mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure CTCGTCATCGTCGTCATCGTC AGGTGGTGATGAAAGACCAAGTG TGCAAAGAAGGTGGTGATGAAAGACCAAGTGGAGGAGAGGAATCCCATGTTCACCCATCCCTCGTCATCGTCGTCATCGTCAACAT AGTCAGTTCACTGCATTGGGAATGGCCCCGGGAATCAGAGATGCAAAGAAGGTGGTGATGAAAGACCAAGTGGAGGAGAGGAATCCCATGTTCACCCATCCCTCGTCATCGTCGTCATCGTCAACATCA 3 2 3' arm Ictalurus_punctatus mirnest 100 100 -19.1 ......((.(((((((((((..(((..((.(((((....((((.....))))..)).))))).))).))))))))))).))..... (-19.1)