||||||||||||||||||||||| |||||||||||||||||| |||
Sbjct: 1 cagggtgaggtagtaggttgtgtggtttcagggttgtgattttgccccatcaggagctaa 60
Query: 66 ctatacaacctactgccttccctg 89
||||||||||||||||||||||||
Sbjct: 61 ctatacaacctactgccttccctg 84
>26379_tgu-let-7b:mirbase
Length = 92
Score = 133 bits (67), Expect = 2e-32
Identities = 83/87 (95%), Gaps = 1/87 (1%)
Strand = Plus / Plus
Query: 5 acagggtgaggtagtaggttgtgtggtttcagggttgtaattttgcccc-atcaggagat 63
||||| ||||||||||||||||||||||||||||| || |||||||||| ||||||||||
Sbjct: 5 acaggatgaggtagtaggttgtgtggtttcagggtagtgattttgccccaatcaggagat 64
Query: 64 aactatacaacctactgccttccctga 90
|||||||||||||||||||||||||||
Sbjct: 65 aactatacaacctactgccttccctga 91
>25871_gga-let-7b:mirbase
Length = 85
Score = 129 bits (65), Expect = 4e-31
Identities = 81/85 (95%), Gaps = 1/85 (1%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggtttcagggttgtaattttgcccc-atcaggagata 64
|||| ||||||||||||||||||||||||||||| || |||||||||| |||||||||||
Sbjct: 1 caggatgaggtagtaggttgtgtggtttcagggtagtgattttgccccaatcaggagata 60
Query: 65 actatacaacctactgccttccctg 89
|||||||||||||||||||||||||
Sbjct: 61 actatacaacctactgccttccctg 85
>27321_mdo-let-7b:mirbase
Length = 88
Score = 127 bits (64), Expect = 2e-30
Identities = 80/84 (95%), Gaps = 1/84 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgcccc-atcaggagataac 66
|||||||||||||||||||||||||||||||| || |||||||||| ||||| |||||||
Sbjct: 5 gggtgaggtagtaggttgtgtggtttcagggtagtgattttgccccaatcagaagataac 64
Query: 67 tatacaacctactgccttccctga 90
||||||||||||||||||||||||
Sbjct: 65 tatacaacctactgccttccctga 88
>33111_bta-let-7b:mirbase
Length = 81
Score = 109 bits (55), Expect = 4e-25
Identities = 73/79 (92%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
|||||||||||||||||||||||||||||||| || || ||||||| || | ||||||||
Sbjct: 3 gggtgaggtagtaggttgtgtggtttcagggtagtgatgttgccccctcggaagataact 62
Query: 68 atacaacctactgccttcc 86
|||||||||||||||||||
Sbjct: 63 atacaacctactgccttcc 81
>27632_mml-let-7b:mirbase
Length = 83
Score = 107 bits (54), Expect = 1e-24
Identities = 76/82 (92%), Gaps = 1/82 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
||||||||||||||||||||||||||||||| || || ||||||| |||| ||||||||
Sbjct: 3 gggtgaggtagtaggttgtgtggtttcagggcagtgatgttgcccc-tcagaagataact 61
Query: 68 atacaacctactgccttccctg 89
||||||||||||||||||||||
Sbjct: 62 atacaacctactgccttccctg 83
>30129_ptr-let-7b:mirbase
Length = 82
Score = 107 bits (54), Expect = 1e-24
Identities = 76/82 (92%), Gaps = 1/82 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
||||||||||||||||||||||||||||||| || || ||||||| |||| ||||||||
Sbjct: 2 gggtgaggtagtaggttgtgtggtttcagggcagtgatgttgcccc-tcagaagataact 60
Query: 68 atacaacctactgccttccctg 89
||||||||||||||||||||||
Sbjct: 61 atacaacctactgccttccctg 82
>30734_ppy-let-7b:mirbase
Length = 83
Score = 107 bits (54), Expect = 1e-24
Identities = 76/82 (92%), Gaps = 1/82 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
||||||||||||||||||||||||||||||| || || ||||||| |||| ||||||||
Sbjct: 3 gggtgaggtagtaggttgtgtggtttcagggcagtgatgttgcccc-tcagaagataact 61
Query: 68 atacaacctactgccttccctg 89
||||||||||||||||||||||
Sbjct: 62 atacaacctactgccttccctg 83
>33789_bta-mir-3596:mirbase
Length = 83
Score = 107 bits (54), Expect = 1e-24
Identities = 72/78 (92%)
Strand = Plus / Minus
Query: 9 ggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataacta 68
||||||||||||||||||||||||||||||| || || ||||||| || | |||||||||
Sbjct: 83 ggtgaggtagtaggttgtgtggtttcagggtagtgatgttgccccctcggaagataacta 24
Query: 69 tacaacctactgccttcc 86
||||||||||||||||||
Sbjct: 23 tacaacctactgccttcc 6
>31754_mmu-let-7b:mirbase
Length = 85
Score = 105 bits (53), Expect = 6e-24
Identities = 78/85 (91%), Gaps = 1/85 (1%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataa 65
||||||||||||||||||||||||||||||||| || || ||||||| || | ||||||
Sbjct: 2 cagggtgaggtagtaggttgtgtggtttcagggcagtgatgttgcccc-tccgaagataa 60
Query: 66 ctatacaacctactgccttccctga 90
|||||||||||||||||||||||||
Sbjct: 61 ctatacaacctactgccttccctga 85
>28342_hsa-let-7b:mirbase
Length = 83
Score = 99.6 bits (50), Expect = 3e-22
Identities = 75/82 (91%), Gaps = 1/82 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
||||||||||||||||||||||||||||||| || || ||||||| || | ||||||||
Sbjct: 3 gggtgaggtagtaggttgtgtggtttcagggcagtgatgttgcccc-tcggaagataact 61
Query: 68 atacaacctactgccttccctg 89
||||||||||||||||||||||
Sbjct: 62 atacaacctactgccttccctg 83
>31410_oan-let-7b:mirbase
Length = 82
Score = 99.6 bits (50), Expect = 3e-22
Identities = 72/78 (92%), Gaps = 1/78 (1%)
Strand = Plus / Plus
Query: 11 tgaggtagtaggttgtgtggtttcagggttgtaattttgcccc-atcaggagataactat 69
|||||||||||||||||||||||||||| || |||||||||| ||||| ||||||||||
Sbjct: 5 tgaggtagtaggttgtgtggtttcaggggagtgattttgccccaatcagaagataactat 64
Query: 70 acaacctactgccttccc 87
||| ||||||||||||||
Sbjct: 65 acagcctactgccttccc 82
>32640_rno-let-7b:mirbase
Length = 85
Score = 93.7 bits (47), Expect = 2e-20
Identities = 75/83 (90%), Gaps = 1/83 (1%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggttgtaattttgccccatcaggagataact 67
||||||||||||||||||||||||||||||| || || | ||||| || | ||||||||
Sbjct: 4 gggtgaggtagtaggttgtgtggtttcagggcagtgatgtcgcccc-tccgaagataact 62
Query: 68 atacaacctactgccttccctga 90
|||||||||||||||||||||||
Sbjct: 63 atacaacctactgccttccctga 85
>26607_cfa-let-7b:mirbase
Length = 69
Score = 61.9 bits (31), Expect = 7e-11
Identities = 31/31 (100%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcaggg 38
|||||||||||||||||||||||||||||||
Sbjct: 37 gggtgaggtagtaggttgtgtggtttcaggg 67
>25874_gga-let-7f:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>26384_tgu-let-7f:mirbase
Length = 92
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 56 tcaggagataactatacaatctattgccttccctga 91
>26934_eca-let-7f:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>27323_mdo-let-7f-1:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>27636_mml-let-7f-1:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>28346_hsa-let-7f-1:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>30133_ptr-let-7f-1:mirbase
Length = 86
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 51 tcaggagataactatacaatctattgccttccctga 86
>30738_ppy-let-7f-1:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>33115_bta-let-7f-1:mirbase
Length = 87
Score = 56.0 bits (28), Expect = 5e-09
Identities = 34/36 (94%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||||||||||||||||| ||| ||||||||||||
Sbjct: 52 tcaggagataactatacaatctattgccttccctga 87
>34532_fru-let-7h:mirbase
Length = 84
Score = 56.0 bits (28), Expect = 5e-09
Identities = 31/32 (96%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttcc 86
|||||||||||||||||||| |||||||||||
Sbjct: 52 tcaggagataactatacaacttactgccttcc 83
>31759_mmu-let-7f-1:mirbase
Length = 89
Score = 52.0 bits (26), Expect = 7e-08
Identities = 32/34 (94%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgccttccctga 90
||||||||||||||||| ||| ||||||||||||
Sbjct: 55 aggagataactatacaatctattgccttccctga 88
>32645_rno-let-7f-1:mirbase
Length = 89
Score = 52.0 bits (26), Expect = 7e-08
Identities = 32/34 (94%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgccttccctga 90
||||||||||||||||| ||| ||||||||||||
Sbjct: 55 aggagataactatacaatctattgccttccctga 88
>33101_rno-mir-3596d:mirbase
Length = 97
Score = 52.0 bits (26), Expect = 7e-08
Identities = 32/34 (94%)
Strand = Plus / Minus
Query: 57 aggagataactatacaacctactgccttccctga 90
||||||||||||||||| ||| ||||||||||||
Sbjct: 39 aggagataactatacaatctattgccttccctga 6
>34680_tni-let-7h:mirbase
Length = 99
Score = 52.0 bits (26), Expect = 7e-08
Identities = 29/30 (96%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgccttcc 86
|||||||||||||||||| |||||||||||
Sbjct: 69 aggagataactatacaacttactgccttcc 98
>25753_xtr-mir-98:mirbase
Length = 108
Score = 50.1 bits (25), Expect = 3e-07
Identities = 31/33 (93%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgccttccctg 89
|||||||||||||||| | ||||||||||||||
Sbjct: 64 aggagataactatacagcttactgccttccctg 96
>26610_cfa-let-7f:mirbase
Length = 78
Score = 50.1 bits (25), Expect = 3e-07
Identities = 31/33 (93%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccc 87
||||||||||||||||||| ||| |||||||||
Sbjct: 46 tcaggagataactatacaatctattgccttccc 78
>31413_oan-let-7f-1:mirbase
Length = 149
Score = 50.1 bits (25), Expect = 3e-07
Identities = 31/33 (93%)
Strand = Plus / Plus
Query: 58 ggagataactatacaacctactgccttccctga 90
|||||||||||||||| ||| ||||||||||||
Sbjct: 79 ggagataactatacaatctattgccttccctga 111
>34165_dre-let-7a-1:mirbase
Length = 89
Score = 46.1 bits (23), Expect = 4e-06
Identities = 23/23 (100%)
Strand = Plus / Plus
Query: 58 ggagataactatacaacctactg 80
|||||||||||||||||||||||
Sbjct: 53 ggagataactatacaacctactg 75
>34178_dre-let-7f:mirbase
Length = 116
Score = 46.1 bits (23), Expect = 4e-06
Identities = 29/31 (93%)
Strand = Plus / Plus
Query: 60 agataactatacaacctactgccttccctga 90
|||||||||||||| ||| ||||||||||||
Sbjct: 70 agataactatacaatctattgccttccctga 100
>31756_mmu-let-7c-2:mirbase
Length = 95
Score = 44.1 bits (22), Expect = 2e-05
Identities = 25/26 (96%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggttt 33
||||||||||||||||||| ||||||
Sbjct: 11 gggtgaggtagtaggttgtatggttt 36
>32642_rno-let-7c-2:mirbase
Length = 95
Score = 44.1 bits (22), Expect = 2e-05
Identities = 25/26 (96%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggttt 33
||||||||||||||||||| ||||||
Sbjct: 11 gggtgaggtagtaggttgtatggttt 36
>34181_dre-let-7h:mirbase
Length = 114
Score = 44.1 bits (22), Expect = 2e-05
Identities = 28/30 (93%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgccttcc 86
||||||||||||||||| |||||||||||
Sbjct: 70 aggagataactatacaatttactgccttcc 99
>25409_pma-let-7a-2:mirbase
Length = 103
Score = 42.1 bits (21), Expect = 7e-05
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 11 tgaggtagtaggttgtgtggtttcagggt 39
|||||||||||||||| | ||||||||||
Sbjct: 17 tgaggtagtaggttgtatagtttcagggt 45
>25870_gga-let-7a-3:mirbase
Length = 76
Score = 42.1 bits (21), Expect = 7e-05
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggtt 40
||||||||||||||||||| | |||| ||||||
Sbjct: 1 gggtgaggtagtaggttgtatagttttagggtt 33
>26375_tgu-let-7a-1:mirbase
Length = 92
Score = 42.1 bits (21), Expect = 7e-05
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 60 agataactatacaacctactgccttccctgaag 92
|||||||||||||| |||||| ||| |||||||
Sbjct: 55 agataactatacaatctactgtctttcctgaag 87
>34167_dre-let-7a-3:mirbase
Length = 107
Score = 42.1 bits (21), Expect = 7e-05
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggtt 40
||||||||||||||||||| | |||| ||||||
Sbjct: 15 gggtgaggtagtaggttgtatagtttgagggtt 47
>34173_dre-let-7c-2:mirbase
Length = 92
Score = 42.1 bits (21), Expect = 7e-05
Identities = 27/29 (93%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggtttc 34
|||| |||||||||||||||| |||||||
Sbjct: 10 caggctgaggtagtaggttgtatggtttc 38
>34526_fru-let-7a-2:mirbase
Length = 76
Score = 42.1 bits (21), Expect = 7e-05
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggtt 40
||||||||||||||||||| | |||| ||||||
Sbjct: 1 gggtgaggtagtaggttgtatagttttagggtt 33
>34673_tni-let-7a-1:mirbase
Length = 74
Score = 42.1 bits (21), Expect = 7e-05
Identities = 21/21 (100%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgt 26
|||||||||||||||||||||
Sbjct: 1 cagggtgaggtagtaggttgt 21
>34674_tni-let-7a-2:mirbase
Length = 84
Score = 42.1 bits (21), Expect = 7e-05
Identities = 30/33 (90%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggtt 40
||||||||||||||||||| | |||| ||||||
Sbjct: 9 gggtgaggtagtaggttgtatagttttagggtt 41
>25413_pma-let-7c:mirbase
Length = 96
Score = 40.1 bits (20), Expect = 3e-04
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 8 gggtgaggtagtaggttgtgtggtttcagggt 39
|||||||||||||| |||| |||||| |||||
Sbjct: 11 gggtgaggtagtagattgtatggttttagggt 42
>25678_xtr-let-7c:mirbase
Length = 94
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggttt 33
|||| |||||||||||||||| ||||||
Sbjct: 11 caggttgaggtagtaggttgtatggttt 38
>25680_xtr-let-7e-2:mirbase
Length = 93
Score = 40.1 bits (20), Expect = 3e-04
Identities = 29/32 (90%)
Strand = Plus / Plus
Query: 61 gataactatacaacctactgccttccctgaag 92
||||||||||||| |||||| ||| |||||||
Sbjct: 56 gataactatacaatctactgtctttcctgaag 87
>25873_gga-let-7d:mirbase
Length = 103
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgcctt 84
||||| |||||||||||||| |||||||
Sbjct: 61 aggaggtaactatacaacctgctgcctt 88
>26382_tgu-let-7d:mirbase
Length = 92
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgcctt 84
||||| |||||||||||||| |||||||
Sbjct: 56 aggaggtaactatacaacctgctgcctt 83
>26932_eca-let-7d:mirbase
Length = 87
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgcctt 84
||||||||||||||| |||| |||||||
Sbjct: 53 aggagataactatacgacctgctgcctt 80
>27322_mdo-let-7d:mirbase
Length = 106
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 57 aggagataactatacaacctactgcctt 84
||||| |||||||||||||| |||||||
Sbjct: 61 aggagttaactatacaacctgctgcctt 88
>34170_dre-let-7a-6:mirbase
Length = 129
Score = 40.1 bits (20), Expect = 3e-04
Identities = 32/36 (88%)
Strand = Plus / Plus
Query: 55 tcaggagataactatacaacctactgccttccctga 90
||||| |||||||||||| ||| ||||||||||||
Sbjct: 73 tcaggtgataactatacagtctattgccttccctga 108
>34172_dre-let-7c-1:mirbase
Length = 107
Score = 40.1 bits (20), Expect = 3e-04
Identities = 26/28 (92%)
Strand = Plus / Plus
Query: 6 cagggtgaggtagtaggttgtgtggttt 33
|||| |||||||||||||||| ||||||
Sbjct: 18 caggctgaggtagtaggttgtatggttt 45
