mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TCAGTTTTCCCAGGATTTTGC GGAATATTTGGAGGAACTTGCG ATCTCACTTTGTCAGTTTTCCCAGGATTTTGCAATTCAGAGGAATATTTGGAGGAACTTGCGATTTTTAA GGATACCAATACGAATGTTTTTTTATTATGGTGAGGATTTGTAAATCTGGATAGAGCGGGAGTCCAAAGCACAACTCTAAAATATCCAAGAAGTACAATATTAAAAAGCAAAATATTAGTAAGACATTTTGTATTTTAAATCAGGACGTCAACAACAAACCGAACAGGAAAAAACAAACAAACATAAAATCAGATCGAGAATGAAAAATACCTTGCAATGAATTCCGTTACAGATCAGTCGTATGGTGCTGTAAAGCATACTACGTCACTTCACGTCGGATAAAAACGCTGGTTTCATGCAGCAAAGTATGTTAAAACAGAATACCTCACGTGTAGGAACACTTTACACTACCCAGCTGGCCTCGTAACGTTTTCACGCGAGTAATATCTCACTTTGTCAGTTTTCCCAGGATTTTGCAATTCAGAGGAATATTTGGAGGAACTTGCGATTTTTAAAATATTACAGAAGATCTGGGCCAAGATGAGCCACATGACGTCACGACGTGCGTTCGGCTGAAGCCCTCTGATTCACGTTCGTCGAACATGAGTACAGATGAAAAGGTCTCATTTACCAACAAACATTGCTGTGAAATTTTGCAGATTCAAGCAGTTTTCCACAAAAATCTCCGTAAATTACGTCGTAGATTTTGAGAAAACCCGCAGCGAAAGCAAGCGTTTTTGGCCGGAACGAT 5 1 5' arm Ictalurus_punctatus mirnest 100 100 -13.3 ........((((.((((((.((((.(((((..........)))))..)))).)))))).))))....... (-13.3)