mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure AAAATACTCCCTCCGTCCGGA TGGACGGAGGTAGTATATGTT CAGTCGCCAATAAAATACTCCCTCCGTCCGGAAATATTTGTCTAAGAGATAGATGTATCTAGACGTATTTTAGTTCTAGATACATCCTTTTTATCCATTTCGAAGACAAGTATTTCTGGACGGAGGTAGTATATGTTTGAAG ACCGATTTTTCTTTTTTTTTTTTAATAAAGAAAACAACGGAATAAACACTGGATATTTCCCCACAGAAAGAGAAGCACTGAATATTTACATGCCCTAGTCATCTAGTCTGCAGCAGCAGAGACAGGCTCAAACGCTACAAGTTTTGATCTAACAAAGCACACAACGCACGTCATATCCCAGTTTCTTGAGTAGCTCCATCCTTCCTCGCACGCCATTTACCCAAGTCTACGACACATCCAACCACAAACTTCTGCGCAATCTCTACGACGGTGGAGATTGCGCACAAGTTTCCTGGTTACGTGCCGTAGAAATTCCATGAACAACCCTTCACGTCGCGGTGCAATTGCAGCCTCAGAAATCCAGAGGTGGCGTCTCCTGGTACATTGGCAGAATACATGTCAATACTATCGTCAGAGCTGAATTAGTTAGACCTGAACACCACCTAAGAAACAAAATAGACCTGAACAATTACACCAAATATGAGGAGAACCTAATCTAGTTGTATTTCAATCAGTCGCCAATAAAATACTCCCTCCGTCCGGAAATATTTGTCTAAGAGATAGATGTATCTAGACGTATTTTAGTTCTAGATACATCCTTTTTATCCATTTCGAAGACAAGTATTTCTGGACGGAGGTAGTATATGTTTGAAGGCCATAAATAATCATCTGCGAGCAATTATGTCCACAGAAACACCATTTAATAAGCTCATAGTTTGGACATTATCCAGGAGCGACAGTGAAGACCTTACCTTGTGCAACAAGTCGTGGGCAATGAAGTCCCAAG 4 0 5' arm Pseudoroegneria_spicata mirnest MIR1122 2e-28 tae-MIR1135 8e-21 -55.4 .......(((....(((((.(((((((((((((((((((((((..(((((.((((((((((((...((....))))))))))))))..........)))))..))))))))))))))))))))))).)))))....)))... (-55.4)