mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure AAGTGGAGGTGTGCTCTCTTG GTGGAGCGCAAGTCACAGTC ATCTCCTACCTAAGGACAGCTCTTGGGGTGAAAGTGGAGGTGTGCTCTCTTGCTGGATGTGATTATATTGTCAGCAATCGCTTAGCAGTGGAGCGCAAGTCACAGTCTGGAATTTGCCAACGCTTGCCAAACGGA AACACAAACTACAACNNNNNTGATTNNTCGATTCCCCGGGTGGCATCTAGCGTTCAAGAAGTGGAAATGAACTTTGACCTCCTAAAAGATGACAGCATAATTGGTGGTAAAAAACAGTACTGTACAAGGAGGAGATTGAAGCTCAATGAAGCACAATCAAGACATCGTAGTGATACACAACCTGCCAAAAAGAAAACAAGGCTTATTATTCAGGATGACTCTAGTGAAGAAGAGACAGGAGCAACATCTAAACTGAAGGATACTTCTCATCCTCAAAAGACTTTTCCTTCCTCTAAACCATCTTTGGGTAACACTTCTTCTAGGAACCAAGGTGCTCCCACGCTTATTAGGAATGAATATGATCTACCTCTGCGGGACCGATGCCAAGCAAGGATTAATATCAAGGCCTCGCTTTCTGAGGAACTAGATTTTCAGGCAGCAAGTAAAAGTAGTAGTAGCACTAAATCAAGAACATCAAGCGGGATTGTCCACTCTGAGAGAAAGGGAGAAGTCAGTACTGGAGTGGTTAACTTTGACTTGACCCTGGATAGTTCATCTGTAACAAATCTCTCCTTTCCAAGGAATGATGAAGTATCTGCTCCTACACCAATCAGGACAACTCCATGCATTCTTGCTGACACCCGGGAAATCTCCTCTGGGCCCGAGGTCATCTCCTACCTAAGGACAGCTCTTGGGGTGAAAGTGGAGGTGTGCTCTCTTGCTGGATGTGATTATATTGTCAGCAATCGCTTAGCAGTGGAGCGCAAGTCACAGTCTGGAATTTGCCAACGCTTGCCAAACGGAAACAAACTGGGTGGGATCCGGATCCCAGCACCTTGGCAACCATTTGGTTTCAATAAGGGGTTTGGGGGTTGGTTAATAAGAAGAAAGGGAACCCGCAATTTCAAN 5 1 5' arm Xenopus_laevis mirnest 100 100 -43.9 ......(((((.((((....)))).)))))...((((...((((((((.((((((...((((((...........)))))).)))))).))))))))..)))).((.(((.....((....))...))).))... (-43.9)