mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GGTGGTTAAGTTGGTTCATAT ATAGATCAGCTCGACTTTCTA CCAACTATAGATCAGCTCGACTTTCTAATGGTGGTTAAGTTGGTTCATATTAAGTTAAG CCAACTATAGATCAGCTCGACTTTCTAATGGTGGTTAAGTTGGTTCATATTAAGTTAAGCCTCCCAGGACAAACATCCGATAATGACTCAAGCCAACCCAACATTGGAGTCCTTATCAACTGGCCATCAGTAAGAAAGGACTCTTATTTTCCATTCAACCCAAGAAGCTGTTAGTGTCCCAAGTTTTTTTGTCTTGCTGCCATTTAAACCATCCCCAAGGTTTGGGGAAAACTTCTTCATGTGTCCATTCTTCATCTGTGTGATGGCTGAAGGATCCTGAGATCTTGTCCTGTACAGGAACCTTCTGCTTTCAAAGGAGTATCAGTGGTGTGTATTTATGTGGTTAAACAAACAACTTACCTATCACTTCTTGTACTTATCTAGTTTAGGTGGACAGTATATAATTGTCAGGACTGATGGCACATCCAAGCATCACTTTGCTTTCGCCATGTCATCAAACCGAGTAGTAACGAGTATAGGCAATCGGAGGAGGAAAGTAATTCATGTTTACAAACCGCCTGTAACAAGAAAGATTGGACTGTTTCCCAGCACTAGAATAGTCCAAATGCCACTCACACAGCCCTGCAGTAACCCCCGGCTATGGTTTTACGCTGAGTTACCCCAAACACGGGCTGTAAATAAACTGTATGGAAAGATATTCCCTTGCCAAGCTCTTACTCTTACAGAGGTATTTTCCAACTGAAAACTACGTTTTATGCACTGTTTTTTATTTTTTTACTTTCACAGCAGCCTTTCTATCTCCCCTCCATCAAAAGTATAAAACTGCAATGACTATCTATTACTACTAATAGCACTCTCTATTCT 4 0 3' arm Xenopus_laevis mirnest 100 100 -12.7 .....(((.((((((((.((((.((....)).)))).)))))))).))).......... (-12.7)