mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure GGAGGAACTCCCGGTGGGATT CCCTCCCGGGGGGATTTCAG AAAGCAGCGAGGAGTCGGACAAGACGTTCCGGCGACTCGGGGGAGGAACTCCCGGTGGGATTTTGAAGGGAAACCCTCCCGGGGGGATTTCAGAAAAATTTCTCCCCCCCCNNGNGGNGGAGAGGNGGGGG CTTGCTGNTCNTCNTTGGGACTTGACAGTTCGGTCCGGATTCCGGGATCGCCCGACCAGTCTGTGAGGAACGTGGAGGTCGTTGAGCCCGTGCAAGAGTGGGCGCCGTCGGTCGGGAAGACCAAGAAACCCAAATCCGATGACAAGTTCTACTCGGACAACGAAGAGGAGAAAGCTGAGGAAGAATCTGAGAGCAGTGAAGGAAGCAGCAGCAGCAGCAGCAGCAGTAGTGGAGAGTCNAGCANCGAGAGCGAGGAAGCAAAGCAGCGAGGAGTCGGACAAGACGTTCCGGCGACTCGGGGGAGGAACTCCCGGTGGGATTTTGAAGGGAAACCCTCCCGGGGGGATTTCAGAAAAATTTCTCCCCCCCCNNGNGGNGGAGAGGNGGGGGGNGGTNGGGGGGGGGGGGGGGGGGGGGAAAAGAGTGGGAAAGAGAGGGGTCCCCACCCCCCCCCCCCCCCNAAAAAAAAAAAAAAAGGGAGGAGGGGGGGGGGGGGGGCCCCCCCCCCCCCCCTCCCCCCTTCNNCCCCCNNANNNNNNNNNNNNNNNNNNNGANAGGAAAAAAAAAAAAAAAANAGGNGTNGNNNNGNGGNGGGGTNTTGTTTGCNTGCGCCCGTGGGGAAGGGA 4 1 5' arm Gasterosteus_aculeatus mirnest 100 100 -45.5 .......((((..(((((((.....).))))))..))))..((((...(((((((.(((.((((.....)))).))).)))))))..)))).(((....))).(((((.((............)).))))) (-45.5)