mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TATACTCCCTCCGTCCGAAAT TTTGGAACGGAGGGAGTATAAC ATATTAACTCATTTTGGAACGGAGGGAGTATAACATAAACTCTACTTACAACATAACCACAATTTATTATCATATACTCCCTCCGTCCGAAATTACTTATCG GACTTAAACTTCATATTAACTCATTTTGGAACGGAGGGAGTATAACATAAACTCTACTTACAACATAACCACAATTTATTATCATATACTCCCTCCGTCCGAAATTACTTATCGCCGAAATTAATTAAAATAGATGTATCCAGAACTAAAATACATCTAGATACATCCATTCCTTCAACAAGTATTTTCAGACAAAGGGAGTAGTTACAGACATGCACAGTGATGAGGCACAGAAACTACAACAATAAAAAGTATGCCAAGACATTTATTCTTTATTTTTCTTATTGTTGTATCAGCCCAGGTAACAAACTGGGAACGTGATGTAATGTCCAATACACACTACAGATAACACTTCCCTTGGAGGTCTTTTGAATACCACATATGGAAGTTGGTCGAGCAGTAGCTGTGCCAGGTTCCGATTATATGCAGCGCCTCACATGCGTGACCTCGTCGAAACTTGGTGAGCCGTCCGTGGCGCAGCCAGTGGTCGGCCGCTTTTCGCTCTTTCCTCCAAATACAATGATGGTTTGAAGAAATCACCGTACATTTCAGCCCACTTGCTAAGCTTTGAATGTATATAAGATGCTCCCACCGTGTCAGCCCAAAAGATGAGGCCACCCCTGTACTTGGGAAAGCCCATCCCAAGAACAGATGCAATATCCAGATCAGCAGCCCTGATCACCACATTTTCATCCATGACCCTGCATGCTTCATTAACGACTGGGAAGAAAACCATCTCCAATATATCTTGATCCGATAAAGTAACCGGCT 0 1 3' arm Hordeum_vulgare mirnest MIR1122 0.00009 tae-MIR1133 0.000001 -27.1 ..........((((((((.(((((((((((((..((((............................))))..)))))))))))))))))))))......... (-27.1)