mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGGACCAGGCTTCATTCCCCT GGGAATGTTGGCTGGCTCTAG TGAGTAAAAACTCATTAATGGTTGTTTTTGAAGGGAATGTTGGCTGGCTCTAGGCATTCACAAGTGTGGGAGAAGAAATCGATCAGAAGCAGTGGAAGCTTTGGACCAGGCTTCATTCCCCTCAATATAATTACCATCTTCTTCTTCTTTT GATTGTCGCNNAATGAGTAAAAACTCATTAATGGTTGTTTTTGAAGGGAATGTTGGCTGGCTCTAGGCATTCACAAGTGTGGGAGAAGAAATCGATCAGAAGCAGTGGAAGCTTTGGACCAGGCTTCATTCCCCTCAATATAATTACCATCTTCTTCTTCTTTTTCTTGGATCGTAAGTCTGATATTTGATGATGAGAGGCTAAGGTTGATGGGAATGTTGTTTGGCTCGAGGCGATATAGGTTTGATCTTTTATCTTTGACTTCTGGGATTATTTTTTCTCGTGAAAATTCAAAGATATATATATATAGCCCTATGTAATCTCGGACCAGGCTTCATTCCCGTCAACCAACCCTTTCTCTATTTCTGATCTGAAAAATCTCTCCTTCCCCTCCAAGAAAATGATGATATGTTTCATTTTCAGTTGATGGTAAGCTCTCTCTCTCTTAGGGTTTGATTCTGTCTCTTTCTCTCTGGAGAAGTTTCTGCGGATGGACTTTGCATGGAGTCATTTTCTGAACCACTTGTCAAATATGCTAACAAACAAGACCAGAAGTTACCTCTGACAGATTTCTGCTTTACCTTTTTGGGTTGACCATGATTTCTTCATTTCTTTCTTTCACTTTTCACAACATTCACACAAAAAAAAAAAAAAAGACAGAAAATTTGGGAAAAGATAGAGAAGCTGTGATTTGGGATT 4 2 3' arm Manihot_esculenta mirnest mir-166 0.0000001 vvi-MIR166b 0.0000000005 -46.1 (((((....)))))...(((((.(((.((((.(((((((..((((((.((.((((.(((((..((.(((..((.....))..)))...)).))))).)))).)).))).))).))))))).))))...))).))))).............. (-46.1)