mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GAAGGAAAAGGAGCTCGCTGT ACTGAGCGGTTTGTCCTTCAG GATGATCCGGACACTGAGCGGTTTGTCCTTCAGGGCTTCCGGGGCGGCGAAGGACTTTGCAACCAGGCGGCTGCACAGCACGTATGATGTGGCCGTGGTGGGGGGCGGCATCGTCGGCCTGGCGACGGTCAGAGAGCTAATTCTGCGACACCCGTCCCTCAGCTTCGTTCTGCTGGAGAAGGAAAAGGAGCTCGCTGTGCACCAGAG CGAGTCCCGCGGACCTTTTGCGCGATGAAGAGGTGAACGCGAAGGGGCTTTGAAACGCGGCGACGATGATCCGGACACTGAGCGGTTTGTCCTTCAGGGCTTCCGGGGCGGCGAAGGACTTTGCAACCAGGCGGCTGCACAGCACGTATGATGTGGCCGTGGTGGGGGGCGGCATCGTCGGCCTGGCGACGGTCAGAGAGCTAATTCTGCGACACCCGTCCCTCAGCTTCGTTCTGCTGGAGAAGGAAAAGGAGCTCGCTGTGCACCAGAGTGGGCACAACAGTGGAGTCATTCACAGCGGGATCTACTACACGCCGGGGTCACTGAAGGCCCGCCTGTGTGTTCGAGGAGCCACTTTAGCCTACGAGTACTGCGAGAAGAAAGGACTCCCTTACAAGAAATGTGGAAAGCTCATCGTGGCTTTGGAGCATGAGGAGATACCCAGACTGCAAGCTCTGTACGAGCGCGGCATGAAAAACAACGTGCGGGACCTCAGCATAATCGACGCCAAGGGAATCCGAGAACGAGAGCCATTCTGCAGGGGTTTAATGGCCTTAGACTCGCCGTACACCGGCATCGTGGACTGGAGGATGGTGGCTCTCCGGTACGGGCAAGACTTTGAGGAGGCAGGCGGCACGGTGGTTACGGATGCTGAGGTCGACGACATTACCACGGTTACCGAGAGCCCGGCCGGGAGTGCTGACGGAATGAAGTATCCAATTGCAGTCAGAGACAAAAAGGGCAATGAGGTCCGCTGCCGTTACGTCCTGACATGTGGCGGCCTGTACTCCGACCGCCTGTCCCAAATATCGGGATGCAGTCCCGAGCCTCGGATCGTCCCCTTCAGAGGAGATTACTTGGNGNTGGAAGCCAGAGAAACACTATCTGGTCCAGGGGAATATCTATCCTGTCCCCGACCCTCGGTTCCCCTTCCTCGGAGTTCACTTCACCCCCAGAATGGATGGGAATGTTGGGCTTGGCCCGAACGCAGTACTCTTCCTTCAGGAGGAGGGCTACCAAGGGGTACCACTTTCAGGTTCAAACCTTGCAAATGCCCTCTCATTCCGGGGCCCTCCAAAACTGGTAATGGAAAAACCGTACCCTTG 3 2 3' arm Gasterosteus_aculeatus mirnest 100 100 -70.9 .....((.(((((.(((((..(((.((((((..(((...((((((.(((((....))))).(((..(((((((((((.......)).))))))))))))(((((((((..((((.((..((((............))))..)).))))...))))))))).))))))....)))...))))))..))).))))).)))...)).)). (-70.9)