mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GCTCTTCTTCTTCCTCTTCCT GAGTGAGAATGAGAGGAGACC CAAGAAATGTCGCTCTTCTTCTTCCTCTTCCTTAGCTTCCTCGACCTCTTCTAAAAGAGTCTCTAAAGAGGGAAAAATGGTGTCGAGGATAAGGTCCAACGACATCACAAAGAGGTCTACACGGCAACAGAGAGTGGTTAGGAGTGAGAATGAGAGGAGACCAAAGTCGA AGCATAACGAGAAAGGGCAAGAAAGGAACAAAACCCTGAAAATAAACGAAGAAAATGGCAAAGAAAGCTTCAAAATCCAAGAAATGTCGCTCTTCTTCTTCCTCTTCCTTAGCTTCCTCGACCTCTTCTAAAAGAGTCTCTAAAGAGGGAAAAATGGTGTCGAGGATAAGGTCCAACGACATCACAAAGAGGTCTACACGGCAACAGAGAGTGGTTAGGAGTGAGAATGAGAGGAGACCAAAGTCGAAGACCAAGAAAAATAAGAAATTTGATGCTAAAAAGCCCAAGAAACCTCCAACTGCTTTCTTTTACTTCTTAGAGGATTTCCGCAAGGAATTCCAAGAGCAGAATCCAGATGTCAAGTCAATGCGTGAGGTTGGCAAGGCATGTGGAGAAAAGTGGAAAACAATGACCTATGAGGAAAAGGTTAAATATTATGATATAGCCACAGAAAAGAGAGCGGAGTTTGATAGAGCCACGTCAGAATATATCAAGAGAAAGGAAAGCGGTGAAGATGAAGACCCTGAGGATGCCTCTGAGTTTGACGATTAGAGTTCAAGGTTGTGTTTGAAAACCGTTGTTTCACTAGGAGTACATGAGGAAAAAAATGTATATCTACTTT 2 2 5' arm Populus_nigra mirnest 100 100 -43.6 ...((.......((((((((.(((.(((((((.((((..(((((((((((((.....))(((((....)))))....((((((((.((((...))))..))))))))..))))))))..............))).))))))))).))))).)))))))).......)).. (-43.6)