mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGGCAGGGCAATTCTCCCTTG AAGGAGGATAAAGCCCTGTTATT TCAAGTAACTGTGGAATCGAATGGCAGGGCAATTCTCCCTTGGCAAAACAGAAATATGCAGGCATTTATTTCCATGTTCTGTTTGCCAAAGGAGGATAAAGCCCTGTTATTCTTTAGCCATAAATGATA TCCAGTTGATCGGAAAGTGCTAGCCCTCATCGATTCATACTGCAATATTTTTCTGACATTTCTGCTAAAAAGTGAAATAAAAGATTATTTTTATGCAAGGGAGATAAAAAATCTGACCCAAAAGTAATTGCAAGAGATATCATATCATGATGATATTCATGAAGAACAAGAGACGCATTCAAGTAACTGTGGAATCGAATGGCAGGGCAATTCTCCCTTGGCAAAACAGAAATATGCAGGCATTTATTTCCATGTTCTGTTTGCCAAAGGAGGATAAAGCCCTGTTATTCTTTAGCCATAAATGATAGATGCATTGCCTAGCTCCATCATCAGCCAGCGAGCTATAGCTAGCATTGCTCGAGTTCC 2 2 5' arm Populus_tremula mirnest mir-399 2e-49 ptc-MIR399f 4e-50 -46 .........(((((....((((((((((((.(((((((.((((((((.(((((.((((.(((......))).))))))))))))))))).)))))))...)))))))))))).....)))))....... (-46)