mature_miRNA miRNA* pre-miRNA EST_contig mismatches bulges arm species source_database RFAM_best_hit RFAM_lowest_evalue miRBase_best_hit miRBase_lowest_evalue folding_energy secondary_structure TGTGTGTGTGTACATGCACAC GTGCATGTATAGATAGATAGA TCATCAAAAGTCGCTTCTACATGTGCATGTATAGATAGATAGATGTACATATGTGTGTGTGTACATGCACACCTTGAAGTACATCTTGAAG GGGGNNNNNCCCCTTTCCTGCTTTTTCGAACCATAAAGAAAAGAGCGTCACAGCCAGGGGGGATTATGGGTAACGTAGTCTAGGAGACCGTGGAGAGAGAAGGGAACGGAATCTGTTTACTGACCACAAGCTTCTGGTGCTGATGGGAGATGTAACCTTTGATGTGACCCATGTAGATGAGGTTAGCCAGGATACACTGAACCTCGTCGAGGTCCACGTCGTCCAACTGCATCATCTTCATGGCCACCACAAAGGCATCCAGAGGTAGCTGGTGAGTCCTCAGCAGAAGATACACTTTCTTGAAGAGGTTCCTGTAGGTGATGATCTTCAGCTTCTCCAGGATGAAAAAGATTCCGCAGCGGATGAAGAAGGTCTCGTGTTTGGCCAAGGCCTCGTTGAGTAGCAGTAGGTTTCCCTCACTCACAGATTTGGTGACATCTGCAAACTGCATGAGGTCGTATTTCCTCAGGAGCTGATGAGTCGGCATGTGACCCAGGAGCATCTTGACAGGCAGCAGGTAGATGAGGATCATCCTCTTGTTCTTCTGACTGGATCGATGACAGTGATGGAAAGCATACGACAGGAACTCCTCCGCTGGCTTGAAGTCGCTGTCGAACATGGCCTTGCGGCCCACGTAGTACTTGTACGTGACCTTCTGGGCCGGGCTGTAGTCGTTTTTCAGGTTGGAGCTATCGATGGCTCTGATCAGAGGCTTACACAGGTGGAGCTTATTAATCTAATGAGGACAGTTTTCATCAAAAGTCGCTTCTACATGTGCATGTATAGATAGATAGATGTACATATGTGTGTGTGTACATGCACACCTTGAAGTACATCTTGAAGAGCTGGTTGCTCAGAAACATCATCCCCCACTTACTAGAGTCCTCGATGCCGGCTCGACTGAGGGCCAGAGCAGCACATGGTT 3 0 3' arm Gasterosteus_aculeatus mirnest 100 100 -23.3 ...((((..((.(((((....((((((((((((.(((.(((..........))).))).))))))))))))....)))))))...)))).. (-23.3)