mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GAAAGTGCTTTTTGTTTTGGG CAAAACTAGGCCCTCTCTT TTTTAGATTACCCAAAACTAGGCCCTCTCTTGTGTTTCAATTATTAAGAAAGTGCTTTTTGTTTTGGGCATTGTATG CGCTCCGCAGAAAGCACTGAAGCTCCTCNAGTGACCTCTGCAAGATGTTTCTTGTACACTCCAATATTACTCCNATGGGTTTGGAGTCAAATCTTCNAAGGCCTTGCAAAAAAAATCCTCTGCAGAGTAAAAGAACCAACAGTGGAAAGGGACTGAAGATTCCTACAGGCTGATTATGCATCACCCAGAACAAGGCTCTCTTCTTTTGACCTTCATGGATGGTGAGAAAGTGCATTCTGTTTTGGGTGATCTATGTATCAGAAATACATCAAAGGTTTTTATAAGGGGAATTGGTTCAGATTAGAAATGTATCACTTGGGTTTATACGGGAAATCAATCCTAGAAAGTTGTGAAAGCCTTCCAATGGATTGATAATGTGTCAGATACAACTGATTTTCAATGCAAAGCCAATTTTAGATTACCCAAAACTAGGCCCTCTCTTGTGTTTCAATTATTAAGAAAGTGCTTTTTGTTTTGGGCATTGTATGTTTTGGGCACTCCATTTAGAAATCCACCCTTTCCAAGAAAAAGCACAATTGTCTGTGGAAGAGCCATTAGCTGACCAATTTGATATCAAAAAAAAAAAAANTANNAAAAAAAATTTTNNAAAANAAA 3 2 3' arm Xenopus_laevis mirnest 100 xtr-mir-427-1 0.0000009 -19.3 ..........((((((((.((((.((.(((((.((....))...))))).)).))))...))))))))......... (-19.3)