mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure AAGCTGTTCGACGAAATGCCT TACTTTGTCGCCCGTCTTAT ATTATCGAGCATCAAAAACACTGCCCAGATATTCGTAATGAGTACTTTGTCGCCCGTCTTATTCTTCTTTATGGCAAAGCCAAAATGTATGATAATGCCCGTAAGCTGTTCGACGAAATGCCTCAATTAAAATGTCCGCGCTTTGTCTTTTCCTTCAATGCC CGCCAACGCTTCCATTATGAAATTACCGTTCGCCAACTTGCCGACGCCAACCATTTCTCCGGCATTGAAGATATTATCGAGCATCAAAAACACTGCCCAGATATTCGTAATGAGTACTTTGTCGCCCGTCTTATTCTTCTTTATGGCAAAGCCAAAATGTATGATAATGCCCGTAAGCTGTTCGACGAAATGCCTCAATTAAAATGTCCGCGCTTTGTCTTTTCCTTCAATGCCCTTTTGGAGGCATGTCTTAAGTCGGAGAGATATGATAAAATTGGCGGGTTATTTCGCGAATTGCCCCAGAAATTGTCAATTGAGCCTGATCTAGTGTCCTATAACACTGCCATTAAGGCATTATGTAAGGCTGGTTCATTGGATTCTGCCGTGTATTTTATGGATGAGATGGAAAAGCATGAGATCAAGCCGAATATTGTGACTTGTAATACGTTATTACACGCATTTCAT 4 1 3' arm Nicotiana_tabacum mirnest 100 100 -24.8 ........((((..((((.((.((...((((((..(((((((((.(((((((..((.(((((((....((.((((...)))).)).....))........))))).))..))))))).)).))).)))).))))))...))...)).))))......)))). (-24.8)