mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GAAAGTGTTTTCCGTTGACCA ATTTTCCGGAAACCACTTTCTG TGAAAGTTTAGAAAGTGATTTTCCGGAAACCACTTTCTGAAAAATAAACACAGCCAAAAGAGAATATTTTCTTGGAAACCAAGTCAAATTTTTCTTTGACTGGAAAGTGTTTTTCGTTAACCAGAAAGTGTTTTCCGTTGACCACCTTTTCTAATGGC ATTGACCAACTTTTCTAATAGCAAATAAACACATGAAAGTTTAGAAAGTGATTTTCCGGAAACCACTTTCTGAAAAATAAACACAGCCAAAAGAGAATATTTTCTTGGAAACCAAGTCAAATTTTTCTTTGACTGGAAAGTGTTTTTCGTTAACCAGAAAGTGTTTTCCGTTGACCACCTTTTCTAATGGCAAACAAACACAGGAAAATTTAAAAAATAATTTTCAAAAACTACTTTTCAAGAAACAAACATAGCCTTAAGTACTAGGATTATTCTGGACCTCTTTGACAGCTAGAGGTTCATACCATTGAGGACCAAATCCAGCAGCTTTCCTTGCTTCCTCGTTAAAGGGAGGCTTCAATGGTCCTCTGAAGTGTGTTCTCACTATAGCATGAAATTTCTGAATCACCTCTTCATTCCCCTCCGTAGAAATTTCAGTTTCCTTGTCTCCATTTTCTTCAGATGACAAGCTGTCTCTACTTAAAGCTGGAGTTTTAGACCTCAAGCATAGATACTTAAACCACTTCACTCCAGCTGCACAATGGGTTATCTCTTCT 4 1 3' arm Populus_nigra mirnest 100 100 -42.7 .......((((((((((.((...((((((.(((((((((.((...((((((..(((..((((((.((((.(((((...)))))..))))..))))))...)))...))))))....))...))))))))).))))))..)).)))..))))))).... (-42.7)